1. Novel Approach of Vaccination against Brucella abortus 544 based on a Combination of Fusion Proteins, Human Serum Albumin and Brucella abortus Lipopolysaccharides
- Author
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Mohammad Raftari, A. Rezaee, Sobhan Ghafurian, Mohammad Javad Rasaee, I. Pakzad, A. Hosseini, Ahmed Sahib Abdulamir, B. Tabbaraee, and F. Abu Bakar
- Subjects
Lipopolysaccharide ,Antibody titer ,Virulence ,Spleen ,Brucellosis ,Cell Biology ,Biology ,medicine.disease ,Virology ,Immunoglobulin G ,Microbiology ,Vaccination ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Toxicity ,medicine ,biology.protein ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) - Abstract
Lipopolysaccharide (LPS) of Brucella abortus is an essential component for developing the subunit vaccine against brucellosis. B. abortus LPS was extracted by n-butanol, purified by ultracentrifugation and detoxified by alkaline treatment. Pyrogenicity and toxicity of B. abortus LPS and detoxified-LPS (D-LPS) were analyzed and compared with LPS of E. coli. Different groups of mice were immunized intraperitoneally with purified B. abortus LPS, D-LPS, a combination of LPS with human serum albumin (LPS-HSA) and B. abortus S19 bacteria; besides, control mice were inoculated with sterile saline. Two doses of vaccine were given 4 weeks apart. Mice were challenged intraperitoneally with virulent B. abortus 544 strain 4 weeks after the second dose of vaccine. Sera and spleens of mice were harvested 4 weeks after challenge. LPS-B. abortus was 10,000-fold less potent in LAL test and 100-fold less potent in eliciting fever in rabbits than in E. coli LPS. And D-LPS was very less potent in LAL test and eliciting fever in rabbits ordinary LPS. The antibody titer of anti-LPS immunoglobulin G (IgG) was higher than D-LPS. However, mice immunized with either LPS, D-LPS or LPS-HSA vaccines showed a significant protection against infection of the spleen (p
- Published
- 2010