1. Isolation, characterisation and complement fixation activity of acidic polysaccharides from
- Author
-
Adama, Dénou, Adiaratou, Togola, Kari Tvete, Inngjerdingen, Nastaran, Moussavi, Frode, Rise, Yuan Feng, Zou, Dalen G, Dafam, Elijah I, Nep, Abubakar, Ahmed, Taiwo E, Alemika, Drissa, Diallo, Rokia, Sanogo, and Berit Smestad, Paulsen
- Subjects
Antimalarials ,Polymers ,Polysaccharides ,Monosaccharides ,Argemone ,Galactose ,Humans ,Complement System Proteins ,Mali ,Arabinose ,Rhamnose - Abstract
Global studies onThis study determines the structure and the immunomodulatory activity of polysaccharides from aerial parts ofAcidic polysaccharides from this plant material named HMAmA1 and HMAmA2 were isolated from water extracts. Their monosaccharide composition was determined by gas chromatography. Glycosidic linkages were determined using GC-MS. NMR was also applied. The polymers were tested for effects on the human complement systemThe monosaccharide composition showed that the two polysaccharides contained in different amounts the following monomers: arabinose, rhamnose, galactose, and galacturonic acid. Overall structural analysis showed the presence of a low ratio of 1,2-linked rhamnose compared to 1,4-linked galacturonic acid with arabinogalactans substituted on position 4 of rhamnose. NMR data showed the presence of galacturonans alternated by rhamnogalacturonans bearing arabinose and galactose units. α-Linkages were found for l-arabinose, l-rhamnose and d-galacturonic acid, while β-linkages were found for d-galactose. The two polysaccharides exhibited strong complement fixation activities, with HMAmA1 being the highest potent fraction. ICHPolysaccharides form
- Published
- 2022