1. Bullatacin, a potent antitumor annonaceous acetogenin, inhibits proliferation of human hepatocarcinoma cell line 2.2.15 by apoptosis induction.
- Author
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Chih HW, Chiu HF, Tang KS, Chang FR, and Wu YC
- Subjects
- Annexin A5 metabolism, Antineoplastic Agents, Phytogenic therapeutic use, Carcinoma, Hepatocellular drug therapy, Cell Death drug effects, Cell Division drug effects, Culture Media, Conditioned metabolism, DNA biosynthesis, DNA drug effects, Dose-Response Relationship, Drug, Ficoll analogs & derivatives, Ficoll metabolism, Flow Cytometry, Fluorescein-5-isothiocyanate analogs & derivatives, Fluorescein-5-isothiocyanate metabolism, Furans therapeutic use, Hepatitis B Surface Antigens metabolism, Humans, Liver Neoplasms drug therapy, Propidium metabolism, Tumor Cells, Cultured cytology, Tumor Cells, Cultured drug effects, Tumor Cells, Cultured pathology, Antineoplastic Agents, Phytogenic pharmacology, Apoptosis drug effects, Carcinoma, Hepatocellular pathology, Furans pharmacology, Liver Neoplasms pathology
- Abstract
Bullatacin, isolated from the fruit of Annona atemoya, is one of the most potentially effective antitumor annonaceous acetogenins. Bullatacin was studied here for its ability to inhibit the proliferation of 2.2.15 cells, hepatitis B virus (HBV) DNA transfected human hepatocarcinoma cell line. It was found that bullatacin induced cytotoxicity of 2.2.15 cells in a time- and dose-dependent manner. Fifty percent effective dose (ED50) on day 1 of exposure to bullatacin were 7.8 +/- 2.5 nM for 2.2.15 cells. [3H]-Thymidine incorporation assays showed almost the same results. Bullatacin-treatment also reduced concentrations of hepatitis B surface antigen (HBsAg) in the cultured medium released from 2.2.15 cells, coincident with the decrease in the cell proliferation. Analysis of mophological changes of bullatacin-treated 2.2.15 by inverted phase-contrast microscope and eletron microscopy revealed a possible model of action for bullatacin to inhibit proliferation of 2.2.15 cells by inducing apoptosis. Most of the bullatacin-induced cell death was found to be due to apoptosis, as determined by double staining with fluorescein-isothiocyanate (FITC)-labeled annexin V and propidium iodide (PI).
- Published
- 2001
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