17 results on '"Zabotina, Olga A."'
Search Results
2. The Arabidopsis xylosyltransferases, XXT3, XXT4, and XXT5, are essential to complete the fully xylosylated glucan backbone XXXG‐type structure of xyloglucans.
- Author
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Zhang, Ning, Julian, Jordan D., Yap, Cheng Ern, Swaminathan, Sivakumar, and Zabotina, Olga A.
- Subjects
XYLOGLUCANS ,PLANT cell walls ,ARABIDOPSIS ,ARABIDOPSIS thaliana ,SPINE ,GLUCANS ,CRISPRS - Abstract
Summary: Although most xyloglucans (XyGs) biosynthesis enzymes have been identified, the molecular mechanism that defines XyG branching patterns is unclear. Four out of five XyG xylosyltransferases (XXT1, XXT2, XXT4, and XXT5) are known to add the xylosyl residue from UDP‐xylose onto a glucan backbone chain; however, the function of XXT3 has yet to be demonstrated.Single xxt3 and triple xxt3xxt4xxt5 mutant Arabidopsis (Arabidopsis thaliana) plants were generated using CRISPR‐Cas9 technology to determine the specific function of XXT3.Combined biochemical, bioinformatic, and morphological data conclusively established for the first time that XXT3, together with XXT4 and XXT5, adds xylosyl residue specifically at the third glucose in the glucan chain to synthesize XXXG‐type XyGs. We propose that the specificity of XXT3, XXT4, and XXT5 is directed toward the prior synthesis of the acceptor substrate by the other two enzymes, XXT1 and XXT2. We also conclude that XXT5 plays a dominant role in the synthesis of XXXG‐type XyGs, while XXT3 and XXT4 complementarily contribute their activities in a tissue‐specific manner.The newly generated xxt3xxt4xxt5 mutant produces only XXGG‐type XyGs, which further helps to understand the impact of structurally deficient polysaccharides on plant cell wall organization, growth, and development. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component
- Author
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Cavalier, David M., Lerouxel, Olivier, Neumetzler, Lutz, Yamauchi, Kazuchika, Reinecke, Antje, Freshour, Glenn, Zabotina, Olga A., Hahn, Michael G., Burgert, Ingo, Pauly, Markus, Raikhel, Natasha V., and Keegstra, Kenneth
- Subjects
Arabidopsis thaliana ,Cellulose ,Plant genetics ,Biological sciences ,Science and technology - Published
- 2008
4. Re‐targeting of a plant defense protease by a cyst nematode effector.
- Author
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Pogorelko, Gennady V., Juvale, Parijat S., Rutter, William B., Hütten, Marion, Maier, Thomas R., Hewezi, Tarek, Paulus, Judith, der Hoorn, Renier AL, Grundler, Florian MW, Siddique, Shahid, Lionetti, Vincenzo, Zabotina, Olga A., and Baum, Thomas J.
