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A disposable continuous-flow system was established by treating apple juice with induced electric field (IEF). Under synchronous alternating magnetic flux, rather than electrodes, the electrical treatment was implemented in multi-series coils consisting of the flowing sample. An activation effect at 50 °C and inactivation effect at 70 °C were observed under IEF. High excitation voltage (900 V) caused a significant decrease in the residual activities (RA). As the flow rate decreased from 87.5 to 17.5 mL/min, RA of PPO and POD were changed without applying IEF indicated shear effect of the pump had an impact on the RAs. At 17.5 mL/min, PPO was inactivated after IEF treatment for 7.6 min. In compared with the control (0 V), the fluid impedance was increased at 70 °C and 80 °C under IEF, whereas it was decreased at 30 °C and 40 °C. IEF had no adverse influence on the color and volatile compounds of apple juice in this system. [ABSTRACT FROM AUTHOR]

• A novel technique-Induced Electrical Field (IEF) for liquid sterilization was proposed. • The sterilization effects of IEF could be amplified with special structure. • The IEF treatment with moderate heat kept higher total phenol and reducing sugar. • A significant thermal effect and reduction of S. cerevisiae about 4.6 log (CFU/mL) were observed. The non-conventional technologies about continuous sterilization of liquid food were focused on recently, which is benefits for industrialization. In this study, the machine with an induced electric field was used to sterilize S. cerevisiae in apple juice and the juice quality also was researched. The optimal condition is 800 V, 400 Hz, 5 rpm and 2 mm. Furthermore, the sterilization of the IEF was attributed to non-thermal and thermal effects. The IEF treatment group has a reduction of about 4.6 logs (CFU/mL) in S. cerevisiae at 400 Hz, 800 V, and 2 mm, while the non-thermal group is nearly 2 logs (CFU/mL). The improvement of conductivity and the reduction of pH value imply that IEF might destroy the cell structure. Meanwhile, polyphenol compounds and amino acids in the IEF group were protected well than other groups. Generally, IEF is a potential technology for industrial sterilization of liquid beverages. [ABSTRACT FROM AUTHOR]

Non-conventional heating technology based on electric fields can be utilized to process liquid foods. In this study, the induced electric field (IEF) was investigated to clarify its inactivation mechanism on E.coli. Staining results show that inactivation of E.coli by IEF can be attributed to the reversible destruction of the cell membrane, followed by the denaturation of intracellular enzymes, and finally the irreversible rupture of the cell membrane. The increased levels of extracellular proteins and nucleic acids were also observed. IEF treatment at 400 Hz and 800 V (or 53 V/cm) results in a reduction of 4.5 log CFU·mL−1 in the number of E.coli. Storage life analysis shows that IEF treatment can improve the stability of apple juice and the content of bioactive components. Thus, IEF is a potential technique for liquid food processing. • The inactivation mechanism of induced electric field on E.coli was investigated. • A reduction of 4.5 log CFU·mL−1 of E.coli was realized by applying the IEF. • IEF treatment retains bio-active components in fresh apple juice. • The storage stability of apple juice was improved through IEF treatment. [ABSTRACT FROM AUTHOR]