1. [Expression of X-linked inhibitor of apoptosis protein in Tca8113 cell and its relationship to chemoresistance].
- Author
-
Wang JF, Zhang ZM, Wang CK, Nie DB, and Gao WX
- Subjects
- Bleomycin analogs & derivatives, Carcinoma, Squamous Cell, Drug Resistance, Neoplasm, Humans, Apoptosis, Cell Line, Tumor, X-Linked Inhibitor of Apoptosis Protein
- Abstract
Objective: To explore the expression of X-linked inhibitor of apoptosis protein (XIAP) in Tca8113 cell, and to investigate its relationship to the chemoresistance. METHODS; The Tca8113 cell line was cultured by IMDM and the concentration of Pingyangmycin (PYM) added to Tca8113 cell line was increased gradually and continually, which was to induce the PYM-resistance in Tca8113 cell line. The sensitivity of Tca8113 cell to PYM and expression of XIAP were measured with methyl thiazolyl tetrazolium (MTT) chromatometry and reverse transcription-polymerase chain raction (RT-PCR). The XIAP level in the cells and its chemoresistance to PYM were analyzed by linear regression., Results: The IC50 of Tca8113-1-10 group and Tca8113-10-10 group were(12.758 +/- 0.030), (18.986 +/- 0.150) microg x mL(-1) respectively. The IC50 of Tca8113-1-20 group and Tca8113-10-20 group increased to (26.302 +/- 0.072), (35.294 +/- 0.115) microg x mL(-1) respectively. There was a relation between XIAP and the drug-resistance in Tca8113 cell., Conclusion: XIAP may play an important role in the chemoresistance which might serve as a new therapeutic target for oral squamous cell carcinoma.
- Published
- 2010