1. The canonical α-SNAP is essential for gametophytic development in Arabidopsis.
- Author
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Liu F, Li JP, Li LS, Liu Q, Li SW, Song ML, Li S, and Zhang Y
- Subjects
- ATPases Associated with Diverse Cellular Activities genetics, Alternative Splicing genetics, Arabidopsis growth & development, Germ Cells, Plant growth & development, Mitosis genetics, N-Ethylmaleimide-Sensitive Proteins genetics, Protein Isoforms genetics, Arabidopsis genetics, Gametogenesis genetics, Plant Development genetics, Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins genetics
- Abstract
The development of male and female gametophytes is a pre-requisite for successful reproduction of angiosperms. Factors mediating vesicular trafficking are among the key regulators controlling gametophytic development. Fusion between vesicles and target membranes requires the assembly of a fusogenic soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) complex, whose disassembly in turn ensures the recycle of individual SNARE components. The disassembly of post-fusion SNARE complexes is controlled by the AAA+ ATPase N-ethylmaleimide-sensitive factor (Sec18/NSF) and soluble NSF attachment protein (Sec17/α-SNAP) in yeast and metazoans. Although non-canonical α-SNAPs have been functionally characterized in soybeans, the biological function of canonical α-SNAPs has yet to be demonstrated in plants. We report here that the canonical α-SNAP in Arabidopsis is essential for male and female gametophytic development. Functional loss of the canonical α-SNAP in Arabidopsis results in gametophytic lethality by arresting the first mitosis during gametogenesis. We further show that Arabidopsis α-SNAP encodes two isoforms due to alternative splicing. Both isoforms interact with the Arabidopsis homolog of NSF whereas have distinct subcellular localizations. The presence of similar alternative splicing of human α-SNAP indicates that functional distinction of two α-SNAP isoforms is evolutionarily conserved., Competing Interests: The authors declare no conflict of interests.
- Published
- 2021
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