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3 results on '"Muhan Huang"'

1. SARS-CoV-2 Membrane Glycoprotein M Triggers Apoptosis With the Assistance of Nucleocapsid Protein N in Cells

Author

Yujie Ren, An Wang, Yuan Fang, Ting Shu, Di Wu, Chong Wang, Muhan Huang, Juan Min, Liang Jin, Wei Zhou, Yang Qiu, and Xi Zhou

Subjects
SARS-CoV-2, nucleocapsid protein, apoptosis, PDK1-Akt signaling, membrane glycoprotein, Microbiology, QR1-502
Abstract

The pandemic of COVID-19 by SARS-CoV-2 has become a global disaster. However, we still don’t know how specific SARS-CoV-2-encoded proteins contribute to viral pathogenicity. We found that SARS-CoV-2-encoded membrane glycoprotein M could induce caspase-dependent apoptosis via interacting with PDK1 and inhibiting the activation of PDK1-PKB/Akt signaling. Our investigation further revealed that SARS-CoV-2-encoded nucleocapsid protein N could specifically enhance the M-induced apoptosis via interacting with both M and PDK1, therefore strengthening M-mediated attenuation of PDK1-PKB/Akt interaction. Furthermore, when the M-N interaction was disrupted via certain rationally designed peptides, the PDK1-PKB/Akt signaling was restored, and the boosting activity of N on the M-triggered apoptosis was abolished. Overall, our findings uncovered a novel mechanism by which SARS-CoV-2-encoded M triggers apoptosis with the assistance of N, which expands our understanding of the two key proteins of SARS-CoV-2 and sheds light on the pathogenicity of this life-threatening virus.

Published
2021
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2. Dual inhibition of innate immunity and apoptosis by human cytomegalovirus protein UL37x1 enables efficient virus replication

Author

Yujie Ren, An Wang, Di Wu, Chong Wang, Muhan Huang, Xiaobei Xiong, Liang Jin, Wei Zhou, Yang Qiu, and Xi Zhou

Subjects
Microbiology (medical), Viral Structural Proteins, Immunology, Cytomegalovirus, Apoptosis, Cell Biology, Virus Replication, Applied Microbiology and Biotechnology, Microbiology, Immunity, Innate, Immediate-Early Proteins, Mice, Genetics, Animals, Humans, Immune Evasion
Abstract

Immune evasion and inhibition of apoptosis are required for successful virus infection. However, inhibition of apoptosis can increase antiviral immune responses, which can then clear viral infections. Here we show that human cytomegalovirus (HCMV)-encoded UL37 exon-1 protein (UL37x1) not only inhibits apoptosis but also suppresses the cGAS-STING immune pathway. Using co-immunoprecipitation assays, we found that UL37x1 binds to TBK1 to abrogate the TBK1-STING-IRF3 interaction. Although the anti-apoptosis function of UL37x1 increases immune signalling, the immunosuppressive role of UL37x1 counteracts this undesirable side-effect. Furthermore, we used mutational analyses to show that the loss of either immunosuppressive or anti-apoptotic function of UL37x1 significantly reduced HCMV replication in human primary foreskin fibroblasts and humanized mice by over twofold. Finally, loss of both functions resulted in over fourfold reduction of HCMV replication in the same cell type and mouse model, showing that both UL37x1 functions are crucial for HCMV infection. We conclude that this sophisticated mechanism enables HCMV to control innate immunity and apoptosis to ensure efficient infection.

Published
2021

3. The ORF3a protein of SARS-CoV-2 induces apoptosis in cells

Author

Wei Zhou, Yang Qiu, Jingfang Mu, Xue Yi Zhang, Yujie Ren, Di Wu, Chong Wang, Muhan Huang, Yang Han, Ting Shu, and Xi Zhou

Subjects
2019-20 coronavirus outbreak, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Immunology, Pneumonia, Viral, Caspase 3, Apoptosis, Biology, Transfection, Viroporin Proteins, Betacoronavirus, Correspondence, Cell death and immune response, Chlorocebus aethiops, Immunology and Allergy, Animals, Humans, Viral Regulatory and Accessory Proteins, Pandemics, Vero Cells, Viral Structural Proteins, SARS-CoV-2, Immune cell death, HEK 293 cells, COVID-19, Hep G2 Cells, biology.organism_classification, Cell biology, Infectious Diseases, HEK293 Cells, Vero cell, Coronavirus Infections
Published
2020

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