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Artificial reefs have been deployed in multiple regions of the world for different purposes including habitat restoration and protection, biodiversity and fish stock enhancement, fisheries management and recreation. Artificial reefs can be a valuable tool for ecosystem protection and rehabilitation, helping mitigate the effects of anthropogenic impacts that we face today. However, knowledge on artificial reefs is unevenly distributed worldwide, with some regions having much more quality information available and published (e.g. European Mediterranean Sea area), while others, for instance the North-East Atlantic area, do not. Here, we provide a characterization of purposely built artificial reefs in North-East Atlantic area based on all available literature (i.e. research papers and reports), highlighting the needs and gaps that are vital for establishing future perspectives for artificial reef deployment and research. In the North-East Atlantic area, sixty-one purposely built artificial reefs have been deployed since 1970, mostly between the years 1990–2009, with Spain being the country with the highest number of artificial reefs. The most reported purpose for their deployment is fisheries productivity and habitat/species protection, although, most artificial reefs are multipurpose in order to maximise the benefits of a given financial investment. The majority of artificial reefs were submerged at < 50 m, mainly between 10 and 20 m of depth. The most used designs were cubic blocks and complex designs made by an array of combined shapes, which mostly consist of concrete (79%). From all the analysed data on artificial reefs, 67% of the cases reported surveys to assess biodiversity after the deployment. However, in 26% of those cases, data was not available. When data was available, only 31% of cases reported long-term biomonitoring surveys (3 years or more). Based upon these findings, we noticed a general lack of scientifically robust data, including records of species and abundance of both fish and invertebrates, as well as macroalgae, preventing an adequate determination of the best balance between shape, construction material and bio-colonization. Critiques and suggestions are discussed in the light of currently available data in order to perform more efficient research, evaluation and functioning of future artificial reefs. info:eu-repo/semantics/publishedVersion

Recirculating aquaculture systems (RAS) have been developed to reduce the aquaculture environmental impact and control rearing conditions. They allow water reuse by managing waste and nutrient recycling, consequently making intensive fish production compatible with environmental sustainability. A key aspect of these systems is the water treatment performed by the beneficial bacterial community of the biofilter. In this study we aim to investigate the dynamics of the prokaryotic community of a sole (Solea senegalensis) hatchery RAS in relation to the variability of water physical-chemical parameters. Samples from different matrices (water, biofilter and tank wall biofilm) were collected from several compartments of a commercial RAS. Total DNA was isolated from the different matrices and the V4-V5 region of the 16S rRNA gene was sequenced using Illumina MiSeq® platform and the output analyzed in the DADA2 pipeline using the SILVAngs database. Overall, the prokaryotic communities were dominated by Proteobacteria (12–89%) and Bacteroidetes (8–86%) and a total of 58 genera contributed with more than 3% of the relative abundance across the different samples. The most abundant genera were Tenacibaculum, Sulfitobacter, Leucothrix, Novosphingobium, Marinicella, Pseudoalteromonas, Polaribacter_2, Schleiferia and Algibacter. The prokaryotic community shifts were found to be modelled by water parameters such as salinity and pH. This study provides new knowledge on the prokaryotic community composition in different units of recirculating systems, essential for the understanding of the microbial community balance in aquaculture and represents an important tool for overall aquaculture system management.

