1. Regulation of Lnc-NTF3-5 on islet β-cell dysfunction in high glucose environment and related mechanisms.
- Author
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Zhang XL, Zhang Y, Zhu HQ, Zhang CY, Jiao JS, and Xing XY
- Subjects
- Animals, Cell Line, Tumor, Cell Proliferation genetics, Culture Media metabolism, Diabetes Mellitus pathology, Humans, Insulin metabolism, Insulin Secretion genetics, Insulin-Secreting Cells metabolism, Interleukin-1 metabolism, Interleukin-10 metabolism, Mice, RNA, Long Noncoding genetics, RNA, Small Interfering metabolism, Apoptosis genetics, Diabetes Mellitus genetics, Glucose metabolism, Insulin-Secreting Cells pathology, RNA, Long Noncoding metabolism
- Abstract
Objective: The pathogenesis of diabetes is closely related to islet β-cell dysfunction. Lnc-NTF3-5 participates in the occurrence and development of various diseases. However, Lnc-NTF3-54's effect on islet β-cell dysfunction in high glucose environment remains unclear., Materials and Methods: The islet β cell MIN6 cells were cultured in vitro and randomly divided into control group, high glucose group, NTF3-5 siRNA group, and NTF3-5 group, which was respectively transfected with Lnc-NTF3-5 siRNA and Lnc-NTF3-5 plasmid under high glucose condition. Lnc-NTF3-5 expression was measured by real time PCR and cell proliferation was assessed by MTT assay. In addition, Caspase 3 activity, SOD activity, and ROS content were also detected along with the secretion of IL-10 and IL-1 by enzyme-linked immunosorbent assay (ELISA)., Results: Compared with control group, Lnc-NTF3-5 expression in MIN6 cells was significantly increased in high glucose environment (p<0.05). In high glucose environment, Lnc-NTF3-5 plasmid transfection up-regulated Lnc-NTF3-5 expression, inhibited cell proliferation, increased Caspase 3 activity, and decreased SOD activity. Meanwhile, Lnc-NTF3-5 plasmid also increased ROS content and IL-1 level, and decreased IL-10 level and insulin secretion. Compared with high glucose group, the differences were statistically significant (p<0.05). However, transfection of Lnc-NTF3-5 siRNA down-regulated Lnc-NTF3-5 expression under high glucose environment and reversed the above changes. Compared with high glucose group, the differences were statistically significant (p<0.05)., Conclusions: Lnc-NTF3-5 expression is increased in high glucose environment. Targeting Lnc-NTF3-5 can inhibit islet cell apoptosis, oxidative stress, and promote islet cell proliferation and insulin secretion.
- Published
- 2019
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