1. Not all 2',4'-bridged modifications stabilize DNA/RNA duplexes.
- Author
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Tomoka Akita, Tomita-Sudo, Elisa, Shin Itoh, Nae Sakimoto, Takeshi Masuda, Akifumi Nakamura, Yoshiyuki Onishi, Makoto Koizumi, and Junji Kawakami
- Subjects
RNA ,DNA ,NUCLEOTIDES - Abstract
2',4'-Bridged modifications such as 2'-O,4'-C-methylene-bridged nucleotides (LNAs) and 2'-O,4'-C-ethylene-bridged nucleotides (ENAs) provide high binding affinity for duplex formation. Stabilization by the introduction of the bridged nucleic acids is considered to be due to pre-organization. In this study, we found that the introduction of 2',4'-C-bridged 2'-deoxynucleotides (CRNs; Conformationally Restricted Nucleotides) into DNA/RNA duplexes leads to destabilization, as opposed to the previously accepted notion that 2',4'-bridged modifications always lead to stabilization. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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