1. lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer.
- Author
-
Song L, Wang L, Pan X, and Yang C
- Subjects
- Apoptosis genetics, Blotting, Western, Cell Line, Tumor, Cell Proliferation genetics, Female, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs genetics, RNA, Long Noncoding genetics, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation, Uterine Cervical Neoplasms metabolism, rho-Associated Kinases genetics, Apoptosis physiology, Cell Proliferation physiology, MicroRNAs metabolism, RNA, Long Noncoding metabolism, Uterine Cervical Neoplasms pathology, rho-Associated Kinases metabolism
- Abstract
Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of cervical cancer progression via interacting with miR-143-3p to regulate ROCK1 expression. Our study revealed novel mechanisms about how lncRNA OIP5-AS1 executed its function in cervical cancer and thus provided potential therapeutic targets for the disease.
- Published
- 2020
- Full Text
- View/download PDF