1. Downregulation of miR-21 modulates Ras expression to promote apoptosis and suppress invasion of Laryngeal squamous cell carcinoma
- Author
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Ren, Jingyuan, Zhu, Dan, Liu, Ming, Sun, Yanan, and Tian, Linli
- Subjects
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SQUAMOUS cell carcinoma , *CANCER treatment , *LARYNGEAL muscles , *APOPTOSIS , *CANCER cells , *GENE expression , *CANCER , *RNA physiology , *CANCER invasiveness , *CELL culture , *FLOW cytometry , *IMMUNOHISTOCHEMISTRY , *LARYNGEAL cancer , *POLYMERASE chain reaction , *PROBABILITY theory , *RESEARCH funding , *STATISTICAL hypothesis testing , *T-test (Statistics) , *WESTERN immunoblotting , *REVERSE transcriptase polymerase chain reaction - Abstract
MiRNAs are small, noncoding RNA molecules that emerge as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety of cancers and has been causally related to cellular proliferation and apoptosis. In this study, we found that miR-21 is overexpressed in Laryngeal squamous cell carcinoma (LSCC) and correlated with advanced stage. Inhibition of miR-21 by antisense oligonucleotides (ASO) led to decreased protein level of Ras and profound suppression of cell proliferation and invasion. Hep-2 cells exposed to miR-21 ASO exhibited cell cycle arrest at G1 phase and increased apoptosis. Furthermore, growth of LSCC xenograft tumours was significantly suppressed by repeated injection of ASO-miR-21 lentivirus and the Ras protein expression in LSCC xenograft tumours was also downregulate by ASO-miR-21. Taken together, our data suggest that miR-21 may play an oncogenic role in the cellular processes of LSCC and represent a novel target for effective therapies. [Copyright &y& Elsevier]
- Published
- 2010
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