1. Hapten synthesis, monoclonal antibody production and immunoassay development for analyzing spiropidion residues.
- Author
-
Jin Y, Jin Z, Sun D, Liu Y, Xu B, Zhao Q, He Y, Gong L, Li J, Zhang Y, and Cui Y
- Subjects
- Animals, Tandem Mass Spectrometry, Food Contamination analysis, Mice, Inbred BALB C, Mice, Citrus chemistry, Insecticides chemistry, Insecticides analysis, Antibodies, Monoclonal chemistry, Antibodies, Monoclonal immunology, Haptens chemistry, Haptens immunology, Enzyme-Linked Immunosorbent Assay, Pesticide Residues analysis, Pesticide Residues chemistry
- Abstract
Spiropidion developed by Syngenta shows high insecticidal and acaricidal activity against a wide range of sucking pests. In this study, according to the structure of spiropidion, two haptens were synthesized by introducing carboxyl groups from the ester group. After cell fusion, a monoclonal antibody (mAb 8B5) of spiropidion was obtained. The IC
50 of the established heterologous indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was 7.36 ng/mL, and its working range was 1.75-34.92 ng/mL. The average recoveries were 76.05-124.78% in the Yangtze River and citrus samples. Moreover, the ic-ELISA results of 15 citrus samples agreed well with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Overall, the established ic-ELISA could be applied for the spiropidion residue monitor in food and agricultural samples., Competing Interests: Declaration of competing interest The authors certify that none of their known financial conflicts of interest or close personalties could have appeared to have influenced their search presented in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF