1. miRNA-381-3p Functions as a Tumor Suppressor to Inhibit Gastric Cancer by Targeting Fibroblast Growth Factor Receptor-2.
- Author
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Gao, Xiang, Liu, Huiqi, Wu, Qiong, Wang, Rong, Huang, Mingyu, Ma, Qiang, and Liu, Yongnian
- Subjects
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STOMACH tumors , *FIBROBLAST growth factors , *FLOW cytometry , *CANCER invasiveness , *MICRORNA , *CELL receptors , *APOPTOSIS , *METASTASIS , *GENE expression , *CELL motility , *TUMOR suppressor genes , *TISSUES , *RESEARCH funding , *CELL proliferation , *CELL lines , *POLYMERASE chain reaction - Abstract
Objectives: MicroRNAs possess essential effects on gastric cancer (GC), whereas the underlying mechanisms have not been fully uncovered. The present work focused on investigating the role of miR-381-3p in GC cellular processes and the possible mechanisms. Materials and Methods: miR-381-3p levels within GC tissues and cells were measured through quantitative real-time polymerase chain reaction (qRT-PCR). This study measured cell proliferation, apoptosis, and metastasis through EdU, colony formation, flow cytometry, and Transwell assays separately. TargetScan was adopted to predict the miR-381-3p targets, whereas luciferase reporter assay was adopted for confirmation. Results: miR-381-3p levels were decreased, whereas fibroblast growth factor receptor-2 (FGFR2) expression was increased in GC. miR-381-3p upregulation inhibited proliferation, migration, and invasion and it promoted the apoptosis of GC cells. Further, FGFR2 overexpression partly reversed the miR-381-3p-mediated impacts on GC cellular processes. Conclusions: This study provides an experimental basis, suggesting the potential of using miR-381-3p as the novel marker for GC. Clinical Trial Registration number: 2020-05. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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