- Subjects
PLANT defenses ,SUGAR beet cyst nematode ,BOTRYTIS cinerea ,CARBOHYDRATE metabolism ,ARABIDOPSIS thaliana - Abstract
Summary: Plants mount defense responses during pathogen attacks, and robust host defense suppression by pathogen effector proteins is essential for infection success. 4E02 is an effector of the sugar beet cyst nematode Heterodera schachtii. Arabidopsis thaliana lines expressing the effector‐coding sequence showed altered expression levels of defense response genes, as well as higher susceptibility to both the biotroph H. schachtii and the necrotroph Botrytis cinerea, indicating a potential suppression of defenses by 4E02. Yeast two‐hybrid analyses showed that 4E02 targets A. thaliana vacuolar papain‐like cysteine protease (PLCP) 'Responsive to Dehydration 21A' (RD21A), which has been shown to function in the plant defense response. Activity‐based protein profiling analyses documented that the in planta presence of 4E02 does not impede enzymatic activity of RD21A. Instead, 4E02 mediates a re‐localization of this protease from the vacuole to the nucleus and cytoplasm, which is likely to prevent the protease from performing its defense function and at the same time, brings it in contact with novel substrates. Yeast two‐hybrid analyses showed that RD21A interacts with multiple host proteins including enzymes involved in defense responses as well as carbohydrate metabolism. In support of a role in carbohydrate metabolism of RD21A after its effector‐mediated re‐localization, we observed cell wall compositional changes in 4E02 expressing A. thaliana lines. Collectively, our study shows that 4E02 removes RD21A from its defense‐inducing pathway and repurposes this enzyme by targeting the active protease to different cell compartments. Significance Statement: The 4E02 effector of the sugar beet cyst nematode Heterodera schachtii targets Arabidopsis thaliana vacuolar PLCP RD21A, which functions in the plant defense response. 4E02 mediates a re‐localization of this protease to the nucleus and cytoplasm, which is accompanied by altered expression levels of defense response genes, higher susceptibility to H. schachtii and Botrytis cinerea, and cell wall compositional changes. These findings uncover mechanistic details in the parasitic strategy of a damaging pathogen, which could be exploited in future control strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
5. Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
- Author
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Pogorelko, Gennady V., Reem, Nathan T., Young, Zachary T., Chambers, Lauran, and Zabotina, Olga A.
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ASPERGILLUS nidulans ,FUCOSIDASES ,ARABIDOPSIS ,PLANT cell walls ,FUCOSYLTRANSFERASES ,PLANT growth ,CELL communication ,PLANT-microbe relationships - Abstract
Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant. Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth. The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes. Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations. Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes. These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
6. Decreased Polysaccharide Feruloylation Compromises Plant Cell Wall Integrity and Increases Susceptibility to Necrotrophic Fungal Pathogens.
- Author
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Reem, Nathan T., Pogorelko, Gennady, Lionetti, Vincenzo, Chambers, Lauran, Held, Michael A., Bellincampi, Daniela, Zabotina, Olga A., Misra, Biswapriya Biswavas, and Pasternak, Taras P.
- Subjects
POLYSACCHARIDES ,PHYTOPATHOGENIC microorganisms ,FERULIC acid - Abstract
The complexity of cell wall composition and structure determines the strength, flexibility, and function of the primary cell wall in plants. However, the contribution of the various components to cell wall integrity (CWI) and function remains unclear. Modifications of cell wall composition can induce plant responses known as CWI control. In this study, we used transgenic expression of the fungal feruloyl esterase AnFAE to examine the effect of post-synthetic modification of Arabidopsis and Brachypodium cell walls. Transgenic Arabidopsis plants expressing AnFAE showed a significant reduction of monomeric ferulic acid, decreased amounts of wall-associated extensins, and increased susceptibility to Botrytis cinerea, compared with wild type. Transgenic Brachypodium showed reductions in monomeric and dimeric ferulic acids and increased susceptibility to Bipolaris sorokiniana. Upon infection, transgenic Arabidopsis and Brachypodium plants also showed increased expression of several defense-related genes compared with wild type. These results demonstrate a role, in both monocot and dicot plants, of polysaccharide feruloylation in plant CWI, which contributes to plant resistance to necrotrophic pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
7. Protein–Protein Interactions Among Xyloglucan-Synthesizing Enzymes and Formation of Golgi-Localized Multiprotein Complexes.
- Author
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Chou, Yi-Hsiang, Pogorelko, Gennady, Young, Zachary T., and Zabotina, Olga A.
