1. Structure, physical property and antioxidant activity of catechin grafted Tremella fuciformis polysaccharide.
- Author
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Liu J, Meng CG, Yan YH, Shan YN, Kan J, and Jin CH
- Subjects
- Antioxidants isolation & purification, Free Radical Scavengers chemistry, Free Radical Scavengers pharmacology, Molecular Weight, Oxidation-Reduction drug effects, Proton Magnetic Resonance Spectroscopy, Spectroscopy, Fourier Transform Infrared, Structure-Activity Relationship, Thermogravimetry, X-Ray Diffraction, Antioxidants chemistry, Antioxidants pharmacology, Basidiomycota chemistry, Catechin chemistry, Fungal Polysaccharides chemistry
- Abstract
In this study, structural characterization, physical property and antioxidant activity of catechin grafted Tremella fuciformis polysaccharide (catechin-g-TPS) were investigated. Crude polysaccharides were isolated from the fruit bodies of T. fuciformis and further purified on DEAE-52 and Sepharose CL-4B chromatography to afford a main purified fraction (named TPS). The molecular weight of TPS was determined as 5.82 × 10(5)Da by HPLC. Then, the free radical mediated grafting of catechin onto TPS was achieved by using a redox system. As compared with the unmodified TPS, catechin-g-TPS showed new bands within the range of 1300-1600 cm(-1) in FT-IR spectrum, and exhibited new signals at around δ 6.00 and 6.80 ppm in (1)H NMR spectrum. Thermogravimetric analysis indicated the thermal stability of catechin-g-TPS was higher than TPS. X-ray diffraction spectrum of catechin-g-TPS exhibited two sharp narrow diffraction peaks at 14.2 and 32.1°, corresponding to the crystalline peaks of catechin. Scanning electron microscopy observation revealed the surface of TPS was smooth, whereas the surface of catechin-g-TPS was much rough. These results all confirmed the successful grafting of catechin onto TPS. Moreover, catechin-g-TPS had higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and reducing power as compared to TPS., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
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