1. A monoclonal blocking-ELISA for detection of orthopoxvirus antibodies in feline sera.
- Author
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Czerny CP, Wagner K, Gessler K, Mayr A, and Kaaden OR
- Subjects
- Animals, Antibodies, Monoclonal immunology, Antibodies, Viral immunology, Antibody Specificity, Binding, Competitive, Blotting, Western, Cats, Germany epidemiology, Hybridomas, Mice, Neutralization Tests veterinary, Poxviridae Infections epidemiology, Reproducibility of Results, Sensitivity and Specificity, Seroepidemiologic Studies, Antibodies, Viral blood, Cat Diseases epidemiology, Enzyme-Linked Immunosorbent Assay veterinary, Orthopoxvirus immunology, Poxviridae Infections veterinary
- Abstract
A double sandwich blocking-ELISA using a genus-specific neutralizing monoclonal antibody (MAb) against the vaccinia virus 32 kD adsorption protein (D8L open reading frame: ORF) was developed to detect orthopoxvirus (OPV) antibodies in sera. A collection of 2173 feline serum samples was examined in an epidemiological study. The blocking-ELISA revealed 44 (2%) sera with positive titres of 1:2-1:256. ELISA results were confirmed by the plaque-reduction test. A close correlation between titres of both assays could be observed (r = 0.986). In general, the sensitivity of the blocking ELISA was two to four times higher. Neutralizing OPV-antibodies were found in nine sera with ELISA-titres > 1:4. Antibody specificity to OPV was also demonstrated by Western blotting analysis with selected feline sera. The epidemiographical distribution of the ELISA-positive sera and case histories of 37 seropositive cats available from the referring veterinarians are demonstrated. The blocking-ELISA enables a rapid serological diagnosis and can be used in veterinary and human medicine. It allows OPV-antibody screening in human and other animal species.
- Published
- 1996
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