1. DNA-adduct levels as a predictor of outcome for NSCLC patients receiving daily cisplatin and radiotherapy.
- Author
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van de Vaart PJ, Belderbos J, de Jong D, Sneeuw KC, Majoor D, Bartelink H, and Begg AC
- Subjects
- Adult, Aged, Analysis of Variance, Apoptosis, Carcinoma, Non-Small-Cell Lung chemistry, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung radiotherapy, Combined Modality Therapy, Female, Humans, Immunohistochemistry, Ki-67 Antigen analysis, Lung Neoplasms chemistry, Lung Neoplasms drug therapy, Lung Neoplasms radiotherapy, Male, Middle Aged, Predictive Value of Tests, Prognosis, Proportional Hazards Models, Proto-Oncogene Proteins c-bcl-2 analysis, Radiation-Sensitizing Agents therapeutic use, Radiotherapy Dosage, Tumor Suppressor Protein p53 analysis, Antineoplastic Agents therapeutic use, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung therapy, Cisplatin therapeutic use, DNA Adducts analysis, DNA, Neoplasm analysis, Lung Neoplasms genetics, Lung Neoplasms therapy
- Abstract
We aimed to investigate whether biological factors related to radiosensitivity and chemosensitivity have prognostic significance in non-small-cell-lung-cancer (NSCLC) patients treated with daily low doses of cisplatin and radiotherapy. We treated 27 NSCLC patients with concomitant daily low-dose cisplatin and radiotherapy between 1993 and 1995. Tumour specimens were analyzed for p53 and bcl-2 expression, and for cell proliferation using antibodies against ki-67. In addition, apoptosis was measured by an end-labeling technique (TUNEL). Finally, cisplatin-induced DNA modification in buccal cells was assessed immunocytochemically using a specific anti-serum. Univariate and multivariate analyses were performed to assess the association between the different variables and survival. The median follow-up was 41 months, and 21 patients (78%) have died. In a univariate analysis, age, tumour stage and cisplatin-DNA-adduct staining were the only factors significantly associated with survival (p < 0.05, log-rank test). p53, bcl-2, Ki-67 and apoptosis showed no relationship with outcome. Multivariate analysis revealed that cisplatin-DNA-adduct staining remained an independent prognostic factor (hazard ratio, 0.10, 95% CI, 0.02-0.49), with shorter survival times for patients with low adduct staining., (Copyright 2000 Wiley-Liss, Inc.)
- Published
- 2000