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3 results on '"Movassagh Pour A"'

1. Vitamin D-Loaded Nanostructured Lipid Carrier (NLC): A New Strategy for Enhancing Efficacy of Doxorubicin in Breast Cancer Treatment

Author

Jamal Mohammadian, Maryam Mohammadi, Mehdi Sabzichi, Farhad Jahanfar, Hamed Hamishehkar, Ali Akbar Movassagh Pour, and Alireza Ostadrahimi

Subjects
Cancer Research, Stereochemistry, Survivin, medicine.medical_treatment, Medicine (miscellaneous), Antineoplastic Agents, Apoptosis, Breast Neoplasms, 02 engineering and technology, Inhibitor of Apoptosis Proteins, Inhibitory Concentration 50, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Text mining, medicine, Vitamin D and neurology, Humans, Inhibitory concentration 50, Doxorubicin, Particle Size, Vitamin D, Cell Proliferation, bcl-2-Associated X Protein, Regulation of gene expression, Drug Carriers, Chemotherapy, Nutrition and Dietetics, business.industry, Chemistry, Cell Cycle Checkpoints, 021001 nanoscience & nanotechnology, medicine.disease, Nanostructures, Gene Expression Regulation, Proto-Oncogene Proteins c-bcl-2, Oncology, 030220 oncology & carcinogenesis, MCF-7 Cells, Cancer research, Female, 0210 nano-technology, business, medicine.drug
Abstract

The dangerous dose-dependent side effects of anticancer agents triggered the finding of new approaches for elevated chemotherapy efficacy. This study investigated the potential application of nanostructured lipid careers (NLCs) in increasing vitamin D

Published
2017
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2. Developing and characterization of single chain variable fragment (scFv) antibody against frizzled 7 (Fzd7) receptor

Author

Mehdi Yousefi, Hamid Nickho, Morteza Motallebnezhad, Aliakbar Movassagh Pour, Leili Aghebati-Maleki, Jafar Majidi Zolbanin, and Vahid Younesi

Subjects
0301 basic medicine, Frizzled, Phage display, Apoptosis, Enzyme-Linked Immunosorbent Assay, Triple Negative Breast Neoplasms, Bioengineering, Biology, Applied Microbiology and Biotechnology, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Humans, Single-chain variable fragment, Receptor, Immunoassay, Wnt signaling pathway, General Medicine, Molecular biology, Frizzled Receptors, Treatment Outcome, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer cell, biology.protein, Cancer research, Drug Screening Assays, Antitumor, Signal transduction, Antibody, Cell Surface Display Techniques, Research Paper, Single-Chain Antibodies, Biotechnology
Abstract

ABSTACT Wnt/β-catenin signaling pathway through Frizzled receptors has been shown to play a key role in both normal development and tumorigenesis. Overexpression of Wnt pathway genes, such as Fzd7 in several malignancies is well-documented. Therefore, targeting of Fzd7 and its ligand inhibits cancer cells proliferation metastasis. In the present study we isolated single chain variable fragments (scFvs) against Fzd7 receptor using phage display method. Semi-synthetic human naive antibody libraries (Tomlinson I + J) was employed in panning procedure to isolate specific scFv against specific peptide from extracellular domain of Fzd7 receptor. The reactivity and growth inhibition effects of the selected antibodies was evaluated using enzyme-linked immunosorbent assay (ELISA), MTT and annexin V assays, respectively. Seven scFvs reactive to Fzd7 were selected following 4 rounds of panning. The results showed that the selected scFvs inhibits cell growth through apoptosis cell death in a triple negative breast cancer cells, MDA-MB-231. Given that Fzd7 and Wnt pathway plays a critical role in tumor progression, selected blocking scFvs represent significant potential for immunotherapy of breast cancer cells.

Published
2017
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3. Vitamin D-Loaded Nanostructured Lipid Carrier (NLC): A New Strategy for Enhancing Efficacy of Doxorubicin in Breast Cancer Treatment.

Author

Sabzichi, Mehdi, Mohammadian, Jamal, Mohammadi, Maryam, Jahanfar, Farhad, Movassagh Pour, Ali Akbar, Hamishehkar, Hamed, and Ostad-Rahimi, Alireza

Subjects
BREAST tumor prevention, CELL proliferation, APOPTOSIS, BIOLOGICAL assay, BIOLOGICAL models, COMBINATION drug therapy, DOXORUBICIN, DRUG synergism, DOSE-effect relationship in pharmacology, FLOW cytometry, GENE expression, MICROSCOPY, NANOSTRUCTURES, POLYMERASE chain reaction, PROBABILITY theory, SPECTRUM analysis, STAINS & staining (Microscopy), STATISTICAL significance, CHOLECALCIFEROL, DESCRIPTIVE statistics, IN vitro studies, PHARMACODYNAMICS
Abstract

The dangerous dose-dependent side effects of anticancer agents triggered the finding of new approaches for elevated chemotherapy efficacy. This study investigated the potential application of nanostructured lipid careers (NLCs) in increasing vitamin D3 (VitD) effectiveness in breast cancer cell (MCF-7) in concurrent administration with doxorubicin (Dox). VitD-loaded NLCs were characterized by particle size, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscope. Cytotoxicity and molecular effects of formulation were evaluated by MTT, DAPI staining, flow cytometry, and real-time quantitative PCR assays. The formulation revealed mean particle size of 87±5 nm with a polydispersity index of 0.24 confirmed by SEM images. The IC50 values for VitD and Dox were 1.3 ± 0.04 and 0.65 ± 0.05 µM, respectively. VitD-loaded NLCs decreased the percentage of cell proliferation from 49 ± 7.2% to 37 ± 5.1% (P < 0.05). Cotreatment of the cells with VitD-loaded NLCs and Dox caused over a twofold increase in the percentage of apoptosis (P < 0.05). Gene expression profile demonstrated a significant decrease in antiapoptotic factor survivin along with increase in proapoptotic factor Bax mRNA levels. Overall, our results introduced the NLC technology as a novel strategy to elevate the efficacy of chemotherapeutics in breast cancer. [ABSTRACT FROM AUTHOR]

Published
2017
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