30 results on '"Guo, Xingqi"'
Search Results
2. Identification of an Apis cerana cerana MAP kinase phosphatase 3 gene (AccMKP3) in response to environmental stress
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Chao, Yuzhen, Wang, Chen, Jia, Haihong, Zhai, Na, Wang, Hongfang, Xu, Baohua, Li, Han, and Guo, Xingqi
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- 2019
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3. Characteristics of AccSTIP1 in Apis cerana cerana and its role during oxidative stress responses
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Zhai, Na, Jia, Haihong, Ma, Manli, Chao, Yuzhen, Guo, Xingqi, and Li, Han
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- 2018
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4. The gene AccCyclin H mitigates oxidative stress by influencing trehalose metabolism in Apis cerana cerana.
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Peng, Hongyan, Guo, Dezheng, Peng, Hongmei, Guo, Hengjun, Wang, Hongfang, Wang, Ying, Xu, Baohua, Gao, Aiying, Liu, Zhenguo, and Guo, Xingqi
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TREHALOSE ,APIS cerana ,OXIDATIVE stress ,OXIDANT status ,GENE silencing ,RNA interference ,METABOLISM - Abstract
BACKGROUND: Environmental stress can induce oxidative stress in Apis cerana cerana, leading to cellular oxidative damage, reduced vitality, and even death. Currently, owing to an incomplete understanding of the molecular mechanisms by which A. cerana cerana resists oxidative damage, there is no available method to mitigate the risk of this type of damage. Cyclin plays an important role in cell stress resistance. The aim of this study was to explore the in vivo protection of cyclin H against oxidative damage induced by abiotic stress in A. cerana cerana and clarify the mechanism of action. We isolated and identified the AccCyclin H gene in A. cerana cerana and analysed its responses to different exogenous stresses. RESULTS: The results showed that different oxidative stressors can induce or inhibit the expression of AccCyclin H. After RNA‐interference‐mediated AccCyclin H silencing, the activity of antioxidant‐related genes and related enzymes was inhibited, and trehalose metabolism was reduced. AccCyclin H gene silencing reduced A. cerana cerana high‐temperature tolerance. Exogenous trehalose supplementation enhanced the total antioxidant capacity of A. cerana cerana, reduced the accumulation of oxidants, and improved the viability of A. cerana cerana under high‐temperature stress. CONCLUSION: Our findings suggest that trehalose can alleviate adverse stress and that AccCyclin H may participate in oxidative stress reactions by regulating trehalose metabolism. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
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- 2024
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5. AccsHSP21.7 enhances the antioxidant capacity of Apis cerana cerana.
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Huang, Yuanyuan, Zhang, Yuanying, Niu, Xiaojing, Sun, Yunhao, Wang, Hongfang, Guo, Xingqi, Xu, Baohua, and Wang, Chen
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APIS cerana ,OXIDANT status ,HEAT shock proteins ,OXIDATIVE stress ,RNA interference ,GLYPHOSATE - Abstract
BACKGROUND: The widespread use of glyphosate has many adverse effects on Apis cerana cerana. Due to the incomplete understanding of the molecular mechanisms of glyphosate toxicity, there are no available methods for mitigating the threat of glyphosate to Apis cerana cerana. Small heat shock proteins (sHSPs) play an important role in resisting oxidative stress, but their mechanism of action in Apis cerana cerana remains unclear. RESULTS: In this experiment, we cloned and identified AccsHSP21.7. Studies have shown that AccsHSP21.7 contains binding motifs for various transcription factors related to oxidative stress. Abiotic stresses induced the expression of AccsHSP21.7. Bacteriostatic testing of a recombinant AccsHSP21.7 protein proved that Escherichia coli overexpressing AccsHSP21.7 showed increased resistance to oxidative stress. Knocking down the AccsHSP21.7 gene caused significant damage to midgut cells, which seriously disrupted the antioxidant system in Apis cerana cerana and greatly increased mortality under glyphosate stress. CONCLUSION: This study investigated the relationship between antioxidant regulation and the AccsHSP21.7 gene at the molecular level, and the results have guiding significance for the improvement of stress resistance in Apis cerana cerana. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Characterization of the CDK5 gene in Apis cerana cerana (AccCDK5) and a preliminary identification of its activator gene, AccCDK5r1
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Zhao, Guangdong, Wang, Chen, Wang, Hongfang, Gao, Lijun, Liu, Zhenguo, Xu, Baohua, and Guo, Xingqi
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- 2017
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7. Molecular cloning, expression, and stress response of the estrogen-related receptor gene (AccERR) from Apis cerana cerana
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Zhang, Weixing, Zhu, Ming, Zhang, Ge, Liu, Feng, Wang, Hongfang, Guo, Xingqi, and Xu, Baohua
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- 2016
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8. A novel 1-Cys thioredoxin peroxidase gene in Apis cerana cerana: characterization of AccTpx4 and its role in oxidative stresses
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Huaxia, Yifeng, Wang, Fang, Yan, Yan, Liu, Feng, Wang, Hongfang, Guo, Xingqi, and Xu, Baohua
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- 2015
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9. A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress
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Zhang, Yuanying, Yan, Huiru, Lu, Wenjing, Li, Yuzhen, Guo, Xingqi, and Xu, Baohua
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- 2013
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10. Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana
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Yan, Huiru, Jia, Haihong, Gao, Hongru, Guo, Xingqi, and Xu, Baohua
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- 2013
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11. Identification and characterization of an Apis cerana cerana Delta class glutathione S-transferase gene (AccGSTD) in response to thermal stress
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Yan, Huiru, Jia, Haihong, Wang, Xiuling, Gao, Hongru, Guo, Xingqi, and Xu, Baohua
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- 2013
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12. Identification and antioxidant characterisation of thioredoxin-like1 from Apis cerana cerana
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Lu, Wenjing, Kang, Mingjiang, Liu, Xiufang, Zhao, Xiaocui, Guo, Xingqi, and Xu, Baohua
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- 2012
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13. Molecular Mechanism of the UDP-Glucuronosyltransferase 2B20-like Gene (AccUGT2B20-like) in Pesticide Resistance of Apis cerana cerana.
