1. Antioxidant activity against H 2 O 2 -induced cytotoxicity of the ethanol extract and compounds from Pyrola decorate leaves.
- Author
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Yang X, Peng Q, Liu Q, Hu J, Tang Z, Cui L, Lin Z, Xu B, Lu K, Yang F, Sheng Z, Yuan Q, Liu S, Zhang J, and Zhou X
- Subjects
- Animals, Antioxidants analysis, Antioxidants chemistry, Antioxidants isolation & purification, Apoptosis drug effects, Cell Line, Tumor, Cell Survival drug effects, China, Ethanol chemistry, Ethnopharmacology, Hydrogen Peroxide antagonists & inhibitors, Hydrogen Peroxide toxicity, Iridoids analysis, Iridoids chemistry, Iridoids isolation & purification, Iridoids pharmacology, Molecular Structure, Neurons cytology, Neuroprotective Agents analysis, Neuroprotective Agents chemistry, Neuroprotective Agents isolation & purification, Oxidants antagonists & inhibitors, Oxidants toxicity, Plant Extracts chemistry, Plant Extracts isolation & purification, Rats, Solvents chemistry, Triterpenes analysis, Triterpenes chemistry, Triterpenes isolation & purification, Triterpenes pharmacology, Ursolic Acid, Antioxidants pharmacology, Neurons drug effects, Neuroprotective Agents pharmacology, Oxidative Stress drug effects, Plant Extracts pharmacology, Plant Leaves chemistry, Pyrola chemistry
- Abstract
Context: The leaves of Pyrola decorate H. Andr (Pyrolaceae), known as Luxiancao, have long been used for treating kidney deficiency, gastric haemorrhage and rheumatic arthritic diseases in traditional Chinese medicine., Objective: The phytochemicals and antioxidant capacities in vitro of P. decorate leaves were investigated., Materials and Methods: Ethanol, petroleum ether, acetidin, n-butyl alcohol and aqueous extracts of Pyrola decorate leaves were prepared by solvent sequential process, and then isolated and purified to obtain phytochemicals. Cell viability was measured by MTT assay. PC12 cells were pretreated for 24 h with different extractions of P. decorate leaves at concentrations of 0.1, 0.5, 1, 5 and 10 mg/mL, then H
2 O2 of 0.4 mM was added in all samples for an additional 2 h. The antioxidant capacities of betulin, ursolic acid and monotropein were determined in PC12 cells against H2 O2 induced cytotoxicity in vitro as well., Results: Nine compounds (1-9) were isolated and structurally determined by spectroscopic methods, especially 2D NMR analyses. Ethanol extract treated groups showed inhibitory activity with IC50 value of 10.83 mg/mL. Betulin, ursolic acid and monotropein were isolated from P. decorate, and demonstrated with IC50 values of 6.88, 6.15 and 6.13 μg/mL, respectively., Discussion and Conclusions: In conclusion, Pyrola decorate is a potential antioxidative natural plant and worth testing for further pharmacological investigation in the treatment of oxidative stress related neurological disease.- Published
- 2017
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