- Subjects
PROTEIN-protein interactions ,XYLOGLUCANS ,CHEMICAL synthesis ,ENZYMES ,GOLGI apparatus ,ARABIDOPSIS thaliana ,CELLULOSE synthase - Abstract
Arabidopsis thaliana xyloglucan has an XXXG structure, with branches of xylosyl residues, β-d-galacosyl-(1,2)-α-d-xylosyl motifs and fucosylated β-d-galactosyl-(1,2)-α-d-xylosyl motifs. Most of the enzymes involved in xyloglucan biosynthesis in Arabidopsis have been identified, including the glucan synthase CSLC4 (cellulose synthase-like C4), three xylosyltransferases (XXT1, XXT2 and XXT5), two galactosyltransferases (MUR3 and XLT2) and the fucosyltransferase FUT1. The XXTs and CSLC4 form homo- and heterocomplexes and were proposed to co-localize in the same complex, but the organization of the other xyloglucan-synthesizing enzymes remains unclear. Here we investigate whether the glycosyltransferases MUR3, XLT2 and FUT1 interact with the XXT–CSLC4 complexes in the Arabidopsis Golgi. We used co-immunoprecipitation and bimolecular fluorescence complementation, with signal quantification by flow cytometry, to demonstrate that CSLC4 interacts with MUR3, XLT2 and FUT1. FUT1 forms homocomplexes and interacts with MUR3, XLT2, XXT2 and XXT5. XLT2 interacts with XXT2 and XXT5, but MUR3 does not. Co-immunoprecipitation assays showed that FUT1 forms a homocomplex through disulfide bonds, and formation of the heterocomplexes does not involve covalent interactions. In vitro pull-down assays indicated that interactions in the FUT1–MUR3 and FUT1–XXT2 complexes occur through the protein catalytic domains. We propose that enzymes involved in xyloglucan biosynthesis are functionally organized in multiprotein complexes localized in the Golgi. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
8. Pectin–Cellulose Interactions in the Arabidopsis Primary Cell Wall from Two-Dimensional Magic-Angle-Spinning Solid-State Nuclear Magnetic Resonance.
- Author
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Tuo Wang, Zabotina, Olga, and Mei Hong
- Subjects
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ARABIDOPSIS thaliana , *PLANT cell walls , *PECTINS , *CELLULOSE , *POLYSACCHARIDES , *NUCLEAR magnetic resonance spectroscopy , *SPECTRUM analysis , *CHEMICAL shift (Nuclear magnetic resonance) - Abstract
The primary cell wall of higher plants consists of a mixture of polysaccharides whose spatial proximities and interactions with each other are not well understood. We recently obtained the first two-dimensional (2D) and three-dimensional high-resolution magic-angle-spinning 13C solid-state nuclear magnetic resonance spectra of the uniformly 13C-labeled primary cell wall of Arabidopsis thaliana, which allowed us to assign the majority of 13C resonances of the three major classes of polysaccharides: cellulose, hemicellulose, and pectins. In this work, we measured the intensity buildup of 13C-13C cross-peaks in a series of 2D 13C correlation spectra to obtain semiquantitative information about the spatial proximities between different polysaccharides. Comparison of 2D spectra measured at different spin diffusion mixing times identified intermolecular pectin-cellulose cross-peaks as well as interior cellulose-surface cellulose cross-peaks. The intensity buildup time constants are only modestly longer for cellulose-pectin cross-peaks than for interior cellulose-surface cellulose cross-peaks, indicating that pectins come into direct contact with the cellulose microfibrils. Approximately 25-50% of the cellulose chains exhibit close contact with pectins. The 13C magnetization of the wall polysaccharides is not fully equilibrated by 1.5 s, indicating that pectins and cellulose are not homogeneously mixed on the molecular level. We also assigned the 13C signals of cell wall proteins, identifying common residues such as Pro, Hyp, Tyr, and Ala. The chemical shifts indicate significant coil and sheet conformations in these structural proteins. Interestingly, few cross- peaks were observed between the proteins and the polysaccharides. Taken together, these data indicate that the three major types of polysaccharides in the primary wall of Arabidopsis form a single cohesive network, while structural proteins form a relatively separate domain. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
9. Xyloglucan Xylosyltransferases XXT1, XXT2, and XXT5 and the Glucan Synthase CSLC4 Form Golgi-Localized Multiprotein Complexes1[W][OA].