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Cui, Xuepei, Wang, Chen, Wang, Xinxin, Li, Guilin, Liu, Zhenguo, Wang, Hongfang, Guo, Xingqi, and Xu, Baohua
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PESTICIDE resistance ,APIS cerana ,GLUCURONOSYLTRANSFERASE ,GENE silencing ,OXIDATIVE stress - Abstract
UDP-glucuronosyltransferases (UGTs), being multifunctional detoxification enzymes, play a major role in the process of resistance to various pesticides in insects. However, the mechanism underlying the molecular regulation of pesticide resistance remains unclear, especially in Apis cerana cerana. In this study, all of the UGTs in Apis cerana cerana (AccUGT) have been identified through the multiple alignment and phylogenetic analysis. Expression of AccUGT genes under different pesticides, and antioxidant genes after silencing of AccUGT2B20-like , were detected by qRT-PCR. The resistance of overexpressed AccUGT2B20-like to oxidative stress was investigated by an Escherichia coli overexpression system. Also, antioxidant-related enzyme activity was detected after silencing of the AccUGT2B20-like gene. Expression pattern analysis showed that almost all UGT genes were upregulated under different pesticide treatments. This result indicated that AccUGTs participate in the detoxification process of pesticides. AccUGT2B20-like was the major gene because it was more highly induced than the others. Overexpression of AccUGT2B20-like in E. coli could effectively improve oxidative stress resistance. Specifically, silencing the AccUGT2B20-like gene increased oxidative stress by repressing the expression of oxidation-related genes, decreasing antioxidant-related enzyme activity, and increasing malondialdehyde concentration. Taken together, our results indicate that AccUGTs are involved in pesticide resistance, among which, AccUGT2B20-like contributes to the detoxification of pesticides by eliminating oxidative stress in Apis cerana cerana. This study explains the molecular basis for the resistance of bees to pesticides and provides an important safeguard for maintaining ecological balance. [ABSTRACT FROM AUTHOR]
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- 2020
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14. Role of Apis cerana cerana N-terminal asparagine amidohydrolase (AccNtan1) in oxidative stress.
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Zhao, Guangdong, Wang, Chen, Wang, Ying, Wang, Lijun, Xu, Baohua, and Guo, Xingqi
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APIS cerana ,PUPAE ,OXIDATIVE stress ,DNA repair ,POLYMERASE chain reaction ,PROTEOLYSIS - Abstract
N-Terminal asparagine amidohydrolase is a component of the ubiquitin-dependent N-end rule pathway of protein degradation that has been implicated in a variety of physiological functions, including the sensing of heme, oxygen, nitric oxide, selective elimination of misfolded proteins and the repair of DNA. We identified the Apis cerana cerana N-terminal asparagine amidohydrolase (AccNtan1) gene from A. cerana cerana and investigated its role in oxidation resistance. Multiple sequence alignments and phylogenetic analysis revealed that N-terminal asparagine amidohydrolase is highly conserved in insect species. Quantitative real-time polymerase chain reaction analysis indicated that the expression levels of AccNtan1 were significantly lower in the wing, honey sac and abdomen than in other tissues and were significantly higher in early stages of development, including the larva, prepupa and pink-eyed pupa stages, than in later stages. We further observed that AccNtan1 expression was induced by several environmental stressors, including aberrant temperature, H
2 O2 , UV, heavy metals and pesticides. Moreover, a bacteriostatic assay suggested that overexpression of AccNtan1 enhances the resistance of bacteria to oxidative stress. In addition, knockdown of AccNtan1 using RNA interference significantly affected the expression levels of most antioxidant genes and the activity levels of several antioxidant enzymes. Thus, we hypothesize that AccNtan1 plays important roles in environmental stress responses and antioxidative processes. [ABSTRACT FROM AUTHOR]- Published
- 2020
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15. Identification and Characterization of a Novel Methionine Sulfoxide Reductase B Gene (AccMsrB) from Apis cerana cerana (Hymenoptera: Apidae)
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Liu, Feng, Gong, Zhihong, Zhang, Weixing, Wang, Ying, Ma, Lanting, Wang, Hongfang, Guo, Xingqi, and Xu, Baohua
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- 2015
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16. Cloning, structural features, and expression analysis of the gene encoding thioredoxin reductase 1 from Apis cerana cerana
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Yang, Haifang, Kang, Mingjiang, Guo, Xingqi, and Xu, Baohua
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MOLECULAR cloning , *MOLECULAR structure , *GENE expression , *THIOREDOXIN , *APIS cerana , *GENETIC code , *OXIDATIVE stress , *POLYMERASE chain reaction - Abstract