Hawksbill sea turtles are the most tropical sea turtle species with one of the last remaining aggregations in West Africa found on Príncipe Island (1° 37′ N; 7° 23′ E). Here we present for the first time, data on the trophic and foraging ecology of both juvenile and adult hawksbill turtles found in the shallow waters of Príncipe, using stable isotope analyses of carbon (δ13C) and nitrogen (δ15N), and assess any isotopic niche segregation between these two life-stages. Hawksbill turtles were sampled from December 2012 to June 2014, complementary to a snorkeling survey conducted around the entire coastline (ca. 100 km). δ13C and δ15N values were measured in epidermal samples of 40 individuals and analyzed in the context of isotopic niche width and overlap (i.e. trophic and foraging niches). Juveniles hawksbills (n = 29; < 60 cm curved carapace length (CCL)) occupy a significant smaller isotopic niche than adults (n = 11; > 75 cm), especially in relation to δ13C values (i.e. habitat use) but also in δ15N values (i.e. trophic level). Variances between adults and juveniles were not homogenous for both isotopes and larger for adults, particularly for δ13C values. We found significant differences in δ13C values between the two groups, but not for δ15N, and a significant correlation between δ13C values and CCL for the pooled data. SIBER (stable isotopes Bayesian ellipses in R) outcomes support these results and, although niches are not completely segregated, it indicates spatial foraging segregation between juveniles and adults. The fact that Príncipe Island's shallow waters are constantly being patrolled by spearfishermen, together with the existence of a large insular platform and a superior dive capacity in larger hawksbills, might influence this segregation. Our results suggest that part of the adults may be originating from isotopically distinct areas. To have a better understanding on the distribution and abundance of the sea turtle populations in Príncipe, including their connectivity with other foraging and breeding areas, future in-water studies focused mainly on the entire insular platform of Príncipe, but also on the adjacent probable foraging areas of the Gulf of Guinea, are required. info:eu-repo/semantics/publishedVersion

Difficulties and cost of suspended microalgal biomass harvest and processing can be overcome by cultivating microalgae as biofilms. In the present work, a new photoautotrophic biofilm photobioreactor, the rotating flat plate photobioreactor (RFPPB), was developed aiming at a cost-effective production of Chlorella vulgaris (SAG 211-12), a strain not frequently referred in the literature but promising for biofuel production. Protocols were developed for evaluating initial adhesion to different materials and testing the conditions for biofilm formation. Polyvinyl chloride substrate promoted higher adhesion and biofilm production, followed by polypropylene, polyethylene, and stainless steel. The new RFPPB was tested, aiming at optimizing incident light utilization, minimizing footprint area and simplifying biomass harvesting. Tests show that the photobioreactor is robust, promotes biofilm development, and has simple operation, small footprint, and easy biomass harvest. Biomass production (dry weight) under non-optimized conditions was 3.35 g m −2 , and areal productivity was 2.99 g m −2 day −1 . Lipid content was 10.3% (dw), with high PUFA content. These results are promising and can be improved by optimizing some operational parameters, together with evaluation of long-term photobioreactor maximum productivity. © 2017, Springer Science+Business Media B.V. This work was financially supported by the Project FCT UID/EQU/00305/2013 and Project POCI-01-0145-FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by FEDER funds through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI)—and by national funds through FCT— Fundação para a Ciência e a Tecnologia—and partially supported also by the Strategic Funding UID/Multi/04423/2013 through national funds provided to CIIMAR (Interdisciplinary Centre of Marine and Environmental Research) by FCT and European Regional Development Fund (ERDF), in the framework of the program PT2020.

Direct and continuous measurement of dissolved CO2 (dCO2) is crucial for intensive aquaculture, especially in shallow raceway systems (SRS). In this work the performance of a portable dissolved CO2 probe analyzer was tested for the effects of different aqueous solutions, pure oxygen injection and agitation. Laboratory results showed significant (p

This study provides the first description of the feeding ecology of the smooth lanternshark Etmopterus pusillus based on stomach contents of specimens caught as bycatch in the Algarve (southern Portugal) with bottom trawling and bottom longline. The diet of E. pusillus consists mainly of fish (dry weight (% W)=87.1%; frequency of occurrence (%FO)=28.6%; number (%N)=30.3%), crustaceans (%W=7.7%; %FO=36.7%; %N=3.4%) and cephalopods (%W=4.7%; %FO=11.3%; %N=11.1%). The diet did not vary between sexes. Ontogenic changes were detected: crustaceans decreased in importance as the sharks increased in size and fish became dominant in the diet of adults. Combining two fishing methods provided broad information on the diet of E. pusillus, as bottom trawling caught smaller specimens and longlines caught larger individuals. E. pusillus feeds mainly on non-commercial species, and therefore does not compete directly with commercial fisheries. Finally, E. pusillus feeds in various parts of the water column and thus it can access a wide range of prey; however, this also means that it can be caught by both gears, making it more vulnerable in terms of conservation.