- Author
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Yi Hsiang Chou, Pogorelko, Gennady, and Zabotina, Olga A.
- Subjects
XYLOGLUCANS ,GLUCANS ,GLUCAN synthase ,GOLGI apparatus ,PLANT physiology ,ARABIDOPSIS thaliana ,PHYSIOLOGY - Abstract
Xyloglucan is the major hemicellulosic polysaccharide in the primary cell walls of most vascular dicotyledonous plants and has important structural and physiological functions in plant growth and development. In Arabidopsis (Ambidopsis thaliana), the 1,4-β-glucan synthase, Cellulose Synthase-Like C4 (CSLC4), and three xylosyltransferases, XXT1, XXT2, and XXT5, act in the Golgi to form the xylosylated glucan backbone during xyloglucan biosynthesis. However, the functional organization of these enzymes in the Golgi membrane is currently unknown. In this study, we used bimolecular fluorescence complementation and in vitro pull-down assays to investigate the supramolecular organization of the CSLC4, XXT1, XXT2, and XXT5 proteins in Arabidopsis protoplasts. Quantification of bimolecular fluorescence complementation fluorescence by flow cytometry allowed us to perform competition assays that demonstrated the high probability of protein-protein complex formation in vivo and revealed differences in the abilities of these proteins to form multiprotein complexes. Results of in vitro pull-down assays using recombinant proteins confirmed that the physical interactions among XXTs occur through their catalytic domains. Additionally, coimmunoprecipitation of XXT2YFP and XXT5HA proteins from Arabidopsis protoplasts indicated that while the formation of the XXT2-XXT2 homocomplex involves disulfide bonds, the formation of the XXT2-XXT5 heterocomplex does not involve covalent interactions. The combined data allow us to propose that the proteins involved in xyloglucan biosynthesis function in a multiprotein complex composed of at least two homocomplexes, CSLC4-CSLC4 and XXT2-XXT2, and three heterocomplexes, XXT2-XXT5, XXT1-XXT2, and XXT5-CSLC4. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
10. Coexpression of Fungal Cell Wall-Modifying Enzymes Reveals Their Additive Impact on Arabidopsis Resistance to the Fungal Pathogen, Botrytis cinerea.
- Author
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Swaminathan, Sivakumar, Reem, Nathan T., Lionetti, Vincenzo, and Zabotina, Olga A.
- Subjects
CYTOSKELETON ,PHYTOPATHOGENIC microorganisms ,ASPERGILLUS nidulans ,CELLULAR signal transduction ,ARABIDOPSIS ,PLANT defenses ,PLANT cell walls ,BOTRYTIS cinerea - Abstract
Simple Summary: In the present study, we created transgenic Arabidopsis plants overexpressing two fungal acetylesterases and a fungal feruloylesterase that acts on cell wall polysaccharides and studied their possible complementary additive effects on host defense reactions against the fungal pathogen, Botrytis cinerea. Our results showed that the Arabidopsis plants overexpressing two acetylesterases together contributed significantly higher resistance to B. cinerea in comparison with single protein expression. Conversely, coexpression of either of the acetyl esterases together with feruloylesterase compensates the latter's negative impact on plant resistance. The results also provided evidence that combinatorial coexpression of some cell wall polysaccharide-modifying enzymes might exert an additive effect on plant immune response by constitutively priming plant defense pathways even before pathogen invasion. These findings have potential uses in protecting valuable crops against pathogens. The plant cell wall (CW) is an outer cell skeleton that plays an important role in plant growth and protection against both biotic and abiotic stresses. Signals and molecules produced during host–pathogen interactions have been proven to be involved in plant stress responses initiating signal pathways. Based on our previous research findings, the present study explored the possibility of additively or synergistically increasing plant stress resistance by stacking beneficial genes. In order to prove our hypothesis, we generated transgenic Arabidopsis plants constitutively overexpressing three different Aspergillus nidulans CW-modifying enzymes: a xylan acetylesterase, a rhamnogalacturonan acetylesterase and a feruloylesterase. The two acetylesterases were expressed either together or in combination with the feruloylesterase to study the effect of CW polysaccharide deacetylation and deferuloylation on Arabidopsis defense reactions against a fungal pathogen, Botrytis cinerea. The transgenic Arabidopsis plants expressing two acetylesterases together showed higher CW deacetylation and increased resistance to B. cinerea in comparison to wild-type (WT) Col-0 and plants expressing single acetylesterases. While the expression of feruloylesterase alone compromised plant resistance, coexpression of feruloylesterase together with either one of the two acetylesterases restored plant resistance to the pathogen. These CW modifications induced several defense-related genes in uninfected healthy plants, confirming their impact on plant resistance. These results demonstrated that coexpression of complementary CW-modifying enzymes in different combinations have an additive effect on plant stress response by constitutively priming the plant defense pathways. These findings might be useful for generating valuable crops with higher protections against biotic stresses. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