Abstract: Previous studies have demonstrated that mammalian thioredoxin reductases 1 (TrxRs; EC 1.6.4.5) play roles in protection against oxidant injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form. However, little is known about the function of TrxRs in insects, especially in Apis cerana cerana. To gain a better understanding of its role in insects, we cloned TrxR1 from A. cerana cerana (AccTrxR1) and investigated its structural features and expression. The full-length cDNA is 1998bp long and contains an open reading frame (ORF) of 1485bp. The deduced AccTrxR1 protein has 494 aa, a calculated molecular mass (MW) of 54.24kDa, and an isoelectric point of pH 7.35. The full-length genomic DNA of AccTrxR1 is 3500bp and contains eight exons and seven introns. In addition, the sequence and putative transcription factor-binding sites of the 5′-flanking region were examined. We also looked for conserved domains/motifs, generated homologous alignments, conducted phylogenetic analysis, and made secondary and tertiary structure predictions using web software. RT-PCR revealed that the expression of AccTrxR1 could be induced by UV and heat (37°C). These results indicate that AccTrxR1 may play a key role in protection against oxidant stress. [Copyright &y& Elsevier]
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- 2010
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17. Functional analysis of AccCDK2-like and AccCINP-like genes in Apis cerana cerana under pesticide and heavy metal stress.
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Li, Jing, Guo, Dezheng, Wang, Fang, Tian, Ming, Wang, Chen, Guo, Xingqi, Xu, Baohua, and Wang, Ying
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APIS cerana , *HEAVY metals , *PESTICIDE resistance , *FUNCTIONAL analysis , *ESCHERICHIA coli , *PESTICIDES - Abstract
Heavy metals and pesticides represent prominent sources of pollution in the natural habitat of Apis cerana cerana , potentially endangering their health through the induction of oxidative stress reactions. This study aimed to address this issue by isolating AccCDK2-like and AccCINP-like proteins from Apis cerana cerana and investigating their functional roles in honey bee resistance against pesticide and heavy metal stresses. Bioinformatics analysis revealed significant homology of these proteins with those found in other species. Functional studies confirmed their participation in interaction with each other, alongside demonstrating distinct patterns of expression and localization. Specifically, AccCDK2-like exhibited higher expression levels in prepupae and muscle tissues, while AccCINP-like showed maximal expression in brown pupae and abdomen. Furthermore, the expression levels of these proteins were found to be modulated in response to pesticide and heavy metal stresses. Notably, overexpression of AccCDK2-like and AccCINP-like led to a noticeable alteration in E. coli 's ability to withstand external stresses. Additionally, silencing of the AccCDK2-like and AccCINP-like genes resulted in a significant reduction in antioxidant enzyme activity and the expression levels of genes related to antioxidant function. Consequently, the mortality rate of Apis cerana cerana under pesticide and heavy metal stresses conspicuously increased. Hence, our findings suggest that AccCDK2-like and AccCINP-like proteins potentially play a crucial role in the response of Apis cerana ceran a to pesticide and heavy metal stress, likely by modulating the antioxidant pathway. [Display omitted] • CDK2-like and CINP-like are involved in Apis cerana pesticide resistance. • CDK2-like and CINP-like are involved in Apis cerana heavy metal resistance. • CDK2-like and CINP-like take part in the Apis cerana 's oxidative stress response. [ABSTRACT FROM AUTHOR]
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- 2023
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18. Identification of the AccCDK7 and AccCDK9 genes and their involvement in the response to resist external stress in Apis cerana cerana.
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Peng, Hongyan, Guo, Dezheng, Shan, Wenlu, Tan, Shuai, Wang, Chen, Wang, Hongfang, Liu, Zhenguo, Xu, Baohua, Guo, Xingqi, and Wang, Ying
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APIS cerana , *CELL cycle regulation , *RNA interference , *ESCHERICHIA coli , *CYCLIN-dependent kinases - Abstract
Previous studies examining the functions of cyclin-dependent kinases (CDKs) have mainly focused on the regulation of the cell cycle. Recent studies have found that cyclin-dependent kinase 7 (CDK7) and cyclin-dependent kinase 9 (CDK9) play important roles in cell stress, metabolism of toxic substances and maintaining the stability of the internal environment. Here, we found that under stress conditions, the transcription and protein expression of AccCDK7 and AccCDK9 were induced to varying degrees. Meanwhile, the silencing of AccCDK7 and AccCDK9 also affected the expression of antioxidant genes and the activity of antioxidant enzymes, and reduced the survival rate of bees under high temperature stress. Furthermore, the exogenous overexpression of AccCDK7 and AccCDK9 improved the viability of yeast under stress conditions. Therefore, AccCDK7 and AccCDK9 may play roles in A.cerana cerana resistance to oxidative stress caused by external stimuli, potentially revealing a new mechanism of the honeybee response to oxidative stress. • Isolation of AccCDK7 and AccCDK9 from Apis cerana cerana. • The expression of AccCDK7 and AccCDK9 is induced by oxidative stress. • AccCDK7 and AccCDK9 can enhance the stress resistance of E. coli and yeast. • RNA interference experiments suggested that AccCDK7 and AccCDK9 is important for resistance against stresses. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Roles of a mitochondrial AccSCO2 gene from Apis cerana cerana in oxidative stress responses.
