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Synthetic phenolic antioxidants (SPAs) are widely used in diverse industries due to their exceptional antioxidant characteristics. However, human exposure to SPAs may cause health problems. In this study, 226 dust samples were collected from 10 provinces in China, and six SPAs (three parent SPAs and their three transformation products) were analyzed. The concentrations of ∑ 6 SPAs (the sum of six target compounds) ranged from 15.4 to 3210 ng/g (geometric mean (GM): 169 ng/g). The highest concentration of ∑ 6 SPAs was found in Sichuan Province (GM: 349 ng/g), which was approximately 4 times higher than that in Hubei Province (81.6 ng/g) (p < 0.05). The concentrations of butylated hydroxytoluene (BHT), 2,2'-methylene bis (4-methyl-6‑tert-butylphenol) (AO2246), 2,6-di‑tert‑butyl‑1,4-benzoquinone (BHT-Q), 2,6-di‑tert‑butyl‑4-(hydroxymethyl) phenol (BHT-OH), and ∑p-SPAs were substantially higher in dust from urban areas than rural areas (p < 0.05). AO2246 concentration in dust from homes (GM: 0.400 ng/g) was about 4 times higher than that in workplaces (0.116 ng/g) (p < 0.01). Significantly higher ∑p-SPAs concentrations were found in dust from homes (GM: 17.5 ng/g) than workplaces (11.4 ng/g) (p < 0.01). The estimated daily intakes (EDIs) of ∑ 6 SPAs exposed through dust ingestion were 0.582, 0.342, 0.197, 0.076, and 0.080 ng/kg bw/day in different age groups, and exposed through dermal contact was 0.358, 0.252, 0.174, 0.167, and 0.177 ng/kg bw/day. EDIs showed that the exposure risks of SPAs decreased with age. This is the first work to determine SPAs in dust from 10 provinces in China and investigate the spatial distribution of SPAs in those regions., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Synthetic phenolic antioxidants (SPAs) are widely used as food additives to delay the oxidation rate of oils and oil products. The concentrations and compositions of SPAs in Chinese residents' most popular daily foods and the resulting exposure risk of SPAs are not clear. Therefore, this study collected food samples in 13 food categories (n = 289) from 10 provinces in China. At least one of the SPAs was detected in approximately 99.7% of foodstuffs, and the concentration of ∑SPAs ranged from not detected to 7830 (geometric mean (GM): 296 ng/g wet weight). 2,6-2 tert butyl p-1,4-benzoquinone (BHT-Q) was the main transformation product, but SPAs in food tend to exist in the parent form. A significant difference between food categories was analyzed. The highest GM of ΣSPAs occurred in cereals and cereal products, which was approximately 8 times higher than that in beverages. The estimated daily intakes of ΣSPAs in Chinese preschoolers, school-age children, adults and elderly individuals were 22200, 9970, 7540 and 7700 ng/kg bw/day, respectively. The exposure risks of SPAs decreased with age. This is the first simultaneous analysis of SPAs in multiple provinces and multiple categories of foodstuffs., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The continuous improvement of living standards is related to higher requirements for the freshness and taste of food. For example, synthetic phenolic antioxidants (SPAs) are added to fats and fried foods as food additives to minimize the oxidative rancidity of oils and fats. Hence, the global use of SPAs is increasing year by year. Dibutyl hydroxytoluene is one of the widely used SPAs, often in combination with butyl hydroxyanisole or gallate SPAs. The extensive use of these compounds makes them and their transformation products to be widespread in various environmental matrices, including indoor dust, wastewater, river water, sewage sludge, and sediment, as well as human samples, such as nails and urine, at concentrations varying from nanogram per gram (ng/g) to microgram per gram (μg/g). Animal experiments have shown that high-dose SPA exposure is toxic, which may lead to DNA damage and mismatches and the development of cancerous tumors. Since the biosphere shares the same set of genetic codes, humans and animals have many identical or similar feedback mechanisms and information pathways. Therefore, the damage of SPAs to animals may also threaten human health. This review discusses the properties, occurrence, analysis, and environmental health risks of typical SPAs, including butyl hydroxyanisole, dibutyl hydroxytoluene, tert-butylhydroquinone, propyl gallate, octyl gallate, and lauryl gallate, used as food additives. In addition, AO2246, which is used in food packaging bags, is also considered. Future research directions on SPAs and their transformation products (TPs) are identified and discussed., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Synthetic phenolic antioxidants (SPAs) have gained high concerns due to their extensive usages and unintended environmental release via various routes. Their contamination in water system could pose potential threat to aquatic organisms, therefore, the studies on the aquatic toxicology of this kind of chemicals are of high importance. In this research, the developmental toxicities of four commonly used SPAs, including butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ), and 2,2'-methylenebis (6-tert-butyl-4-methylphenol) (AO2246) were investigated using the zebrafish embryo toxicity test (ZFET). The results showed that these four SPAs exerted different acute toxicities to zebrafish, and the toxic order, based on their 96 h LC 50 values, was AO2246 > TBHQ > BHA > BHT, and decreased hatching rates were induced for the embryos in BHA, TBHQ and AO2246 exposure groups. Non-lethal exposures of BHA (≤20 μM), TBHQ (≤20 μM), BHT (≤200 μM) and AO2246 (≤2 μM) decreased the heart rates and body lengths of zebrafish in exposure concentration-dependent manners. Diverse morphological deformities, including uninflated swim bladder, pericardial edema, spinal curvature, severe yolk deformation, or abnormal pigmentation, were induced in zebrafish larvae upon SPA treatments. The transcriptional levels of the related genes, examined by quantitative PCR, indicated that the interferences of SPAs with hypothalamic-pituitary-thyroid axis (HPT axis), GH/PRL synthesis and Hedgehog (hh) pathway contributed to their developmental toxicities in zebrafish. The up-regulation of pluripotency biomarker, Oct4, caused the developmental retardation during the early stages of zebrafish embryos in BHA and TBHQ exposure groups. The results obtained herein provided important information on the developmental toxicity of SPAs, which could be very helpful in guiding the risk assessment on their aquatic toxicology., (Copyright © 2018 Elsevier B.V. All rights reserved.)