Information on commercial aquaculture systems bacterial communities is scarce. The present study was performed in a turbot shallow raceway recirculating aquaculture system throughout 221 days after initial fish stocking. The dynamics of bacteria attached to a Moving Bed Biofilm Reactor and the distribution of the system culturable planktonic heterotrophic bacteria were assessed. Different levels of free heterotrophic bacteria were found over time: ≤2.6 × 104 CFU mL−1 in incoming and ozonated water and ≥1.5 × 106 CFU mL−1 in raceway and biofilter water. 16S rRNA gene sequencing of nine strains persistently isolated from the system affiliated with Bacteroidetes, α-Proteobacteria or γ-Proteobacteria. Polymerase chain reaction-denaturating gradient gel electrophoresis (PCR-DGGE) data showed temporal and spatial segregation of attached biofilter community. The first 32 days were the most important for bacterial establishment with higher culturable bacteria biomass loadings and higher community diversity. Multivariate canonical correspondence analysis of PCR-DGGE showed that the most common aquaculture water quality descriptors explained 70% of the DGGE pattern variability.

In order to understand how marine ecosystems function, it is essential to study the trophic interactions among the community members, particularly from poorly known regions. In this study, the feeding ecology and diet of conger eels,Conger conger, an abundant fish species with commercial interest, was examined in the north-east Atlantic, off Algarve (southern Portugal) between May 2005 and August 2006. The diet was characterized by species composition, size and mass of prey. Conger eels are opportunistic feeders, cannibalistic, feeding on benthopelagic/pelagic prey (67% by mass and 71% by number) but also benthic prey (32% by mass and 29% by number). Fish (67.8±4.7% in mass) are the main prey of conger eels, followed by cephalopods (16.5±3.8%) and crustaceans (15.6±3.7%). The most numerous (identifiable) fish consumed wereCapros aper, occurring in 90% of the stomach samples that contained food and representing 1.4% in mass, andScomber japonicus, the most important fish in mass (21.1%), which occurred in 4.2% of the stomach samples that contained food. The present study shows that octopodids can play a more important role in the diet of conger eels than previously thought. Of the species preyed upon by conger eels, six species (21% of the total prey taxa) are caught commercially(Trachurus trachurus, Scomber japonicus, Micromesistius poutassou, Helicolenus dactylopterusandConger conger)by local fisheries. As discards by local trawl and longline fisheries do not correspond with the diet ofC. conger, it is likely that most prey ofC. congerin rocky areas were caught actively in that study region.

The effects of marine fishing activities on benthic habitat and communities have become an important environmental issue. In addition to the direct removal of target species, effects include by-catch, damage to benthic organisms and alteration of habitat structure. The growing number of studies on the impact of fishing on bottom habitats indicates that the effects vary with the physical nature of the seabed and with the local natural disturbance regime. Several studies have been conducted on fisheries by-catch and discards off the south coast of Portugal since 1996. The results provide an idea of the impact on biodiversity: more than 60% of the species caught by the trawl fishery are discarded. The crustacean trawl captures the greatest number of species, probably due to the greater fishing depth range. In April 2004, the manned submersible ‘Delta’ (from DELTA Oceanographics, USA) conducted a sea campaign integrated in the SEMAPP programme (Science, Education, and Marine Archaeology Program in Portugal) to directly observe and assess the biological, geological, and archaeological aspects. A total of 15 dives were conducted mainly near the head and in the flanks inside Portimao canyon, down to 300 m. Observations showed variations in bottom type, the sedimentary framework and biological communities. These dives (150–300 m depth) also revealed a heavily trawled canyon bottom and erosion structures leading to changes in habitat structure and biodiversity. Of special interest was the occurrence of low-relief boulder substrates with relatively high densities of demersal finfish and shellfish. These sites served as a refuge for several species, whose abundance here was greater than on the surrounding areas of fine-grained substrates. Strong marks on the bottom are apparently caused by the doors of the trawl nets. Their impact will be examined in greater detail in future studies.