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Jia, Haihong, Ma, Manli, Zhai, Na, Liu, Zhenguo, Wang, Hongfang, Guo, Xingqi, and Xu, Baohua
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APIS cerana , *CYTOCHROME oxidase , *OXIDATIVE stress , *RNA interference , *SUPEROXIDE dismutase genetics - Abstract
In eukaryotes, cytochrome c oxidase (COX) is a multimeric protein complex that is the last enzyme in the respiratory electron transport chain of mitochondria. Syntheses of cytochrome c oxidase (SCO) proteins are copper-donor chaperones involved in metalation of the CuA redox center of COX. However, its other precise actions are not yet understood. Here, we report the characterization of AccSCO2 from Apis cerana cerana ( Acc ). Our data showed that AccSCO2 expression was induced by cold (4 °C), CdCl 2 , HgCl 2 , ultraviolet (UV) light, and H 2 O 2 and was inhibited by different pesticide treatments. In addition, a disc diffusion assay of recombinant AccSCO2, AccSCO2-R1, and AccSCO2-R2 proteins showed that they played a functional role in protecting cells from oxidative stress involved in copper-dependent manner. Further, following knockdown of AccSCO2 in A. cerana cerana using RNA interference (RNAi), the expression levels of most antioxidant genes ( AccGSTD , AccGSTO1 , AccGSTS4 , AccSOD1 , AccSOD2 , etc.) were significantly decreased in the AccSCO2 -silenced bees compared with the control bees. Moreover, the antioxidant enzymatic activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were all lower in the silenced bees than in the control bees. Finally, the in vivo activity of COX was measured after AccSCO2 knockdown, which revealed a strong reduction in COX activity in the silenced bees. Thus, we hypothesize that AccSCO2 plays important roles in cellular stress responses and anti-oxidative processes, which help to regulate the production of mitochondrial reactive oxygen species and/or the impairment of mitochondrial activity under oxidative stress. [ABSTRACT FROM AUTHOR]
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- 2017
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20. Role of the tyrosine aminotransferase AccTATN gene in the response to pesticide and heavy metal stress in Apis cerana cerana.
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Sun, Yunhao, Niu, Xiaojing, Huang, Yuanyuan, Wang, Lijun, Liu, Zhenguo, Guo, Xingqi, Xu, Baohua, and Wang, Chen
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APIS cerana , *HEAVY metals , *PESTICIDE resistance , *RNA interference , *TYROSINE , *HEAVY metal toxicology , *PESTICIDES , *GLYPHOSATE - Abstract
Tyrosine aminotransferase (TATN) is the first enzyme involved in the metabolic degradation of tyrosine, and it plays an important role in tyrosine detoxification and helps the body resist oxidative damage. However, the function of TATN in Apis cerana cerana (A. c. cerana) remains unclear. To explore the role of TATN in the response to pesticide and heavy metal stress in A. c. cerana , AccTATN was isolated and identified. AccTATN was highly expressed in the integument and the adult stage. Exposure to multiple pesticides and heavy metal stress upregulated AccTATN expression. RNA interference experiments showed that silencing AccTATN reduced the resistance of A. c. cerana to glyphosate and avermectins stress. The expression of antioxidant-related genes and the activity of antioxidant enzymes were reduced after AccTATN was silenced, leading to the accumulation of oxidative damage. Overexpression of the recombinant AccTATN protein in a prokaryotic system also confirmed its role in heavy metal stress and improved antioxidant capacity. Our study showed that AccTATN may promote resistance to pesticide and heavy metal stress by regulating the antioxidant capacity of A. c. cerana. This study provides a valuable theoretical basis for A. c. cerana conservation. [Display omitted] • AccTATN gene was isolated and characterized from (A. c. cerana) • Pesticide and heavy metal stress can affect the expression of AccTATN. • AccTATN overexpression enhanced the tolerance of E. coli cells to oxidative stress. • AccTATN knockdown disrupted the original redox balance and caused oxidative stress. • AccTATN promotes A. c. cerana to resist pesticide and heavy metal stress. [ABSTRACT FROM AUTHOR]
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- 2023
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21. Activating transcription factor 2 (AccATF2) regulates tolerance to oxidative stress in Apis cerana cerana.
