1. Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K + /Na + and ROS Homeostasis in Arabidopsis.
- Author
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Zhang, Ying, Yao, Jun, Yin, Kexin, Liu, Zhe, Zhang, Yanli, Deng, Chen, Liu, Jian, Zhang, Yinan, Hou, Siyuan, Zhang, Huilong, Yu, Dade, Zhao, Nan, Zhao, Rui, and Chen, Shaoliang
- Subjects
SALT tolerance in plants ,EFFECT of salt on plants ,POPLARS ,ROOT growth ,HOMEOSTASIS ,ARABIDOPSIS ,SUPEROXIDE dismutase - Abstract
Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-resistant Populus euphratica and transferred to Arabidopsis thaliana to testify the salt tolerance of transgenic plants. The NaCl treatment (130 mM) reduced the root growth and whole-plant fresh weight of wild-type (WT) A. thaliana, vector controls (VC) and PePLDδ-overexpressed lines, although a less pronounced effect was observed in transgenic plants. Under salt treatment, PePLDδ-transgenic Arabidopsis exhibited lower electrolyte leakage, malondialdehyde content and H
2 O2 levels than WT and VC, resulting from the activated antioxidant enzymes and upregulated transcripts of genes encoding superoxide dismutase, ascorbic acid peroxidase and peroxidase. In addition, PePLDδ-overexpressed plants increased the transcription of genes encoding the plasma membrane Na+ /H+ antiporter (AtSOS1) and H+ -ATPase (AtAHA2), which enabled transgenic plants to proceed with Na+ extrusion and reduce K+ loss under salinity. The capacity to regulate reactive oxygen species (ROS) and K+ /Na+ homeostasis was associated with the abundance of specific PA species in plants overexpressing PePLDδ. PePLDδ-transgenic plants retained a typically higher abundance of PA species, 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2), 36:5 (18:2–18:3) and 36:6 (18:3–18:3), under control and saline conditions. It is noteworthy that PA species 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2) and 36:5 (18:2–18:3) markedly increased in response to NaCl in transgenic plants. In conclusion, we suppose that PePLDδ-derived PA enhanced the salinity tolerance by regulating ROS and K+ /Na+ homeostasis in Arabidopsis. [ABSTRACT FROM AUTHOR]- Published
- 2022
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