1. Response surface optimization of microfluidic formulations of nanobilosomes for enhancement of aqueous solubility, digestive stability, and cellular antioxidant activity of mangiferin.
- Author
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Thiengkaew P, Thanitwatthanasak S, Srisala S, Jittorntrum B, Chunhabundit R, and Chitprasert P
- Subjects
- Antioxidants metabolism, Caco-2 Cells, Digestion, Drug Compounding, Humans, Lab-On-A-Chip Devices, Particle Size, Solubility, Xanthones metabolism, Antioxidants chemistry, Antioxidants pharmacology, Nanostructures chemistry, Water chemistry, Xanthones chemistry, Xanthones pharmacology
- Abstract
Mangiferin-loaded nanobilosomes (MGF-NBSs) were developed using microfluidic-based techniques to improve aqueous solubility, digestive stability, and cellular antioxidant activity (CAA) of mangiferin. Preliminary experiments showed that optimal formation conditions were 5:1 aqueous (water) to solvent (ethanol) phase ratio and 85 mL/min total flow rate. Further optimization using response surface methodology provided the optimal formulation (200 mg encapsulant consisting of 90.91% phosphatidylcholine and 9.09% sodium glycocholate, and 25.89 mg mangiferin), achieving 9.25% mangiferin loading and 80.65% encapsulation efficiency. Mono-dispersed MGF-NBSs with an average size of around 48.14 nm and zeta potential of -30.1 mV were obtained. FTIR and DSC results confirmed the successful encapsulation of mangiferin into the nanobilosomes and revealed interactions among the components. MGF-NBSs showed a 7-fold increase in the aqueous solubility compared with non-encapsulated mangiferin. CAA of MGF-NBSs in Caco-2 cells was 2 times higher than that of mangiferin and the in vitro digestive stability was improved., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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