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Wang, Lijun, Wang, Jiayu, Guo, Huijuan, Wang, Ying, Xu, Baohua, Guo, Xingqi, and Wang, Chen
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APIS cerana , *OXIDATIVE stress , *TRANSCRIPTION factors , *PUPAE , *PROTHROMBIN , *ESCHERICHIA coli , *BEE colonies - Abstract
Activating transcription factor 2 (ATF2), a basic leucine zipper (bZIP) transcription factor, plays a crucial role in immune and DNA damage response in mammals. However, the function of ATF2 in insects remains unknown. Here, we isolated the ATF2 gene from Apis cerana cerana (AccATF2) and found that AccATF2 was a main regulator of the honeybee response to oxidative stress. Our results showed that AccATF2 was highly expressed in the head, thorax and integument. AccATF2 was expressed throughout the development period of honeybees, and the highest AccATF2 transcript level was noted in brown-eyed pupae, indicating its indispensable roles in honeybee survival. Antioxidant function analysis showed that AccATF2 expression was markedly induced in response to oxidative stress caused by various environmental stresses. AccATF2 overexpression substantially enhanced the tolerance to oxidative stress of Escherichia coli cells compared with control cells. AccATF2 knockdown significantly increased the production of malondialdehyde (MDA), the transcription of antioxidant genes and the activity of antioxidant enzymes in honeybees, suggesting that AccATF2 knockdown resulted in oxidative damage to honeybees. Moreover, AccATF2 knockdown decreased honeybee resistance to oxidative stress caused by high temperature. Overall, AccATF2 plays an important role in maintaining redox homeostasis and protecting honeybees from oxidative stress caused by various environmental stimuli. Our discoveries add to a growing understanding of how honeybees cope with various adverse environmental conditions to ensure their survival. [Display omitted] • AccATF2 gene was isolated and characterized from Apis cerana cerana. • The expression of AccATF2 was induced by oxidative stress caused by adverse environment. • AccATF2 overexpression enhanced the tolerance of E. coli cells to oxidative stress. • AccATF2 knockdown disrupted the original redox balance and caused oxidative stress. [ABSTRACT FROM AUTHOR]
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- 2022
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22. Identification and antioxidant capacity of 4-hydroxyphenylpyruvate dioxygenase (HPPD), a new favored herbicide target, in Apis cerana cerana.
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Gong, Xiangwei, Zhao, Guangdong, Shan, Wenlu, Guo, Huijuan, Wang, Chen, Liu, Qingxin, Xu, Baohua, Wang, Ying, and Guo, Xingqi
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APIS cerana , *OXIDANT status , *AMINO acid analysis , *HERBICIDES , *ESCHERICHIA coli , *SUPEROXIDE dismutase - Abstract
4-Hydroxyphenylpyruvate dioxygenase (HPPD), a nonheme oxygenase, catalyzes the second step of the tyrosine catabolic pathway, which is shared by almost all aerobic life forms. This demonstrates its importance in aerobic biology. We isolated an HPPD homolog from Apis cerana cerana and named it AccHPPD. AccHPPD has an open reading frame (ORF) length of 900 bp and encodes a 299 amino acid protein that has a predicted molecular weight of 34.67 kDa and an isoelectric point of 6.27. Amino acid analysis showed that AccHPPD contained three conserved metal ion active sites, H-101, H-184 and E-267. Real-time fluorescence quantitative PCR (RT–qPCR) analysis showed that AccHPPD mainly existed in specific tissue sites, mainly high in the legs and in the thorax and epidermis, and in specific developmental stages, mainly adults. Under temperature, pesticide, heavy metal and ultraviolet (UV) radiation treatments, the expression level was downregulated, but under H 2 O 2 treatment, the expression level was upregulated. Exogenous expression of the recombinant AccHPPD plasmid in E. coli enhanced the resistance to HgCl 2 and H 2 O 2. Inhibition of AccHPPD activity was demonstrated by the upregulation of the tyrosine content after feeding with the inhibitor 2-(2-nitro-4-trifluoromethyl benzoyl)-1,3-cyclohexanedione (NTBC). After silencing of AccHPPD , the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) decreased, and the expression levels of AccBax- and AccCaspase8 -related genes were upregulated. The antioxidant genes AccCAT, AccGSTZ1, AccGSTD, AccSOD2, AccTpx3, AccCYP4G11, AccGDTS4, AccGSTO2 and AccMSRA were all upregulated. These results suggest that AccHPPD may serve an integral function in the response of A. cerana cerana to oxidative stress. [Display omitted] • AccHPPD enhances resistance to H 2 O 2. • NTBC inhibition has a time-limited effect. • Silencing of AccHPPD induces apoptotic genes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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23. Identification and characterisation of a novel 1-Cys thioredoxin peroxidase gene (AccTpx5) from Apis cerana cerana.