The diet of Sepia officinalis was studied by examining the stomach contents of 522 specimens caught off the coast of the Algarve, Portugal. A total of 49 different prey items, belonging to six taxa (Polychaeta, Cephalopoda, Teleostei, Bivalvia, Crustacea and Gastropoda) was found, indicating opportunistic feeding behaviour. The diet was examined in relation to season, sex and size. The diet of males and females were similar. For small S. officinalis, crustaceans were more important in weight, whereas for larger specimens, fish were dominant. Seasonal differences in the diet were apparent but were shown to be possibly influenced by the cuttlefish size.

The effects of commercial trawling on the malacological communities (except for the Cephalopoda) were examined, based on a study undertaken between 1996 and 2000 on the continental shelf and slope of southern Portugal. More than 50% of species caught by trawling in southern Portugal were discarded, with molluscs representing about 19%. Forty-four species of molluscs (15 bivalves, 28 gastropods and one polyplacophoran) were identified from the discarded specimens. Crustacean trawlers accounted for 34 molluscs species, and fish trawlers for 24. Twenty species were only caught by the crustacean trawl, compared with 10 species by the fish trawl, and 14 species were common to both trawls. The bivalve Venus nux Gmelin was the most numerous species discarded in the fish trawl, accounting for 42.0% of the total number of individuals, followed by the gastropods Ampulla priamus (Meuschen) (7.8%) and Ranella olearium (L.) (7.3%). In the crustacean trawl, the most numerous species discarded were the bivalve Anadara diluvii (Lamarck) (19.4%), the gastropod Calliostoma granulatum (Born) (15.5%), and the bivalve V. nux (15.1%). The third most discarded species from fish trawls in Algarve waters, the gastropod species R. olearium, is a species listed in Annex II of the Bern Convention. The difficulties of managing the real impact of fisheries on the molluscan populations and in defining a conservation strategy are discussed.

EU regulations recommend effluent treatment and nutrient recycling for aquaculture sustainability, so a study was undertaken to provide base-line data for the integration of commercial fish-farm effluents with the production of microalgae. The project relates to a specific bivalve consumer (Tapes decussatus) and biofiltration. Effluent inorganic nutrient composition was assessed and evaluated as culture media for Phaeodactylum tricornutum, Tetraselmis suecica and Tetraselmis sp. Optimization of the microalgal compartment included studies on preparation of a simple medium, nutrient or dilution rate manipulation and nutrient removal. Cell harvest was increased chiefly by N correction (6-fold for Tetraselmis sp.) and semi-continuous or continuous operation (by a factor of 3 to 11). Nutrient removal efficiency was high for ammonium and nitrite-nitrogen (80–100%), depending on species, nutrient ratio (Si correction for P. tricornutum) and culture regime for nitrate (41–100%) or phosphorus (21–99%). Data obtained under cyclostat cultivation (yields of 1.38 and 0.50×106 P. tricornutum or Tetraselmis sp. cells mL−1 d−1 and nutrient uptake rates of 2.32 mg N L−1 d−1 and 0.96 mg P L−1 d−1) were used to show clam production and simultaneous wastewater treatment possibilities through the proposed fish-microalgae-clam integrated aquaculture system.

