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Background/Aim. Rumex crispus (curly dock) is a wild perennial herbaceous plant, which products are considered as a rich source of biologically active molecules with antioxidant and cytotoxic activities. The aim of this study was to estimate of antioxidant and cytotoxic activities of aqueous extract of curly dock fruits. Methods. The aqueous extract of curly dock fruits was evaluated for its antioxidant activity by in vitro assays for ferric-reducing antioxidant power (FRAP), NO⋅, OH⋅ and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging activities and the influence on lipid peroxidation in liposomes. The cytotoxicity of tested extract was examined in vitro in human cervix carcinoma (HeLa), colon adenocarcinoma (HT-29) and breast adenocarcinoma (MCF7) cells. Results. The tested extract showed a potential antioxidant activity manifested in scavenging of free radicals as well as an ability to decrease lipid peroxidation in liposomes. The results indicated tissue-selective cytotoxicity of R. crispus fruit extract in vitro. The most prominent antitumor activity was observed towards HeLa and MCF7 cell lines. Conclusion. The investigated aqueous fruit extract of R. crispus had potential antioxidant and cytotoxic activities, with necrosis as a main mechanism of induced cell-death. Different methods of extraction of R. crispus fruits, apart from aqueous, are recommended for further investigations. [ABSTRACT FROM AUTHOR]

Skin aging is one of the major problems of the modern age. Matrix metalloproteinases (MMPs) are responsible for breaking down extracellular matrix and cause of cell-aging. Ultraviolet (UV) rays and oxidants are also damage skin. Therefore, inhibition of MMP enzymes and higher sunscreen and antioxidant capacity should be a part of treatment strategy in preventing skin aging. The purpose of this study is to investigate the antiaging potential of Rumex crispus L. (Polygonaceae) extracts by determining MMP-1, MMP-8 and MMP-13 inhibitory effects, UV absorption and antioxidant capacities. In this study inhibitory effects of 15 extracts – with different polarities prepared from R. crispus – on MMP-1, MMP-8 and MMP-13 enzymes were determined. Antioxidant capacities were investigated by DPPH, ABTS, NO and phosphomolybdate methods and SPF values were calculated. Also, chemical profiles of extracts were evaluated by HPLC with the compounds we isolated. According to results, aqueous alcoholic extracts showed the highest inhibitory effect on all of MMP enzymes. Ethanol:water (70:30) extracts of roots, leaves and fruits showed high UV protection with SPF 13.56, 15.01 and 15.36 respectively. Total antioxidant capacities were in a range of 49,4%-86,4%. This study shows that Rumex crispus L. provides a valuable and powerful resource for anti-wrinkle, antioxidant and sunscreen cosmetic product. • Rumex crispus L. extracts may be a novel matrix metalloproteinases inhibitor. • Rumex crispus L. root, leaf and fruit extracts have high sun protection factor. • Rumex crispus L. extracts can fight against the reactive oxygen species. • Chemical profile of Rumex crispus L. extracts identified by HPLC. • Rumex crispus L. extracts may be used as a sunscreen and anti-aging agent. [ABSTRACT FROM AUTHOR]