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Yan, Yan, Zhang, Yuanying, Huaxia, Yifeng, Wang, Xiuling, Yao, Pengbo, Guo, Xingqi, and Xu, Baohua
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THIOREDOXIN , *PEROXIDASE , *APIDAE , *ANTIOXIDANTS , *OXIDATIVE stress , *MOLECULAR weights , *AMINO acids , *GENETIC regulation - Abstract
Abstract: Thioredoxin peroxidases (Tpxs), members of the antioxidant protein family, play critical roles in resisting oxidative stress. In this work, a novel 1-Cys thioredoxin peroxidase gene was isolated from Apis cerana cerana and was named AccTpx5. The open reading frame (ORF) of AccTpx5 is 663bp in length and encodes a 220-amino acid protein with a predicted molecular mass and isoelectric point of 24,921kDa and 5.45, respectively. Promoter sequence analysis of AccTpx5 revealed the presence of putative transcription factor binding sites related to early development and stress responses. Additionally, real-time quantitative PCR (Q-PCR) analysis indicated that AccTpx5 was primarily present in some developmental stages, with the highest expression levels in the first-instar larvae. The expression level of AccTpx5 was up-regulated under various abiotic stresses, including 4°C, 42°C, HgCl2, H2O2, phoxim and acaricide treatments. Conversely, it was down-regulated by UV and pyriproxyfen treatments. Moreover, H2O2 concentration dramatically increased under a variety of stressful conditions. Finally, the purified recombinant AccTpx5 protein protected the supercoiled form of plasmid DNA from damage in the thiol-dependent mixed-function oxidation (MFO) system. These results suggest that AccTpx5 most likely plays an essential role in antioxidant defence. [Copyright &y& Elsevier]
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- 2014
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24. Glutaredoxin 1, glutaredoxin 2, thioredoxin 1, and thioredoxin peroxidase 3 play important roles in antioxidant defense in Apis cerana cerana.
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Yao, Pengbo, Chen, Xiaobo, Yan, Yan, Liu, Feng, Zhang, Yuanying, Guo, Xingqi, and Xu, Baohua
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GLUTAREDOXIN , *THIOREDOXIN , *ANTIOXIDANTS , *APIS cerana , *REACTIVE oxygen species , *SULFHYDRYL group , *DOUBLE-stranded RNA , *NON-coding RNA , *SUPEROXIDE dismutase - Abstract
Abstract: Glutaredoxins (Grxs) and thioredoxins (Trxs) play important roles in maintaining intracellular thiol-redox homeostasis by scavenging reactive oxygen species. However, few Grxs and Trxs have been functionally characterized in Apis cerana cerana. In this study, we identified three genes, AccGrx1, AccGrx2, and AccTrx1, and investigated their connection to antioxidant defense. AccGrx1 and AccGrx2 were mainly detected in dark-eyed pupae, whereas AccTrx1 was highly concentrated in 15-day postemergence adults. The expression levels of AccGrx1 and AccTrx1 were the highest in fat body and epidermis, respectively. However, the expression level of AccGrx2 was the highest in muscle, followed by the epidermis. AccGrx1, AccGrx2, and AccTrx1 were induced by 4, 16, and 42°C; H2O2; and pesticide (acaricide, paraquat, cyhalothrin, and phoxime) treatments and repressed by UV light. AccGrx1 and AccGrx2 were upregulated by HgCl2 treatment, whereas AccTrx1 was downregulated. We investigated the knockdown of AccGrx1, AccGrx2, AccTpx-3, and AccTrx1 in A. cerana cerana and surprisingly found that knockdown of the these four genes enhanced the enzymatic activities of CAT and POD; the metabolite contents of hydrogen peroxide, carbonyls, and ascorbate; and the ratios of GSH/GSSG and NADP+/NADPH. In addition, we also analyzed the transcripts of other antioxidant genes and found that some were upregulated and others were downregulated, revealing that the upregulated genes may be involved in compensating for the knockdown of AccGrx1, AccGrx2, AccTpx-3, and AccTrx1. Taken together, these results suggest that AccGrx1, AccGrx2, AccTpx-3, and AccTrx1 may play critical roles in antioxidant defense. [Copyright &y& Elsevier]
- Published
- 2014
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25. Characterization of a mitochondrial manganese superoxide dismutase gene from Apis cerana cerana and its role in oxidative stress.
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Jia, Haihong, Sun, Rujiang, Shi, Weina, Yan, Yan, Li, Han, Guo, Xingqi, and Xu, Baohua
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SUPEROXIDE dismutase genetics , *MITOCHONDRIAL pathology , *APIS cerana , *OXIDATIVE stress , *MOLECULAR cloning , *GENE expression , *INSECT genetics , *RECOMBINANT proteins , *MANGANESE oxides - Abstract
Highlights: [•] Cloned a mitochondrial manganese superoxide dismutase gene, AccSOD2, from Apis cerana cerana. [•] Examined the expression profiles of AccSOD2 under a variety of oxidative stresses. [•] Evaluated the antioxidant activity of the recombinant AccSOD2 protein under oxidative stresses. [•] Measured the in vivo activities of AccSOD2 in response to oxidative conditions. [ABSTRACT FROM AUTHOR]
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- 2014
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26. Identification of the AccCDK1 gene in Apis cerana cerana and its relationship with the oxidative stress response.