11. Arabidopsis XXT5 gene encodes a putative α-1,6-xylosyltransferase that is involved in xyloglucan biosynthesis.
- Author
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Zabotina, Olga A., van de Ven, Wilhelmina T. G., Freshour, Glenn, Drakakaki, Georgia, Cavalier, David, Mouille, Gregory, Hahn, Michael G., Keegstra, Kenneth, and Raikhel, Natasha V.
- Subjects
- *
PLANT development , *ARABIDOPSIS thaliana , *GENETIC engineering , *EPITOPES , *POLYSACCHARIDES , *PLANT cells & tissues - Abstract
The function of a putative xyloglucan xylosyltransferase from Arabidopsis thaliana ( At1g74380; XXT5) was studied. The XXT5 gene is expressed in all plant tissues, with higher levels of expression in roots, stems and cauline leaves. A T-DNA insertion in the XXT5 gene generates a readily visible root hair phenotype (root hairs are shorter and form bubble-like extrusions at the tip), and also causes the alteration of the main root cellular morphology. Biochemical characterization of cell wall polysaccharides isolated from xxt5 mutant seedlings demonstrated decreased xyloglucan quantity and reduced glucan backbone substitution with xylosyl residues. Immunohistochemical analyses of xxt5 plants revealed a selective decrease in some xyloglucan epitopes, whereas the distribution patterns of epitopes characteristic for other cell wall polysaccharides remained undisturbed. Transformation of xxt5 plants with a 35S::HA-XXT5 construct resulted in complementation of the morphological, biochemical and immunological phenotypes, restoring xyloglucan content and composition to wild-type levels. These data provide evidence that XXT5 is a xyloglucan α-1,6-xylosyltransferase, and functions in the biosynthesis of xyloglucan. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
12. Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development.
- Author
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Drakakaki, Georgia, Zabotina, Olga, Delgado, Ivan, Robert, Stéphanie, Keegstra, Kenneth, and Raikhel, Natasha
- Subjects
- *
GLYCOPROTEINS , *GROWTH factors , *POLYSACCHARIDES , *BIOSYNTHESIS , *ARABIDOPSIS thaliana , *POLLEN - Abstract
Reversibly glycosylated polypeptides (RGPs) have been implicated in polysaccharide biosynthesis. To date, to our knowledge, no direct evidence exists for the involvement of RGPs in a particular biochemical process. The Arabidopsis (Arabidopsis thaliana) genome contains five RGP genes out of which RGP1 and RGP2 share the highest sequence identity. We characterized the native expression pattern of Arabidopsis RGP1 and RGP2 and used reverse genetics to investigate their respective functions. Although both genes are ubiquitously expressed, the highest levels are observed in actively growing tissues and in mature pollen, in particular. RGPs showed cytoplasmic and transient association with Golgi. In addition, both proteins colocalized in the same compartments and coimmunoprecipitated from plant cell extracts. Single-gene disruptions did not show any obvious morphological defects under greenhouse conditions, whereas the double-insertion mutant could not be recovered. We present evidence that the double mutant is lethal and demonstrate the critical role of RGPs, particularly in pollen development. Detailed analysis demonstrated that mutant pollen development is associated with abnormally enlarged vacuoles and a poorly defined inner cell wall layer, which consequently results in disintegration of the pollen structure during pollen mitosis I. Taken together, our results indicate that RGP1 and RGP2 are required during microspore development and pollen mitosis, either affecting cell division and/or vacuolar integrity. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
13. Post-Synthetic Reduction of Pectin Methylesterification Causes Morphological Abnormalities and Alterations to Stress Response in Arabidopsis thaliana.