By-catch and discards are a common problem to all fisheries. Recent studies of the Portuguese crustacean-trawl fishery show that discards average 70% of the total catch. This situation poses a threat not only to the management of target and commercial fish by-catch species stocks, but also in terms of the ecosystem impact of the discarded species whose survival is generally low. Previous attempts to address this problem by using oblique separator mesh panels associated with square-mesh windows, or square-mesh windows alone, provided encouraging results, but these are unlikely to have commercial applicability at present. Therefore, we tested the efficiency of a modified Nordmore grid in excluding some of the most captured non-commercial by-catch species, such as the blue whiting, Micromesistius poutassou, and the boarfish, Capros aper, evaluating simultaneously the losses of the crustacean target species (rose shrimp, Parapenaeus longirostris, Norway lobster, Nephrops norvegicus and red shrimp, Aristeus antennatus) and commercially valuable fish by-catch. A total of 41 valid hauls were carried out during 2001, and 15 in 2002 on board the R/V "Noruega". Results from both years varied considerably, with higher by-catch exclusion rates, in weight, registered in the second survey (73-74 and 48-63% for blue whiting and boarfish, respectively). The corresponding percentage reduction for target species was 8-15% for the Norway lobster, 4-9% for rose shrimp and 7-10% for red shrimp. The level of exclusion of the non-commercial by-catch is encouraging, although short-term loss of Norway lobster, where the escapees comprised larger individuals, raises some concern with regards to fishers' acceptance of mandatory use of grids. (C) 2004 Elsevier B.V. All rights reserved.

Discards from five of the most important fisheries (crustacean trawling, fish trawling, demersal purse seining, pelagic purse seining and trammel netting) in southern Portugal were studied and compared. A total of 236 species of all taxa were discarded, with fish and cephalopods accounting for more than 90% of the discarded biomass, except trammel nets (81%). Although there was some overlapping of species, multivariate analysis using cluster analysis for classification and multidimensional scaling (MDS) for ordination, showed that there were significant differences between the five gears in terms of species composition and biomasses discarded, with the least similarity between crustacean trawling and all other fishing operations. The differences between fisheries were probably as a result of a combination of gear selectivity and depth fished. The results suggest that discarding practices are likely to have different, yet significant impacts on the marine ecosystem, warranting further studies on the fate of discards, the factors influencing discarding and mitigation.

A preliminary investigation was made of the trophic relationships of three species of cartilaginous fish and 12 species of bony fishes along the upper part of the continental slope off the Algarve. These fish, from the by-catch of crustacean bottom trawlers fishing between 190 and 650 m, were sampled for stomach content analysis. Three major predator assemblages were identified. Group 1 consumers preyed upon zooplankton, particularly euphausids, and micronekton usually associated with the deep scattering layers. Group 2 consumers were larger predators that fed largely on fish, particularly those of group 1, and several penaeid and pandalid shrimps. Group 3 consumers had a stronger benthic affinity, and their most important prey items were isopods, followed by crabs and shrimps. The euphausid Meganyctiphanes norvegica seems to play a key role in this food web. The results are discussed from a biogeographic perspective. The deep-water fishery off Algarve seems to be characterised by large technological (multi-fleet) and biological (multi-species) interactions.

Summary Gurnards or triglids are medium-size marine bottom fishes that live in tropical and temperate seas. Four genera (Aspitrigla, Chelidonichthys, Lepidotrigla, Trigla) and eight species are found in the eastern Atlantic. In this study, we report the weight–length relationships for eight species, with 75 specimens of A. cuculus, 45 of C. obscurus, 84 C. gurnardus, 21 C. lucernus, 45 C. lastoviza, 550 L. cavillone, 255 L. dieuzeidei and 40 T. lyra. These triglids were caught by three different methods: crustacean trawl, fish trawl and purse seine off the Algarve coast; all specimens sampled were part of the discards. Samples were obtained from depths ranging between 20 and 540 m, during 27 trips and 86 hauls. To the best knowledge of the authors, this study presents the first reference on weight–length relationships for four fish species from the Algarve coast (southern Portugal).

