1. A Study on Curcumol Influencing Proliferation and Apoptosis of Hepatocellular Carcinoma Cells through DJ-1/PTEN/PI3K/AKT Pathway.
- Author
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Zhang, Rongzhen, Zhong, Lu, Sun, Kewei, Liu, Jiao, Wang, Qianna, Mao, Dewen, Fang, Gang, and Long, Fuli
- Subjects
IN vitro studies ,FLOW cytometry ,REVERSE transcriptase polymerase chain reaction ,HERBAL medicine ,WESTERN immunoblotting ,APOPTOSIS ,CELLULAR signal transduction ,GENE expression ,CELL proliferation ,CELL lines ,CHINESE medicine ,HEPATOCELLULAR carcinoma ,DRUG administration ,DRUG dosage ,THERAPEUTICS - Abstract
Objective. To study the mechanism of curcumol affecting the proliferation and apoptosis of liver cancer cells through the DJ-1/PTEN/PI3K/AKT pathway. Method. HepG2 cells were cultured in vitro, treated with curcumol at concentrations of 10, 30, and 100 μg/mL, and DMSO was used as a control. The levels of cell proliferation and apoptosis were measured by CCK-8 and flow cytometry, respectively. RT-PCR and western blot were used to detect PTEN, p-AKT, DJ-1, and PI3K gene and protein expression changes. Result. (1) Compared with the DMSO blank control group, the proliferation level of liver cancer cells in the 10 μg/mL curcumol group decreased, and the proportion of apoptosis increased (p <0.05). (2) Compared with the blank control group and the 10 and 30 μg/mL concentration groups, the proliferation level of liver cancer cells in the 100 μg/mL curcumol group was significantly reduced, and the proportion of cell apoptosis was significantly increased (p < 0.05). (3) Curcumol can significantly increase the expression of PTEN gene and protein in liver cancer cells and reduce the expression of DJ-1 and PI3K genes and protein in liver cancer cells (p < 0.05). Conclusion. Curcumol can regulate DJ-1, PTEN, PI3K, and AKT signal transduction pathways, inhibit cell proliferation, and cause a significant increase in the proportion of cell apoptosis, and the pharmacodynamic effect of curcumol is dependent on the time and dose of action. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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