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Carotenes have been identified as the most essential carotenoids in the human body, together with lycopene, lutein, and β-cryptoxanthin due to their beneficial antioxidant activities. Because of these properties, we have designed and conducted in 2018--2021 an experiment where various tomato accessions were metabolically profiled for their antioxidant content and other fruit parameters for further usage in crop improvement programs. Same genotypes were phenotyped and profiled with DNA markers to characterize their relative genetic distance. Aggregated data from metabolic and genetic profiling were complemented by the phenotypic data to select for the most promising cross (Pl. karotina x L1116) that was performed and developed into F1 and F2 generations. After phenotyping and genetic profiling of the 104 F2 individuals obtained from that cross, 24 individuals were selected for metabolic profiling for antioxidant contents. In relation to carotenoids accumulated in fully matured fruits the highest lycopene concentration detected in our study was 587.03 µg/g DW, while β-carotene reached 440.29 µg/g DW. Our results support the use of such integrative approach to accelerate tomato breeding when improved metabolic content of the fruits is aimed. [ABSTRACT FROM AUTHOR]

The Inter-Simple Sequence Repeat marker technique was applied to a set of tomato genotypes that were found to diverge in antioxidant compounds in fruits. As marker-assisted selection is becoming a tool that brings major advantages for breeding programs need is rising to use it to quickly respond to consumer demand. Being part of a system for effi cient plant breeding in many crops the aim of the present study was to test Inter-Simple Sequence Repeats as candidate tool for incorporating in a breeding program aiming to improve antioxidant properties of tomato fruits. Application of Inter-Simple Sequence Repeat markers resulted in obtaining adequate representation of every accession from the selected group. This opens the opportunity for using the system in confirming the hybrid nature of the F1 progenies and further accelerating the breeding of genotypes for use as healthy food [ABSTRACT FROM AUTHOR]

The Inter-Simple Sequence Repeat marker technique was applied to parental tomato genotypes and their F1 hybrids differing in the antioxidant compounds in the fruits. As the marker-assisted selection is becoming a tool that brings major advantages for breeding programs, the need to use it to quickly respond to the consumer demand has greatly increased. Modern genetic technology opens the possibility for effective application of a system for efficient plant breeding in many crops, including tomatoes. The aim of the present study was to test the Inter-Simple Sequence Repeats as a candidate tool to use in Marker-Assisted Selection in tomatoes. The application of the Inter-Simple Sequence Repeat markers allowed for efficient verification of the hybrid origin of the progeny from parental genotypes that were crossed for improving the antioxidant content in tomato fruits. [ABSTRACT FROM AUTHOR]