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Muhammad Junaid Tahir,1 Waleed Tariq,1 Muhammad Anas Tahseen Asar,1 Muhammad Irfan Malik,1,2 Fahd Kamal Akhtar,1 Muna Malik,3 Qirat Akhtar,4 Khabab Abbasher Hussien Mohamed Ahmed,5 Muhammad Talha Awan,1 Kaleem Ullah,6 Muhammad Sohaib Asghar7 1Lahore General Hospital, Lahore, Pakistan; 2Postgraduate Medical Institute, Lahore, Pakistan; 3Combined Military Hospital, Lahore, Pakistan; 4Army Medical College, Rawalpindi, Pakistan; 5University of Khartoum, Faculty of Medicine, Khartoum, Sudan; 6Pir Abdul Qadir Shah Jeelani Institute of Medical Sciences, Gambat, Sindh, Pakistan; 7Dow University of Health Sciences–Ojha Campus, Karachi, PakistanCorrespondence: Khabab Abbasher Hussien Mohamed Ahmed, Faculty of Medicine, University of Khartoum, Khartoum, 11111, Sudan, Email Khabab9722@gmail.comBackground: This study was carried out to find the awareness level, prevalence of anxiety and depression, and their associated factors among doctors and medical students during the COVID-19 pandemic in Punjab province, Pakistan.Materials and Methods: An online questionnaire-based cross-sectional study was conducted; data were collected regarding the demographic profile, awareness of COVID-19, anxiety symptoms using a self-rating anxiety scale (SAS), and depression symptoms using a self-rating depression scale (SDS). A total of 504 responses were obtained. The Chi-square test was used to compare groups and the logistic regression model for the association of factors with anxiety and depression.Results: A total of 504 medical students and doctors responded to the questionnaire. The mean age of participants was 25.66 ± 6.71 years. The awareness rate of COVID-19 was 97.6%. The prevalence rate of anxiety and depression was 3.4% and 15.1%, respectively. According to the logistic regression model, those with low awareness levels (≤ 6 points) and spending more time on the news (> 3 hours) had higher levels of anxiety, while higher levels of depression symptoms were reported in females, young people, and medical students.Conclusion: Doctors and medical students had a high level of awareness of COVID-19 and a low level of anxiety and depression. However, female gender and younger age group were the most vulnerable groups significantly associated with depression.Keywords: COVID-19, doctors, medical students, awareness, anxiety, depression, infection