1. Daily Intake of Grape Powder Prevents the Progression of Kidney Disease in Obese Type 2 Diabetic ZSF1 Rats.
- Author
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Almomen SM, Guan Q, Liang P, Yang K, Sidiqi AM, Levin A, and Du C
- Subjects
- Animals, Antioxidants isolation & purification, Antioxidants metabolism, Biomarkers blood, Biomarkers urine, Cell Line, Crosses, Genetic, Diabetic Nephropathies complications, Diabetic Nephropathies metabolism, Diabetic Nephropathies physiopathology, Disease Progression, Fruit chemistry, Gene Expression Regulation, Kidney metabolism, Kidney pathology, Kidney physiopathology, Male, Metabolic Syndrome complications, Mice, Organ Size, Oxidative Stress, Plant Preparations isolation & purification, Plant Preparations metabolism, Podocytes metabolism, Podocytes pathology, Random Allocation, Rats, Inbred SHR, Rats, Zucker, Renal Insufficiency, Chronic complications, Renal Insufficiency, Chronic metabolism, Renal Insufficiency, Chronic physiopathology, Antioxidants therapeutic use, Diabetes Mellitus, Type 2 complications, Diabetic Nephropathies diet therapy, Dietary Supplements, Plant Preparations therapeutic use, Renal Insufficiency, Chronic diet therapy, Vitis chemistry
- Abstract
Individuals living with metabolic syndrome (MetS) such as diabetes and obesity are at high risk for developing chronic kidney disease (CKD). This study investigated the beneficial effect of whole grape powder (WGP) diet on MetS-associated CKD. Obese diabetic ZSF1 rats, a kidney disease model with MetS, were fed WGP (5%, w / w ) diet for six months. Kidney disease was determined using blood and urine chemical analyses, and histology. When compared to Vehicle controls, WGP intake did not change the rat bodyweight, but lowered their kidney, liver and spleen weight, which were in parallel with the lower serum glucose and the higher albumin or albumin/globin ratio. More importantly, WGP intake improved the renal function as urination and proteinuria decreased, or it prevented kidney tissue damage in these diabetic rats. The renal protection of WGP diet was associated with up-regulation of antioxidants ( Dhcr24 , Gstk1 , Prdx2 , Sod2 , Gpx1 and Gpx4 ) and downregulation of Txnip (for ROS production) in the kidneys. Furthermore, addition of grape extract reduced Hâ‚‚Oâ‚‚-induced cell death of cultured podocytes. In conclusion, daily intake of WGP reduces the progression of kidney disease in obese diabetic rats, suggesting a protective function of antioxidant-rich grape diet against CKD in the setting of MetS.
- Published
- 2017
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