1. Regulatory Effects of Black Rice Extract on Helicobacter pylori Infection-Induced Apoptosis.
- Author
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Kim SH, Lee MH, Park M, Woo HJ, Kim YS, Tharmalingam N, Seo WD, and Kim JB
- Subjects
- Adenosine Triphosphatases genetics, Adenosine Triphosphatases metabolism, Anthocyanins administration & dosage, Anthocyanins analysis, Anthocyanins pharmacology, Antigens, Bacterial genetics, Bacterial Proteins genetics, Bacterial Proteins metabolism, Cell Line, Tumor, Functional Food, Gene Expression Regulation, Bacterial drug effects, Glucosides administration & dosage, Glucosides analysis, Glucosides pharmacology, Helicobacter Infections pathology, Helicobacter pylori genetics, Helicobacter pylori pathogenicity, Humans, Plant Extracts chemistry, SEC Translocation Channels genetics, SEC Translocation Channels metabolism, SecA Proteins, Stomach Neoplasms pathology, Stomach Neoplasms virology, Virulence Factors genetics, Virulence Factors metabolism, Apoptosis drug effects, Helicobacter Infections diet therapy, Oryza chemistry, Plant Extracts pharmacology
- Abstract
Scope: Black rice extract (BRE) contains cyanidin 3-O-glucoside (C3G), an anthocyanin, as the major component. In this study, we found that BRE inhibits the mRNA and protein expression of genes encoding cytotoxin-associated protein A (cagA) and vacuolating protein A (vacA) in Helicobacter pylori 60190 strain., Methods and Results: We performed RT-PCR and western blotting to show that BRE inhibits the mRNA and protein expression of SecA. Because SecA is involved in VacA export in bacteria, our result suggests a positive correlation between BRE-induced inhibition of secA expression and VacA secretion. Further, we perform MTT assay and flow cytometry to show that BRE decreases the apoptosis of H. pylori-infected KATO III cells. Finally, we perform western blotting to show that the cell-protective effect of BRE is associated with decreased levels of active proapoptotic proteins caspases and PARP and increased levels of antiapoptotic proteins survivin and XIAP in H. pylori-infected cells., Conclusion: Thus, our results indicate that BRE acts as a potent inhibitor of the biogenesis of H. pylori virulence proteins and decreases the apoptosis of H. pylori-infected cells. Moreover, our results suggest that BRE can be used to exert beneficial effects in patients with gastroduodenal diseases caused by H. pylori., (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) more...
- Published
- 2018
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