Four 100 m lengths of both monofilament gill nets and trammel nets were deployed at depths between 15 and 18 m off the coast of the Algarve (south of Portugal) between April 1995 and June 1996. The nets were set on a natural rocky bottom with one end cut loose to simulate lost nets. Changes in net structure (net height, effective fishing area, movement, colonisation, wear and tear) and their catches (species, sizes, numbers, and biomass) were monitored by divers. Similar patterns were observed in all the nets, with a sharp decrease in net height and effective fishing area, and an increase in visibility within the first few weeks. Net movement was negligible except in the case of interference from other fishing gears. Catch rates were initially comparable to normally fished gill nets and trammel nets in this area, but decreased steadily over time. No sea birds, reptiles or mammals were caught in any of the 8 nets. Catches were dominated by fish (89 % by number, at least 27 species), in particular by sea breams (Sparidae) and wrasses (Labridae). Under the conditions experienced throughout the study the fishing Lifetime of a 'lost' net is between 15 and 20 wk. Based on an exponential model, we estimated that 100 m lengths of gill net and trammel net will catch 314 and 221 fish respectively over a 17 wk period. However, we consider this to be an underestimate due to high rates of predation and scavenging by octopuses, cuttlefish, moray eels, conger eels, and other fish such as the wrasse Coris julis. When the nets were surveyed in the following spring, 8 to 11 mo after being deployed, they were found to be completely destroyed or heavily colonised by algae and had become incorporated into the reef.

Summary Weight and length descriptive statistics are reported for 73 fish species discarded in the commercial fisheries from the south coast of Portugal. Parameters of the weight–length relationship were estimated for 57 of these species. These are the first weight–length parameters reported for 20 of these species. For an additional 16 species for which no weight–length parameters have been reported, the parameter a was estimated by setting b = 3.0. Samples were used from five gear types: crustacean trawler, fish trawler, pelagic purse seine, demersal purse seine and trammel net. Samples were obtained from depths ranging from 500 m and included pelagic and demersal species.

As part of two research projects for analysing bycatch and discards, we quantified catch composition, catch rates, bycatch and discards in two important commercial bottom trawl fisheries (crustacean and fish trawls) off the southern coast of Portugal (Algarve). Stratified sampling by onboard observers took place from February 1999 to March 2001 and data were collected from 165 tows during 52 fishing trips. Commercial target species included crustaceans: blue and red shrimp (Aristeus antennatus), deep-water rose shrimp (Parapenaeus longirostris), Norway lobster (Nephrops norvegicus); and fishes: seabreams (Diplodus spp. and Pagellus spp.), horse mackerels (Trachurus spp.) and European hake (Merluccius merluccius). The trawl fisheries are characterised by considerable amounts of bycatch: 59.5% and 80.4% of the overall total catch for crustacean and fish trawlers respectively. A total of 255 species were identified, which belonged to 15 classes of organisms (137 vertebrates, 112 invertebrates and 6 algae). Crustacean trawlers had higher bycatch biodiversity. Bony fish (45.6% and 37.8%) followed by crustaceans (14.6% and 11.5%) were the dominant bycatch components of both crustacean and fish trawlers respectively. The influence of a number of factors (e.g. depth, fishing gear, tow duration and season) on bycatch and discards is discussed., Se ha cuantificado la composición de las capturas, sus tasas, las capturas incidentales y los descartes de dos pesquerías industriales de arrastre de fondo dirigidas a crustáceos y peces del sur del Portugal (Algarve). Se realizó un muestreo estratificado mediante observadores a bordo desde febrero de 1999 hasta marzo de 2001, utilizándose los datos recogidos en 52 embarques y 165 lances. Las especies objetivos de la pesquería comercial fueron: la gamba roja (Aristeus antennatus), la gamba blanca (Parapennaeus longirostris), la cigala (Nephrops norvegicus), varias especies de espáridos de los géneros Diplodus y Pagellus, jureles (Trachurus spp.) y merluza (Merluccius merluccius). Estas pesquerías de arrastre de fondo se caracterizaron por la presencia de considerables cantidades de capturas incidentales, que variaron entre un 59.5 y un 80.4% del total de las capturas de crustáceos y peces, respectivamente. Se identificaron 255 especies pertenecientes a 15 clases de organismos (137 vertebrados, 112 invertebrados y 6 algas). Se observó que la mayor biodiversidad se produjo en los arrastreros dirigidos a la captura de crustáceos. El componente dominante de las capturas incidentales en los arrastreros dirigidos a crustáceos y peces fueron los teleósteos (45.6 y 37.8%, respectivamente), seguidos por los crustáceos (14.6 y 11.5%). Se discute la influencia de varios factores (i.e. la profundidad, el tipo de aparejo, la duración del lance o la estación del año) en las capturas incidentales y los descartes.