- Author
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Reem, Nathan T., Chambers, Lauran, Zhang, Ning, Abdullah, Siti Farah, Chen, Yintong, Feng, Guanhua, Gao, Song, Soto-Burgos, Junmarie, Pogorelko, Gennady, Bassham, Diane C., Anderson, Charles T., Walley, Justin W., and Zabotina, Olga A.
- Subjects
PECTINS ,ARABIDOPSIS thaliana ,CELL communication ,PECTINESTERASE ,PLANT cell walls ,GALACTURONIC acid ,ASPERGILLUS nidulans - Abstract
Pectin is a critical component of the plant cell wall, supporting wall biomechanics and contributing to cell wall signaling in response to stress. The plant cell carefully regulates pectin methylesterification with endogenous pectin methylesterases (PMEs) and their inhibitors (PMEIs) to promote growth and protect against pathogens. We expressed Aspergillus nidulans pectin methylesterase (AnPME) in Arabidopsis thaliana plants to determine the impacts of methylesterification status on pectin function. Plants expressing AnPME had a roughly 50% reduction in methylester content compared with control plants. AnPME plants displayed a severe dwarf phenotype, including small, bushy rosettes and shorter roots. This phenotype was caused by a reduction in cell elongation. Cell wall composition was altered in AnPME plants, with significantly more arabinose and significantly less galacturonic acid, suggesting that plants actively monitor and compensate for altered pectin content. Cell walls of AnPME plants were more readily degraded by polygalacturonase (PG) alone but were less susceptible to treatment with a mixture of PG and PME. AnPME plants were insensitive to osmotic stress, and their susceptibility to Botrytis cinerea was comparable to wild type plants despite their compromised cell walls. This is likely due to upregulated expression of defense response genes observed in AnPME plants. These results demonstrate the importance of pectin in both normal growth and development, and in response to biotic and abiotic stresses. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
14. Xyloglucan Xylosyltransferases XXT1, XXT2, and XXT5 and the Glucan Synthase CSLC4 Form Golgi-Localized Multiprotein Complexes1[W][OA].
- Author
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Yi Hsiang Chou, Pogorelko, Gennady, and Zabotina, Olga A.
