1. Chemical characterization and pharmacological properties of polysaccharides from Allium roseum leaves: In vitro and in vivo assays.
- Author
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Kherroubi S, Morjen M, Teka N, Mraihi F, Srairi-Abid N, Le Cerf D, Marrakchi N, Majdoub H, Cherif JK, Jebali J, and Ternane R
- Subjects
- Animals, Humans, Rats, Plant Extracts pharmacology, Plant Extracts chemistry, Male, Edema drug therapy, Edema chemically induced, Macrophages drug effects, Macrophages metabolism, THP-1 Cells, Polysaccharides pharmacology, Polysaccharides chemistry, Polysaccharides isolation & purification, Plant Leaves chemistry, Antioxidants pharmacology, Antioxidants chemistry, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents chemistry, Allium chemistry
- Abstract
Allium roseum is amongst the most important wild medicinal plants. It is known for its diverse biological properties, including antioxidant, antibacterial and antidiabetic activities. In this work, the polysaccharides (PARLs) were ultrasonically extracted from Allium roesum leaves then purified and analyzed by several techniques. Chemical composition and GC-MS analysis showed that the obtained polysaccharides were composed mainly of glucose (40.20 %), mannose (25.30 %), fructose (10.60 %) and galacturonic acid (15.11 %). Moreover, PARLs exhibited a potent antioxidant effect with higher capacities up to 69.61 % and 71.72 % for DPPH and ABTS free radicals, respectively. Furthermore, PARLs significantly modulated inflammatory response by reducing TNF-α, IL-6, and IL-8 pro-inflammatory mediators and promoting the anti-inflammatory IL-10 mediator in LPS stimulated THP-1 derived macrophages. The in-vivo tests proved that the extract was able to decrease carrageenan-induced rat paw swelling by around 68.15 % after 4 h of treatment. PARLs, significantly reduced the growth of U87 (glioblastoma) and IGROV-1 cancer cells with IC
50 values of about 4.27 and 7.89 mg/mL respectively. This research clearly shows that Allium roseum polysaccharides can be used as natural antioxidants with anti-inflammatory and anticancer properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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