1. The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production.
- Author
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Zhou HR, Lin RN, Huang HW, Li L, Cai T, Zhu JK, Chen S, and He XJ
- Subjects
- Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis Proteins physiology, DNA-Directed RNA Polymerases physiology, Repressor Proteins genetics, Repressor Proteins physiology, Transcription Factors physiology, Arabidopsis Proteins metabolism, DNA Methylation, DNA-Directed RNA Polymerases metabolism, RNA, Small Interfering metabolism, Repressor Proteins metabolism, Transcription Factors metabolism
- Abstract
Small interfering RNAs (siRNAs) are responsible for establishing and maintaining DNA methylation through the RNA-directed DNA methylation (RdDM) pathway in plants. Although siRNA biogenesis is well known, it is relatively unclear about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana, we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. We demonstrated that NOT1 is required for Pol IV-dependent siRNA accumulation and DNA methylation at a subset of RdDM target genomic loci. Furthermore, we revealed that NOT1 is a constituent of a multi-subunit CCR4-NOT deadenylase complex by immunoprecipitation combined with mass spectrometry and demonstrated that the CCR4-NOT components can function as a whole to mediate chromatin silencing. Therefore, our work establishes that the CCR4-NOT complex regulates the biogenesis of Pol IV-dependent siRNAs, and hence facilitates DNA methylation and transcriptional silencing in Arabidopsis., (© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.)
- Published
- 2020
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