1. Development of a selective, sensitive and robust oxyntomodulin dual monoclonal antibody immunoassay.
- Author
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Umberger TS, Ming W, Cox JM, Konrad RJ, and Siegel RW
- Subjects
- Humans, Proglucagon, Glucagon, Protein Precursors analysis, Glucagon-Like Peptide 1, Immunoassay, Oxyntomodulin, Antibodies, Monoclonal
- Abstract
Background & Aim: Oxyntomodulin (Oxm) is a proglucagon-derived peptide agonist of both the GLP-1 and glucagon receptors and is a key regulator of gastric acid secretion and energy expenditure. Differential processing from proglucagon hinders assay immunoassay selectivity. Method & results: Antibody engineering was used to develop a sandwich immunoassay that selectively measures endogenous Oxm. The pre- and postprandial levels of Oxm from 19 healthy individuals over the course of 2 h were measured. Postprandial increases in Oxm occurred within minutes and levels significantly correlated with those obtained using previously published mass spectrometry assays. Conclusion: This sandwich immunoassay is appropriately sensitive and selective and is also amenable to high-throughput application for the reliable determination of endogenous levels of intact Oxm from human samples.
- Published
- 2022
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