1. Telmisartan attenuates kidney apoptosis and autophagy-related protein expression levels in an intermittent hypoxia mouse model.
- Author
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Zhang XB, Cai JH, Yang YY, Zeng YM, Zeng HQ, Wang M, Cheng X, Luo X, and Ewurum HC
- Subjects
- Angiotensin II blood, Animals, Blood Urea Nitrogen, Body Weight drug effects, Creatinine blood, Disease Models, Animal, Hypoxia pathology, In Situ Nick-End Labeling, Kidney pathology, Male, Mice, Mice, Inbred C57BL, Apoptosis drug effects, Autophagy-Related Proteins metabolism, Hypoxia physiopathology, Kidney drug effects, Telmisartan pharmacology
- Abstract
Purpose: Obstructive sleep apnea (OSA) is associated with renal impairs. As a novel pathophysiological hallmark of OSA, chronic intermittent hypoxia (CIH) enhances apoptosis and autophagy. The present study aims to evaluate the effect of telmisartan on CIH-induced kidney apoptosis and autophagy in a mouse model of OSA., Materials and Methods: Mice were randomly allocated to normoxia, CIH, and CIH+telmisartan groups (n = 12 in each group). The CIH exposure duration was 12 weeks. Mice in the CIH+telmisartan group received telmisartan administration. The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and western blotting of Bax and cleaved caspase-3 were conducted for evaluating apoptosis in kidney tissue. While the autophagy-related proteins, beclin-1 and LC3, were also observed via western blotting., Results: The percentage of apoptotic cell in the CIH group was significantly higher than that of normoxia group; meanwhile, Bax and cleaved caspase-3 protein levels were increased in the CIH group than those of normoxia group (all p < 0.05). Compared with the normoxia group, mice in the CIH group had greater autophagy-related proteins (beclin-1 and LC3) expression. When compared to the CIH group, both the renal apoptosis and autophagy in the CIH+telmisartan group were decreased., Conclusion: The CIH accelerates renal apoptosis and autophagy levels. Telmisartan ameliorating those levels suggests that it might prevent renal impairs from the CIH in OSA patients.
- Published
- 2019
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