1. Self-assembled dihydroartemisinin nanoparticles as a platform for cervical cancer chemotherapy
- Author
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Bo Yang, Jia Luo, BiQiong Wang, Yue Chen, Yun Lu, ShaoZhi Fu, Qian Wen, Kang Xiong, and ZhouXue Wu
- Subjects
cervical cancer ,medicine.medical_treatment ,Uterine Cervical Neoplasms ,Pharmaceutical Science ,Nanoparticle ,Apoptosis ,Biocompatible Materials ,02 engineering and technology ,Pharmacology ,030226 pharmacology & pharmacy ,Polyethylene Glycols ,Mice ,dihydroartemisinin ,chemistry.chemical_compound ,Drug Delivery Systems ,0302 clinical medicine ,media_common ,Cervical cancer ,Drug Carriers ,Cell Cycle ,General Medicine ,021001 nanoscience & nanotechnology ,Artemisinins ,Treatment Outcome ,Original Article ,Female ,0210 nano-technology ,Research Article ,Drug ,Polyesters ,media_common.quotation_subject ,Mice, Nude ,Dihydroartemisinin ,RM1-950 ,Self assembled ,Antimalarials ,03 medical and health sciences ,parasitic diseases ,medicine ,Animals ,Humans ,Chemotherapy ,medicine.disease ,Bioavailability ,chemistry ,nanoparticles ,Therapeutics. Pharmacology ,mpeg-pcl ,Ethylene glycol ,HeLa Cells - Abstract
Dihydroartemisinin (DHA) is a potent anti-cancer drug that has limited clinical applications due to poor water solubility and low bioavailability. We designed a biodegradable poly(ethylene glycol) methyl ether-poly(ε-caprolactone) (MPEG-PCL) micelle carrier for DHA using the self-assembly method. The DHA/MPEG-PCL nanoparticles were spherical with an average particle size of 30.28 ± 0.27 nm, and released the drug in a sustained manner in aqueous solution. The drug-loaded nanoparticles showed dose-dependent toxicity in HeLa cells by inducing cycle arrest and apoptosis. Furthermore, compared to free DHA, the DHA/MPEG-PCL nanoparticles showed higher therapeutic efficacy and lower toxicity in vivo, and significantly inhibited tumor growth and prolonged the survival of tumor-bearing nude mice. In addition, the tumor tissues of the DHA/MPEG-PCL-treated mice showed a marked decline in the in situ expression of proliferation and angiogenesis markers. Taken together, the self-assembled DHA/MPEG-PCL nanoparticles are a highly promising delivery system for targeted cancer treatment.
- Published
- 2020