1. Phytochemistry and Antioxidant Activity of Aerial Parts of Phagnalon sordidum L.
- Author
-
Cherchar H, Faraone I, DʼAmbola M, Sinisgalli C, Dal Piaz F, Oliva P, Kabouche A, Kabouche Z, Milella L, and Vassallo A
- Subjects
- Antioxidants isolation & purification, Hep G2 Cells drug effects, Humans, Magnetic Resonance Spectroscopy, Oxidative Stress drug effects, Plant Extracts isolation & purification, Antioxidants pharmacology, Asteraceae chemistry, Plant Components, Aerial chemistry, Plant Extracts pharmacology
- Abstract
One new natural monoterpene, 5- O - β -d-glucopyranosyl-2-hydroxy- p -cymene (1: ), and 11 known compounds were isolated through a biologically oriented approach from the aerial parts of Phagnalon sordidum L. The most active extract and fractions were selected using 3 complementary antioxidant activity assays. Results and the different methods were compared by relative antioxidant capacity index. In addition, the most active extract of P. sordidum was subjected to liquid chromatography coupled with electrospray ionization hybrid linear ion trap quadrupole Orbitrap mass spectrometry to quantify secondary metabolites. Antioxidant activities of ethyl acetate extract, and purified 3,4-dihydroxyacetophenone (3: ) and nebrodenside A (7: ) were demonstrated by in vitro cell free model assays, and their protective effect against H
2 O2 -induced oxidative stress in a HepG2 (human hepatocellular carcinoma) cell line was established., Competing Interests: The authors declare that they have no conflict of interest., (Georg Thieme Verlag KG Stuttgart · New York.)- Published
- 2019
- Full Text
- View/download PDF