1. Migratory dendritic cells transfer antigen to a lymph node-resident dendritic cell population for efficient CTL priming.
- Author
-
Allan RS, Waithman J, Bedoui S, Jones CM, Villadangos JA, Zhan Y, Lew AM, Shortman K, Heath WR, and Carbone FR
- Subjects
- Animals, Bone Marrow immunology, Cells, Cultured, Herpes Simplex immunology, Herpes Simplex virology, Lymph Nodes immunology, Mice, Mice, Transgenic, Simplexvirus immunology, Antigens immunology, Cell Movement, Cross-Priming, Dendritic Cells cytology, Dendritic Cells immunology, Lymph Nodes cytology, T-Lymphocytes, Cytotoxic immunology
- Abstract
Skin dendritic cells (DCs) are thought to act as key initiators of local T cell immunity. Here we show that after skin infection with herpes simplex virus (HSV), cytotoxic T lymphocyte (CTL) activation required MHC class I-restricted presentation by nonmigratory CD8(+) DCs rather than skin-derived DCs. Despite a lack of direct presentation by migratory DCs, blocking their egress from infected skin substantially inhibited class I-restricted presentation and HSV-specific CTL responses. These results support the argument for initial transport of antigen by migrating DCs, followed by its transfer to the lymphoid-resident DCs for presentation and CTL priming. Given that relatively robust CTL responses were seen with small numbers of skin-emigrant DCs, we propose that this inter-DC antigen transfer functions to amplify presentation across a larger network of lymphoid-resident DCs for efficient T cell activation.
- Published
- 2006
- Full Text
- View/download PDF