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Your search keyword '"Madsen JW"' showing total 2 results
Start Over Author "Madsen JW" Topic antineoplastic agents
2 results on '"Madsen JW"'

1. Synthesis and enantiomeric separation of 2-phthalimidino-glutaric acid analogues: potent inhibitors of tumor metastasis.

Author

Shah JH, Swartz GM, Papathanassiu AE, Treston AM, Fogler WE, Madsen JW, and Green SJ

Subjects
Animals, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Drug Screening Assays, Antitumor, Glutarates chemistry, Glutarates pharmacology, Indoles chemistry, Indoles pharmacology, Lung Neoplasms drug therapy, Lung Neoplasms secondary, Melanoma, Experimental drug therapy, Melanoma, Experimental secondary, Mice, Mice, Inbred C57BL, Neoplasm Transplantation, Stereoisomerism, Structure-Activity Relationship, Thalidomide chemistry, Thalidomide pharmacology, Antineoplastic Agents chemical synthesis, Glutarates chemical synthesis, Indoles chemical synthesis, Thalidomide analogs & derivatives, Thalidomide chemical synthesis
Published
1999
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2. A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer.

Author

Sim BK, O'Reilly MS, Liang H, Fortier AH, He W, Madsen JW, Lapcevich R, and Nacy CA

Subjects
Angiostatins, Animals, Antineoplastic Agents chemistry, Antineoplastic Agents metabolism, Cattle, Cell Division drug effects, Dose-Response Relationship, Drug, Endothelium, Vascular drug effects, Immunohistochemistry, Kringles genetics, Kringles physiology, Lung Neoplasms pathology, Lung Neoplasms prevention & control, Lung Neoplasms secondary, Male, Mice, Mice, Inbred C57BL, Neoplasm Transplantation, Neovascularization, Pathologic prevention & control, Peptide Fragments genetics, Peptide Fragments metabolism, Plasminogen genetics, Plasminogen metabolism, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Recombinant Proteins pharmacology, Tumor Cells, Cultured, von Willebrand Factor analysis, Antineoplastic Agents pharmacology, Peptide Fragments pharmacology, Plasminogen pharmacology
Abstract

Endogenous murine angiostatin, identified as an internal fragment of plasminogen, blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. A recombinant protein comprising kringles 1-4 of human plasminogen (amino acids 93-470) expressed in Pichia pastoris had physical properties (molecular size, binding to lysine, reactivity with antibody to kringles 1-3) that mimicked native angiostatin. This recombinant Angiostatin protein inhibited the proliferation of bovine capillary endothelial cells in vitro. Systemic administration of recombinant Angiostatin protein at doses of 1.5 mg/kg suppressed the growth of Lewis lung carcinoma-low metastatic phenotype metastases in C57BL/6 mice by greater than 90%; administration of the recombinant protein at doses of 100 mg/kg also suppressed the growth of primary Lewis lung carcinoma-low metastatic phenotype tumors. These findings demonstrate unambiguously that the antiangiogenic and antitumor activity of endogenous angiostatin resides within kringles 1-4 of plasminogen.

Published
1997

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