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Jianghong Hu,1,* Guohua Ni,2,*Ling Mao,2 Xianglong Xue,1 Jijie Zhang,2 Weixia Wu,2 Shaoru Zhang,3 Hong Zhao,3 Lifang Ding,2 Lihui Wang3 1Department of Gastroenterology, Danyang People’s Hospital of Jiangsu Province and Danyang Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, People’s Republic of China; 2Department of Oncology, Danyang People’s Hospital of Jiangsu Province and Danyang Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, People’s Republic of China; 3Central Laboratory, Danyang People’s Hospital of Jiangsu Province and Danyang Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong Zhao; Lihui WangDanyang People’s Hospital of Jiangsu Province, 2 Xinmin West Road, Danyang 212300, People’s Republic of ChinaTel +86 511 8658 0096Fax +86 511 8655 3198Email zhjszj@163.com; njuwanglihui@163.comBackground: Numerous studies have shown that long noncoding RNA (lncRNA) is involved in gastric cancer (GC). A relevant microarray containing gastric cancer-related lncRNAs was downloaded from The Cancer Genome Atlas database.Methods: qRT-PCR was used to analyze LINC00565 and AKT3 expression in tumor tissues and cell lines. Proliferative, colony formation and apoptotic abilities of GC cells after transfection of sh-LINC00565 were determined by CCK-8, colony formation assay and flow cytometry, respectively. RIP was enrolled to detect the interaction between LINC00565, AKT3 and miR-665. Dual luciferase assay was used to confirm the relation between miR-665 and LINC00565 and AKT3.Results: Expression level of LINC00565 in GC tissue was highly expressed in GC, which was negatively correlated to prognosis of GC patients. The results showed that knockdown of LINC00565 decreased proliferative and colony formation abilities, and induced apoptosis of GC cells. Pearson analysis showed that LINC00565 was positively correlated with AKT3. Besides, AKT3 was significantly up-regulated in GC. In addition, knockdown of LINC00565 down-regulated AKT3. In order to explore the mechanism, we found that miR-665 could bind to LINC00565 by bioinformatics. Dual-luciferase reporter gene assay and RIP assay both verified the binding relationship between miR-665 and AKT3. Finally, rescue experiments were carried out to explore whether AKT3 could reverse the anti-cancer effect of low-level LINC00565 on GC development.Conclusion: In summary, the expression of LINC00565 is upregulated in GC. LINC00565 can be used as the sponge of miR-665 to up-regulate the expression of AKT3, thus promoting the progression of GC.Keywords: CeRNA, GC, LINC00565, MicroRNA-665, AKT3, proliferation, apoptosis