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Interest in proso millet as a functional food is growing. However, studies on the chemical composition, antioxidant, and antiproliferative activities’ differences of proso millet cultivars in China are limited. Nine Chinese proso millet varieties (five waxy varieties - S, D, N2, N4, N8 and four non-waxy varieties - J1, J3, J7, J9) were investigated. Results showed that the non-waxy varieties were significantly higher than waxy varieties in the content of amylase and resistant starch (22.95%, 0.23%, 2.02%, and 0.78%, respectively; P < .05). Lys was the first limit amino acid (AAS: 16.08%). Linolenic acid (61.74%) and oleic acid (22.16%) were the dominant fatty acids. Overall, the antioxidant content and antioxidant activities of J1 and J3 were higher than other cultivars. And, J7, J3, and J1 possessed better antiproliferative effects than other proso millet varieties. These results are anticipated to provide useful information on the development of proso millet-based functional food. [ABSTRACT FROM AUTHOR]

A sensitive electrochemical sensor based on bovine serum albumin (BSA)/poly (diallyldimethylammonium chloride) (PDDA) functionalized graphene nanosheets (PDDA-G) composite film modified glassy carbon electrode (BSA/PDDA-G/GCE) had been developed to investigate the oxidative protein damage and protections of protein from damage by flavonoids. The performance of this sensor was remarkably improved due to excellent electrical conductivity, strong adsorptive ability, and large effective surface area of PDDA-G. The BSA/PDDA-G/GCE displayed the greatest degree of BSA oxidation damage at 40 min incubation time and in the pH 5.0 Fenton reagent system (12.5 mM FeSO 4 , 50 mM H 2 O 2 ). The antioxidant activities of four flavonoids had been compared by fabricated sensor based on the relative peak current ratio of SWV, because flavonoids prevented BSA damage caused by Fenton reagent and affected the BSA signal in a solution containing Co(bpy) 3 3 + . The sensor was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). UV–vis spectrophotometry and FTIR were also used to investigate the generation of hydroxyl radical and BSA damage, respectively. On the basis of results from electrochemical methods, the order of the antioxidant activities of flavonoids is as follows: (+)-catechin > kaempferol > apigenin > naringenin. A novel, direct SWV analytical method for detection of BSA damage and assessment of the antioxidant activities of four flavonoids was developed and this electrochemical method provided a simple, inexpensive and rapid detection of BSA damage and evaluation of the antioxidant activities of samples. [ABSTRACT FROM AUTHOR]