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High-accuracy ship-based observations were conducted at 80°E in the Indian Ocean. Salinity below the mixed layer in 2019 was observed to be lower than that in 1995. This decrease in salinity was mainly attributed to anomalous advection associated with one of the strongest positive Indian Ocean dipole (pIOD) events in 2019 through analysis of the gridded time series of the salinity distributions based on the Argo float array. Increases and decreases in nitrate and dissolved inorganic carbon (DIC) and dissolved oxygen (DO), respectively, were also detected on the isopycnal surfaces where decreases in salinity were observed, suggesting that the anomalous upwelling and westward advection associated with the pIOD in the eastern part of the equatorial region resulted in low-salinity, low-oxygen, and nutrient-rich waters in the central off-equatorial region of the Indian Ocean. However, downward isopycnal heaving, which was also associated with the pIOD, was too strong to have increased nitrate below the mixed layers, and thus might have suppressed biological activity. The heaving also affected the DIC and DO distributions, and the effect of interannual changes such as those associated with the Indian Ocean dipole is essential to estimating changes in anthropogenic carbon storage. This research represents a case study, based on only two occupations; therefore, an assessment utilizing more intensive observations and more realistic numerical simulations is necessary in the future.