the reproductive biology of 1045 female (9.5–67 cm (total length (tl)) and 1007 male (9.4–64.3 cm tl) blackmouth catsharks, galeus melastomus, was investigated. the sharks were caught off southern portugal by bottom crustacean trawlers at depths from 209 to 754 m. the sex ratio was 1:1, and this species is sexually dimorphic with males approaching maturity at smaller size than females. sexual segregation appears to be given for the stock within the study area. sexual maturity was reached at a total length above 49 cm in males and above 56 cm in females. mating and egg-deposition take place all year round, with two reproductive peaks of activity, in winter and summer. egg capsules are, on average, 54 mm long and 21 mm wide, with a maximum of 63×25 mm encountered. morphometric measurements of claspers, testes, ovaries, and oviducal glands were suitable for determining sexual maturity of blackmouth catshark.

Water treatment systems are mandatory in recirculating aquaculture facilities facing existing regulations, but data on system efficiency, especially for marine species, are scarce. The present work aimed at contributing to the evaluation of the effluent characteristics and the performance of a combined outdoor biological and non-biological treatment system in an intensive turbot (Scophthalmus maximus) farm, operating under different hydraulic regimes. A preliminary study on the biofilter bacterial populations was also undertaken. Changes in effluent characteristics with pumping, season of the year and fish biomass were observed. The treatment system showed performance instability under the conditions assayed (outdoors, changeable recycle rates). Maximum removal of solids was observed in winter, with microscreen or biological filtration (up to 60%) and nitrite removal (40-98%) was achieved with ozonation. Reduction in ammonium levels was higher in summer, either mechanically (74%) or biologically (33%). Phosphate removal was higher in winter with both systems (37 and 60%, respectively). Compliance with Portuguese discharge standards was achieved. For improvements in the treatment loop, further studies on biofilter bacteria under outdoor conditions are needed, and biological denitrification is encouraged.

The first occurrence of a giant squid Architeuthis sp. specimen in Portuguese waters is noted and another reference set of biological and biometrical data provided which may in future be used in conjunction with others to further elucidate important aspects of the identity, biology and ecology of the species. The fact that the specimen is a male is relevant, since males have been much less frequent in the reports in the scientific literature than have females. Additionally, this is the most southerly report of a male known to have occurred in the Atlantic Ocean.

In this study, we investigated the effect of simulated salinities in the nitrification process in estuarine sediments through fingerprinting analysis of ammonia-oxidizing archaea (AOA) and Betaproteobacteria (β-AOB) amoA genes transcription, isotopic measurements of total and selective (AOA and AOB) nitrification rates, and inorganic nitrogen fluxes.Results revealed that ammonia oxidizers adapted to distinct estuarine salinity regimes exhibited different responses in terms of total nitrification activity when facing salinity changes of 0, 15 and 30, with the downstream sediments displaying higher plasticity towards salinity increment (0.058, 0.117 and 0.123 nmol N g-1 h-1 mL-1, respectively), while the upstream sediments displayed a decrease in potential nitrification rates as a consequence of salinity increment (0.472, 0.378, 0.179 nmol N g-1 h-1 mL-1, respectively). In addition, our findings demonstrated that salinity is an important variable in controlling the selection of the group of organisms (archaea or bacteria ammonia oxidizers) that mediated the nitrification process in estuarine systems. We believe that results from this study are critical to understand the response of estuarine nitrifier communities to different salinity regimes. © 2017 Elsevier. This research was partially supported by the Structured Program of R&D&I CORAL - NORTE-01-0145-FEDER-000036 and MarInfo - NORTE-01-0145-FEDER-000031, funded by the NORTE2020 through the European Regional Development Fund (ERDF) . The authors also acknowledge the Portuguese Science and Technology Foundation (FCT) for the financial support through the ERA-NET COFASP project Ref. COFASP/0005/2015 , and through the fellowship SFRH/BPD/104466/2014 .