1. Bioactivity-based analysis and chemical characterization of hypoglycemic and antioxidant components from Artemisia argyi.
- Author
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Xiao JQ, Liu WY, Sun HP, Li W, Koike K, Kikuchi T, Yamada T, Li D, Feng F, and Zhang J
- Subjects
- Animals, Antioxidants chemistry, Antioxidants isolation & purification, Benzothiazoles antagonists & inhibitors, Biphenyl Compounds antagonists & inhibitors, Blood Glucose drug effects, Dose-Response Relationship, Drug, Fluorescence Recovery After Photobleaching, Glycoside Hydrolase Inhibitors chemistry, Glycoside Hydrolase Inhibitors isolation & purification, Hypoglycemic Agents chemistry, Hypoglycemic Agents isolation & purification, Intestine, Small enzymology, Male, Mice, Mice, Inbred ICR, Molecular Docking Simulation, Molecular Structure, Picrates antagonists & inhibitors, Plant Extracts chemistry, Plant Extracts isolation & purification, Structure-Activity Relationship, Sulfonic Acids antagonists & inhibitors, alpha-Glucosidases metabolism, Antioxidants pharmacology, Artemisia chemistry, Glycoside Hydrolase Inhibitors pharmacology, Hypoglycemic Agents pharmacology, Plant Extracts pharmacology
- Abstract
Diabetes is one of the metabolic disorders in the world. It is the prime reason of mortality and morbidity owing to hyperglycemia which is link with numerus obstacles. Artemisia argyi is commonly used as an ingredient in healthy foods as well as an herbal medicine in Asian countries. The present research aims to evaluate the hypoglycemic effects of A. argyi and reveal its the potentially active constituents. The chemical composition was identified by HPLC-DAD-Q-TOF-MS, and fractionation was performed by extraction. The fractions were assessed by the blood glucose level, oral glucose tolerance and small intestinal α-glucosidase inhibitory tests, and an analysis of the total phenolic content (TPC), antioxidant and α-glucosidase inhibitory activities. In our efforts to characterize the compounds responsible for hypoglycemic effect, bioactivity-guided fraction of the MeOH extract and chemical investigation of its active EtOAc fraction led to the successful identification of caffeoylquinic acids, which were elucidated by molecular docking, using the crystal structure of S. cerevisiae isomaltase (PD code: 3AXI). In summary, this bio-guided search revealed that caffeoylquinic acids from A. argyi as potential active constituents displayed with hypoglycemic activity, which provided a basis for further study of pharmacological activity., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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