1. Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry.
- Author
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Zheng LN, Wang M, Zhao LC, Sun BY, Wang B, Chen HQ, Zhao YL, Chai ZF, and Feng WY
- Subjects
- Antineoplastic Agents analysis, Cell Line, Tumor, Cisplatin analysis, Gadolinium analysis, Humans, Neoplasms chemistry, Antineoplastic Agents metabolism, Cisplatin metabolism, Gadolinium metabolism, Mass Spectrometry methods, Nanostructures chemistry, Neoplasms metabolism, Single-Cell Analysis methods
- Abstract
Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.
- Published
- 2015
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