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We determined the complete mitochondrial genome of Kaloula rugifera in this work. The mitogenome was 17,073 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (D-loop). The base composition of the light strand was 29.7% A, 30.4% T, 25.7% C and 14.2% G. The gene order and contents of it is identical to most amphibian mitogenome. All protein-coding genes began with ATG as start codon except ND1 gene beginning with GTG and COI gene beginning with ATA. Five protein-coding genes (COII, ATP 6, COIII, ND3 and ND4) ended with incomplete stop codon T. The 22 tRNA genes with the size ranging from 65 bp to 73 bp were interspersed along the whole genome. The D-loop region containing tandem repetition was 1675 bp in length and heavily biased to A + T nucleotides.

The complete mitochondrial genome of a stream-dwelling frog species Paramegophrys oshanensis was determined. This mitogenome was 17,747 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region (D-loop). The base composition of the light strand was 28.77% A, 29.85% T, 26.26% C, and 15.11% G. Compared with most other vertebrates, this mitogenome has some different features: a non-tandem duplication of tRNA-Met gene (forming tRNA-Met1 and tRNA-Met2 genes), the replacement of tRNA-Trp gene by a 72-bp noncoding region translocated from the cluster of WANCY to the back of Cytb gene, the translocation of tRNA-Val gene from the front of 16S rRNA to the back of tRNA-Met1 gene, and the translocation of tRNA-Pro gene from the front of D-loop region to the front of tRNA-Met2 gene. Three kinds of tandem repeats with size ranging from 71 to 92 bp were detected within D-loop region.

The complete mitochondrial genome of the Leptobrachella alpina Fei, Ye, and Li 1990, was assembled for the first time. The mitogenome of this species was 17,763 bp in length, containing 13 protein-coding genes, two ribosomal RNA genes (12S rRNA and 16S rRNA), 22 transfer RNA genes (tRNA), and a non-coding control region (D-loop). The base content of the mitogenome was that A, T, G, and C occupied 28.5%, 30.8%, 15.1%, and 25.6%, respectively. The phylogenetic analysis was conducted based on 17 complete mitogenome sequences of the family Megophryidae by the Bayesian inference approach. The phylogenetic tree suggested that Leptobrachium and Oreolalax clustered into a clade and formed a sister group with Leptobrachella. This work is critical for the further genetic research and conservation of this species. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome of theOreolalax lichuanensiswas determined. It is a circular molecule of 17 702 bp in size and consists of 13 protein-coding genes, 23 tRNA genes, two rRNA genes and a control region (D-loop). The base composition on light strand was 28.0% A, 32.2% T, 24.9% C and 14.9% G. Compared with most other vertebrates, this mitogenome appear a tandem duplication oftRNA-Metgene. This study will facilitate the further research of the population genetics of this species and systematic study of the genusOreolalax. [ABSTRACT FROM PUBLISHER]