5 results on '"Guirado, Ramon"'
Search Results
2. Effects of PSA Removal from NCAM on the Critical Period Plasticity Triggered by the Antidepressant Fluoxetine in the Visual Cortex.
- Author
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Guirado, Ramon, La Terra, Danilo, Bourguignon, Mathieu, Carceller, Hector, Umemori, Juzoh, Sipilä, Pia, Nacher, Juan, Castrén, Eero, DiCristo, Graziella, and Hildebrandt, Herbert
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NEURAL cell adhesion molecule ,NEUROPLASTICITY ,ANTIDEPRESSANTS ,VISUAL cortex ,INTERNEURONS ,PHYSIOLOGY - Abstract
Neuronal plasticity peaks during critical periods of postnatal development and is reduced towards adulthood. Recent data suggests that windows of juvenile-like plasticity can be triggered in the adult brain by antidepressant drugs such as Fluoxetine. Although the exact mechanisms of how Fluoxetine promotes such plasticity remains unknown, several studies indicate that inhibitory circuits play an important role. The polysialylated form of the neural cell adhesion molecules (PSA-NCAM) has been suggested to mediate the effects of Fluoxetine and it is expressed in the adult brain by mature interneurons. Moreover, the enzymatic removal of PSA by neuroaminidase-N not only affects the structure of interneurons but also has been shown to play a role in the onset of critical periods during development. We have here used ocular dominance plasticity in the mouse visual cortex as a model to investigate whether removal of PSA might influence the Fluoxetine-induced plasticity. We demonstrate that PSA removal in the adult visual cortex alters neither the baseline ocular dominance, nor the fluoxetine-induced shift in the ocular dominance. We also show that both chronic Fluoxetine treatment and PSA removal independently increase the basal FosB expression in parvalbumin (PV) interneurons in the primary visual cortex. Therefore, our data suggest that although PSA-NCAM regulates inhibitory circuitry, it is not required for the reactivation of juvenilelike plasticity triggered by Fluoxetine. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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3. Chronic fluoxetine treatment alters the structure, connectivity and plasticity of cortical interneurons.
- Author
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Guirado, Ramon, Perez-Rando, Marta, Sanchez-Matarredona, David, Castrén, Eero, and Nacher, Juan
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FLUOXETINE ,NEUROPLASTICITY ,ANTIDEPRESSANTS ,SEROTONIN uptake inhibitors ,PREFRONTAL cortex ,NEURAL cell adhesion molecule ,THERAPEUTICS - Abstract
Novel hypotheses suggest that antidepressants, such as the selective serotonin reuptake inhibitor fluoxetine, induce neuronal structural plasticity, resembling that of the juvenile brain, although the underlying mechanisms of this reopening of the critical periods still remain unclear. However, recent studies suggest that inhibitory networks play an important role in this structural plast2city induced by fluoxetine. For this reason we have analysed the effects of a chronic fluoxetine treatment in the hippocampus and medial prefrontal cortex (mPFC) of transgenic mice displaying eGFP labelled interneurons. We have found an increase in the expression of molecules related to critical period plasticity, such as the polysialylated form of the neural cell adhesion molecule (PSA-NCAM), GAD67/65 and synaptophysin, as well as a reduction in the number of parvalbumin expressing interneurons surrounded by perineuronal nets. We have also described a trend towards decrease in the perisomatic inhibitory puncta on pyramidal neurons in the mPFC and an increase in the density of inhibitory puncta on eGFP interneurons. Finally, we have found that chronic fluoxetine treatment affects the structure of interneurons in the mPFC, increasing their dendritic spine density. The present study provides evidence indicating that fluoxetine promotes structural changes in the inhibitory neurons of the adult cerebral cortex, probably through alterations in plasticity-related molecules of neurons or the extracellular matrix surrounding them, which are present in interneurons and are known to be crucial for the development of the critical periods of plasticity in the juvenile brain. [ABSTRACT FROM AUTHOR]
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- 2014
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4. Chronic fluoxetine treatment in middle-aged rats induces changes in the expression of plasticityrelated molecules and in neurogenesis.
- Author
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Guirado, Ramon, Sanchez-Matarredona, David, Varea, Emilo, Crespo, Carlos, Blasco-Ibáñez, José Miguel, and Nacher, Juan
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FLUOXETINE , *LABORATORY rats , *ANTIDEPRESSANTS , *SEROTONIN uptake inhibitors , *DEVELOPMENTAL neurobiology , *GLUTAMIC acid , *CELL adhesion - Abstract
Background: Antidepressants promote neuronal structural plasticity in young-adult rodents, but little is known of their effects on older animals. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) may mediate these structural changes through its anti-adhesive properties. PSA-NCAM is expressed in immature neurons and in a subpopulation of mature interneurons and its expression is modulated by antidepressants in the telencephalon of young-adult rodents. Results: We have analyzed the effects of 14 days of fluoxetine treatment on the density of puncta expressing PSANCAM and different presynaptic markers in the medial prefrontal cortex, hippocampus and amygdala of middleaged (8 months old) rats. The density of puncta expressing PSA-NCAM increased in the dorsal cingulate cortex, as well as in different hippocampal and amygdaloid regions. In these later regions there were also increases in the density of puncta expressing glutamic acid decarboxylase 65/67 (GAD6), synaptophysin (SYN), PSA-NCAM/SYN and PSA-NCAM/GAD6, but a decrease of those expressing vesicular glutamate transporter 1 (VGluT1). Since there is controversy on the effects of antidepressants on neurogenesis during aging, we analyzed the number of proliferating cells expressing Ki67 and that of immature neurons expressing doublecortin or PSA-NCAM. No significant changes were found in the subgranular zone, but the number of proliferating cells decreased in the subventricular zone. Conclusions: These results indicate that the effects of fluoxetine in middle-aged rats are different to those previously described in young-adult animals, being more restricted in the mPFC and even following an opposite direction in the amygdala or the subventricular zone. [ABSTRACT FROM AUTHOR]
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- 2012
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5. Effects of the Antidepressant Fluoxetine on the Somatostatin Interneurons in the Basolateral Amygdala.
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Carceller, Hector, Perez-Rando, Marta, Castren, Eero, Nacher, Juan, and Guirado, Ramon
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ANTIDEPRESSANTS , *FLUOXETINE , *INTERNEURONS , *AMYGDALOID body , *PHARMACODYNAMICS - Abstract
Although the precise mechanism of action of antidepressant drugs remains elusive, the neuroplastic hypothesis has gained acceptance during the last two decades. Several studies have shown that treatment with antidepressants such as Fluoxetine is associated with enhanced plasticity in control animals, especially in regions such as the visual cortex, the hippocampus and the medial prefrontal cortex. More recently, the basolateral amygdala has been shown to be affected by Fluoxetine leading to a reopening of critical period-like plasticity in the fear and aggression circuits. One of the key elements triggering this type of brain plasticity are inhibitory networks, especially parvalbumin interneurons. However, recent work on fast-acting antidepressants has shown also an important role for somatostatin interneurons. Here we show that Fluoxetine reorganizes inhibitory circuits through increased expression of the plasticity-related molecule PSA-NCAM which regulates interneuronal structure and connectivity. In addition, we demonstrate that treatment with this antidepressant alters the structure of somatostatin interneurons both at the level of dendritic spines and of axonal en passant boutons . Our findings suggest that new strategies targeting somatostatin interneuron activity might help us to better understand depression and the action of antidepressants. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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