1. Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance.
- Author
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Ramu VS, Dawane A, Lee S, Oh S, Lee HK, Sun L, Senthil-Kumar M, and Mysore KS
- Subjects
- Arabidopsis immunology, Arabidopsis microbiology, Arabidopsis Proteins genetics, Disease Resistance genetics, Gene Expression, Mutation, Plant Diseases microbiology, Protein Biosynthesis, RNA Interference, Ribosomal Protein L10 genetics, Ribosomal Proteins genetics, Ribosomes metabolism, Sequence Analysis, RNA, Nicotiana genetics, Nicotiana immunology, Nicotiana microbiology, Two-Hybrid System Techniques, Arabidopsis genetics, Arabidopsis Proteins metabolism, Solanum lycopersicum microbiology, Plant Diseases immunology, Pseudomonas syringae pathogenicity, Ribosomal Protein L10 metabolism, Ribosomal Proteins metabolism
- Abstract
Ribosomes play an integral part in plant growth, development, and defence responses. We report here the role of ribosomal protein large (RPL) subunit QM/RPL10 in nonhost disease resistance. The RPL10-silenced Nicotiana benthamiana plants showed compromised disease resistance against nonhost pathogen Pseudomonas syringae pv. tomato T1. The RNA-sequencing analysis revealed that many genes involved in defence and protein translation mechanisms were differentially affected due to silencing of NbRPL10. Arabidopsis AtRPL10 RNAi and rpl10 mutant lines showed compromised nonhost disease resistance to P. syringae pv. tomato T1 and P. syringae pv. tabaci. Overexpression of AtRPL10A in Arabidopsis resulted in reduced susceptibility against host pathogen P. syringae pv. tomato DC3000. RPL10 interacts with the RNA recognition motif protein and ribosomal proteins RPL30, RPL23, and RPS30 in the yeast two-hybrid assay. Silencing or mutants of genes encoding these RPL10-interacting proteins in N. benthamiana or Arabidopsis, respectively, also showed compromised disease resistance to nonhost pathogens. These results suggest that QM/RPL10 positively regulates the defence and translation-associated genes during nonhost pathogen infection., (© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
- Published
- 2020
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