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Peng, Hongyan, Guo, Dezheng, Shan, Wenlu, Liu, Zhenguo, Wang, Hongfang, Ma, Lanting, Xu, Baohua, and Guo, Xingqi
- Subjects
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APIS cerana , *OXIDATIVE stress , *HONEYBEES , *RECOMBINANT proteins , *SUPEROXIDE dismutase , *CLONING - Abstract
The cyclin-dependent kinase (CDK) protein family plays an important role in regulating life functions, such as the cell cycle and metabolism. This study reports the first cloning and functional analysis of A. cerana cerana CDK1 (AccCDK1). The distribution profile of AccCDK1 in different developmental periods and different tissues was determined. The experimental results showed that the distribution of AccCDK1 was tissue-specific. AccCDK1 distribution at the transcriptional and translational levels was affected by stress conditions induced by H 2 O 2 , UV, HgCl 2 , CdCl 2 , extreme temperatures (4 °C, 44 °C) and pesticides (avermectin, lambda-cyhalothrin, haloxyfop- R -methyl, and glyphosate), which resulted in changes in the expression levels. These results suggest that AccCDK1 may have an important part to play in honey bee resistance to stress. The expression of a recombinant AccCDK1 protein in vitro enhanced the antistress capacities of E. coli and yeast, which suggests that AccCDK1 is related to the stress response. When AccCDK1 was silenced, the expression of some antioxidant genes was downregulated, and the enzymatic potencies of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were reduced, which suggests that AccCDK1 takes part in the body's resistance to oxidative stress upon external stimulation by influencing relevant antioxidants. Notably, the survival rate of A. cerana cerana under high-temperature-induced stress decreased after AccCDK1 silencing, which verifies our results. In conclusion, we found that AccCDK1 played an indispensable function in resisting oxidative stress and maintaining normal cellular functions. [Display omitted] • Oxidative stress can affect the expression of cyclin-dependent kinase1. • AccCDK1 silencing affects cellular antioxidant genes and enzymes. • AccCDK1 promotes the survival of eukaryotic and prokaryotic cells under stress. • AccCDK1 promotes bees to resist high temperature stress. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
27. Molecular cloning, expression and oxidative stress response of a mitochondrial thioredoxin peroxidase gene (AccTpx-3) from Apis cerana cerana
- Author
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Yao, Pengbo, Lu, Wenjing, Meng, Fei, Wang, Xiuling, Xu, Baohua, and Guo, Xingqi
- Subjects
- *
MOLECULAR cloning , *GENE expression , *INSECT genetics , *OXIDATIVE stress , *THIOREDOXIN , *INSECT physiology , *PEROXIDASE , *APIS cerana , *INSECT enzymes , *HOMEOSTASIS - Abstract
Abstract: Thioredoxin peroxidase (Tpxs) plays an important role in maintaining redox homeostasis and in protecting organisms from the accumulation of toxic reactive oxygen species (ROS). Here, we isolated a mitochondrial thioredoxin peroxidase gene from Apis cerana cerana, AccTpx-3. The open reading frame (ORF) of AccTpx-3 is 729bp in length and encodes a predicted protein of 242 amino acids, 27.084kDa and an isoelectric point of 8.70. Furthermore, the 980bp 5′ flanking region was cloned, and the transcription factor binding sites were predicted. A quantitative RT-PCR (Q-PCR) analysis indicated that AccTpx-3 was expressed higher in muscle than other tissues, with its highest expression occurring on the fourth day of the larval stage, followed by the fifteenth day of the adult stage. Moreover, the expression of the AccTpx-3 transcript was upregulated by such abiotic stresses as 4°C, 42°C, H2O2, cyhalothrin, acaricide and phoxime treatments. In contrast, AccTpx-3 transcription was downregulated by other abiotic stresses, including 16°C, 25°C, ultraviolet light and HgCl2. Recombinant AccTpx-3 protein acted as a potent antioxidant that resisted paraquat-induced oxidative stress and protected DNA from oxidative damage. Taken together, these results suggest that the AccTpx-3 protein is an antioxidant enzyme that may protect organisms from oxidative stress. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
28. The identification and oxidative stress response of a zeta class glutathione S-transferase (GSTZ1) gene from Apis cerana cerana
- Author
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Yan, Huiru, Meng, Fei, Jia, Haihong, Guo, Xingqi, and Xu, Baohua
- Subjects
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GLUTATHIONE transferase , *OXIDATIVE stress , *APIS cerana , *ANTISENSE DNA , *MESSENGER RNA , *DINITROBENZENES - Abstract
Abstract: Glutathione-S-transferases (GSTs) play an important role in protecting organisms against the toxicity of reactive oxygen species (ROS). However, no information is available for GSTs in the Chinese honey bee (Apis cerana cerana). In this study, we isolated and characterized a zeta class GST gene (AccGSTZ1) from the Chinese honey bee. This gene is present in a single copy and harbors five exons. The deduced amino acid sequence of AccGSTZ1 shared high sequence identity with homologous proteins and contained the highly conserved features of this gene family. The temporal and spatial expression profiles of AccGSTZ1 showed that AccGSTZ1 was highly expressed in fourth instar larvae during development, and the mRNA level of AccGSTZ1 was higher in the epidermis than that in other tissues. The expression pattern under oxidative stress revealed that AccGSTZ1 transcription was significantly upregulated by external factors, such as temperature challenges and H2O2 treatment. The characterization of the purified protein revealed that AccGSTZ1 had low glutathione-conjugating activity, but the recombinant AccGSTZ1 protein displayed high antioxidant activity under oxidative stress. These data suggest that AccGSTZ1 is an oxidative stress-inducible antioxidant enzyme that plays an important role in the protection against oxidative stress and may be of critical importance for the survival of the honey bees. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
29. Identification and characterization of an Apis cerana cerana nucleoside diphosphate kinase (AccNDPK) associated with oxidative stress.
