1. Interferon (IFN)-beta induces apoptotic cell death in DHL-4 diffuse large B cell lymphoma cells through tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
- Author
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Oehadian A, Koide N, Mu MM, Hassan F, Islam S, Yoshida T, and Yokochi T
- Subjects
- Apoptosis Regulatory Proteins, Cell Proliferation, DNA Damage, Gene Expression Profiling, Humans, Interferon-alpha pharmacology, Interferon-gamma pharmacology, TNF-Related Apoptosis-Inducing Ligand, Tumor Cells, Cultured, Antineoplastic Agents pharmacology, Apoptosis, Interferon-beta pharmacology, Lymphoma, B-Cell pathology, Lymphoma, Large B-Cell, Diffuse pathology, Membrane Glycoproteins biosynthesis, Membrane Glycoproteins physiology, Tumor Necrosis Factor-alpha biosynthesis, Tumor Necrosis Factor-alpha physiology
- Abstract
The effect of interferon (IFN)-alpha, beta and gamma on the growth of DHL-4 diffuse large B cell lymphoma cells was studied. IFN-beta significantly inhibited the cell growth, and the effect was stronger than that of IFN-alpha. IFN-gamma did not inhibit the cell growth because of lack of IFN-gamma receptors. IFN-beta caused apoptotic cell death which was accompanied by DNA fragmentation, caspase 3 activation and annexin V binding. IFN-beta lead to the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA. Anti-TRAIL antibody significantly prevented IFN-beta-induced apoptosis. It was suggested that IFN-beta might cause apoptosis in DHL-4 cells through TRAIL.
- Published
- 2005
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