1. Design and performance of a dark‐field probe with confocal Raman spectroscopy for ophthalmic applications.
- Author
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Zhang, Shuo, Erckens, Roel J., Jongsma, Franciscus H.M., Webers, Carroll A.B., and Berendschot, Tos T.J.M.
- Subjects
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AQUEOUS humor , *FUSED silica , *ARTIFICIAL eyes , *RAY tracing , *PHENYLEPHRINE , *LATTICE dynamics - Abstract
A dark‐field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36‐fold improvement in the signal‐to‐noise ratio at the phenylephrine HCl 1002 cm−1 peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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