- Author
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Zhao, Guangdong, Zhang, Xuemei, Guo, Dezheng, Wang, Hongfang, Guo, Hengjun, Tian, Ming, Sun, Qinghua, Li, Han, Xu, Baohua, and Guo, Xingqi
- Subjects
- *
APIS cerana , *OXIDATIVE stress , *CYHALOTHRIN , *HONEYBEES , *SUPEROXIDE dismutase - Abstract
Nucleoside diphosphate kinases (NDPKs) are widespread nucleotide-metabolizing enzymes that are involved in a variety of biological processes, including responses to oxidative stress. Although studies have been conducted on NDPKs in mammals and some plants, there is scant research on insect NDPKs, especially in honey bees. In the present study, we isolated AccNDPK from Apis cerana cerana. Sequence analysis showed that AccNDPK has high homology with many NDPKs and contains a highly conserved NDPK active site motif. Based on phylogenetic analysis, AccNDPK has a relatively recent evolutionary relationship with NDPKs in other hymenopteran insects. AccNDPK was found to be highly expressed in newly emerged honey bees and muscle tissues, and RT-qPCR analysis and bacteriostatic assays showed that the expression level of AccNDPK is affected by abnormal temperature, UV light, H 2 O 2 , heavy metals, and various pesticides. After AccNDPK knockdown, antioxidant-related genes, including AccCAT , AccCYP4G11 , AccGSTS4 , AccTpx1 and AccMsrA , were upregulated, whereas AccGSTD , AccGST1 , AccHSP22.6 and AccTrx1 were downregulated. Furthermore, catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities were significantly increased, and the tolerance of bees to oxidative stress caused by cyhalothrin was reduced by silencing of AccNDPK. Given these findings, we speculate that AccNDPK plays an important role in the oxidative stress response of A. cerana cerana. [Display omitted] • AccNDPK was induced by various environmental stresses. • Expression of recombinant AccNDPK enhances resistance of E. coli to oxidative stress. • Knockdown of AccNDPK affected several antioxidant-related genes and the activities of antioxidant-related enzymes. • The tolerance to cyhalothrin of A. cerana cerana was reduced after AccNDPK knockdown. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
30. AccPDIA6 from Apis cerana cerana plays important roles in antioxidation.
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Meng, Jie, Wang, Lijun, Wang, Chen, Zhao, Guangdong, Wang, Hongfang, Xu, Baohua, and Guo, Xingqi
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APIS cerana , *PROTEIN disulfide isomerase , *SURVIVAL rate , *MOLECULAR chaperones , *WESTERN immunoblotting , *ISOMERASES , *GENE silencing , *IMIDACLOPRID - Abstract
PDIA6 is a member of the protein disulfide isomerase (PDI) family, shows disulfide isomerase activity and oxidoreductase activity, and can act as a molecular chaperone. Its biological functions include modulating apoptosis, regulating the proliferation and invasion of cancer cells, supporting thrombosis and modulating insulin secretion. However, the roles of PDIA6 in Apis cerana cerana are poorly understood. Herein, we obtained the PDIA6 gene from A. cerana cerana (AccPDIA6). We investigated the expression patterns of AccPDIA6 in response to oxidative stress induced by H 2 O 2 , UV, HgCl 2 , extreme temperatures (4 °C, 42 °C) and pesticides (thiamethoxam and hexythiazox) and found that AccPDIA6 was upregulated by these treatments. Western blot analysis indicated that AccPDIA6 was also upregulated by oxidative stress at the protein level. In addition, a survival test demonstrated that the survival rate of E. coli cells expressing AccPDIA6 increased under oxidative stress, suggesting a possible antioxidant function of AccPDIA6. In addition, we tested the transcripts of other antioxidant genes and found that some of them were downregulated in AccPDIA6 knockdown samples. It was also discovered that the antioxidant enzymatic activity of superoxide dismutase (SOD) decreased in AccPDIA6- silenced bees. Moreover, the survival rate of AccPDIA6 knockdown bees decreased under oxidative stress, implying that AccPDIA6 may function in the oxidative stress response by enhancing the viability of honeybees. Taken together, these results indicated that AccPDIA6 may play an essential role in counteracting oxidative stress. [Display omitted] • AccPDIA6 may participate in the oxidative stress response. • Overexpression of AccPDIA6 improved the antioxidant capacity of E. coli cells. • Knockdown of AccPDIA6 affected the oxidative system. • AccPDIA6 gene silencing reduced the survival of A. cerana cerana. • Cysteines of CGHC motifs are crucial for the anti-stress activities of AccPDIA6. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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