1. Hepatoprotective and anti-oxidant activities of Glossogyne tenuifolia against acetaminophen-induced hepatotoxicity in mice.
- Author
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Tien YH, Chen BH, Wang Hsu GS, Lin WT, Huang JH, and Lu YF
- Subjects
- Alanine Transaminase blood, Animals, Aspartate Aminotransferases blood, Biomarkers blood, Chemical and Drug Induced Liver Injury diagnosis, Dose-Response Relationship, Drug, Drug Overdose, Glutathione metabolism, Glutathione Disulfide metabolism, Lipid Peroxidation drug effects, Liver metabolism, Male, Mice, Inbred BALB C, Plant Extracts chemistry, Acetaminophen poisoning, Analgesics, Non-Narcotic poisoning, Antioxidants, Antipyretics poisoning, Asteraceae chemistry, Chemical and Drug Induced Liver Injury drug therapy, Phytotherapy, Plant Extracts pharmacology, Plant Extracts therapeutic use
- Abstract
The present study investigated the anti-oxidative and hepatoprotective effects of Glossogyne tenuifolia (GT) Cassini, against acetaminophen-induced acute liver injury in BALB/c mice. The extracts of GT by various solvents (hot water, 50% ethanol and 95% ethanol) were compared for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, total phenolic content, and total anti-oxidant capacity. The results showed that hot water (HW) extracts of GT contained high levels of phenolics and exerted an excellent anti-oxidative capacity; thus, these were used in the animal experiment. The male BALB/c mice were randomly divided into control group, acetaminophen (APAP) group, positive control group and two GT groups at low (GT-L) and high (GT-H) dosages. The results showed that mice treated with GT had significantly decreased serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). GT-H increased glutathione levels and the ratios of reduced glutathione and oxidized glutathione (GSH/GSSG) in the liver, and inhibited serum and lipid peroxidation. This experiment was the first to determine phenolic compounds, chlorogenic acid and luteolin-7-glucoside in HW extract of GT. In conclusion, HW extract of GT may have potential anti-oxidant capacity and show hepatoprotective capacities in APAP-induced liver damaged mice.
- Published
- 2014
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