- Subjects
- *
XYLOGLUCANS , *GLUCANS , *GLUCAN synthase , *GOLGI apparatus , *PLANT physiology , *ARABIDOPSIS thaliana , *PHYSIOLOGY - Abstract
Xyloglucan is the major hemicellulosic polysaccharide in the primary cell walls of most vascular dicotyledonous plants and has important structural and physiological functions in plant growth and development. In Arabidopsis (Ambidopsis thaliana), the 1,4-β-glucan synthase, Cellulose Synthase-Like C4 (CSLC4), and three xylosyltransferases, XXT1, XXT2, and XXT5, act in the Golgi to form the xylosylated glucan backbone during xyloglucan biosynthesis. However, the functional organization of these enzymes in the Golgi membrane is currently unknown. In this study, we used bimolecular fluorescence complementation and in vitro pull-down assays to investigate the supramolecular organization of the CSLC4, XXT1, XXT2, and XXT5 proteins in Arabidopsis protoplasts. Quantification of bimolecular fluorescence complementation fluorescence by flow cytometry allowed us to perform competition assays that demonstrated the high probability of protein-protein complex formation in vivo and revealed differences in the abilities of these proteins to form multiprotein complexes. Results of in vitro pull-down assays using recombinant proteins confirmed that the physical interactions among XXTs occur through their catalytic domains. Additionally, coimmunoprecipitation of XXT2YFP and XXT5HA proteins from Arabidopsis protoplasts indicated that while the formation of the XXT2-XXT2 homocomplex involves disulfide bonds, the formation of the XXT2-XXT5 heterocomplex does not involve covalent interactions. The combined data allow us to propose that the proteins involved in xyloglucan biosynthesis function in a multiprotein complex composed of at least two homocomplexes, CSLC4-CSLC4 and XXT2-XXT2, and three heterocomplexes, XXT2-XXT5, XXT1-XXT2, and XXT5-CSLC4. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
15. The Arabidopsis domain of unknown function 1218 (DUF1218) containing proteins, MODIFYING WALL LIGNIN-1 and 2 (At1g31720/MWL-1 and At4g19370/MWL-2) function redundantly to alter secondary cell wall lignin content
- Author
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Zabotina, Olga [Iowa State Univ., Ames, IA (United States)]
- Published
- 2016
- Full Text
- View/download PDF
16. Structure and Interactions of Plant Cell-Wall Polysaccharides by Two- and Three-Dimensional Magic-Angle-Spinning Solid-State NMR.
- Author
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Dick-Pérez, Marilú, Yuan Zhang, Hayes, Jennifer, Salazar, Andre, Zabotina, Olga A., and Mei Hong
- Subjects
- *
PLANT cell walls , *POLYSACCHARIDES , *PLANT growth , *ARABIDOPSIS thaliana , *NUCLEAR magnetic resonance , *MICROFIBRILS , *PECTINS - Abstract
The polysaccharide-rich cell walls (CWs) of plants perform essential functions such as maintaining tensile strength and allowing plant growth. Using two- and three-dimensional magic-angle-spinning (MAS) solid-state NMR and uniformly 13C-labeled Arabidopsis thaliana, we have assigned the resonances of the major polysaccharides in the intact and insoluble primary CW and determined the intermolecular contacts and dynamics of cellulose, hemicelluloses, and pectins. Cellulose microfibrils showed extensive interactions with pectins, while the main hemicellulose, xyloglucan, exhibited few cellulose cross-peaks, suggesting limited entrapment in the microfibrils rather than extensive surface coating. Site-resolved 13C T1 and ±H T1ρ relaxation times indicate that the entrapped xyloglucan has motional properties that are intermediate between the rigid cellulose and the dynamic pectins. Xyloglucan absence in a triple knockout mutant caused the polysaccharides to undergo much faster motions than in the wild-type CW. These results suggest that load bearing in plant CWs is accomplished by a single network of all three types of polysaccharides instead of a cellulose-xyloglucan network, thus revising the existing paradigm of CW structure. The extensive pectin-cellulose interaction suggests a central role for pectins in maintaining the structure and function of plant CWs. This study demonstrates the power of multidimensional MAS NMR for molecular level investigation of the structure and dynamics of complex and energy-rich plant materials. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
17. The Arabidopsis Domain of Unknown Function 1218 (DUF1218) Containing Proteins, MODIFYING WALL LIGNIN-1 and 2 (At1g31720/MWL-1 and At4g19370/MWL-2) Function Redundantly to Alter Secondary Cell Wall Lignin Content
- Author
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Berdine Coetzee, Shawn D. Mansfield, Ritesh Mewalal, Alexander Andrew Myburg, Eshchar Mizrachi, and Zabotina, Olga A
- Subjects
0106 biological sciences ,0301 basic medicine ,Cell Membranes ,Mutant ,Arabidopsis ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Plant Science ,Polymerase Chain Reaction ,Lignin ,01 natural sciences ,Cell membrane ,Gene Knockout Techniques ,Gene Expression Regulation, Plant ,Cell Wall ,Electrochemistry ,Arabidopsis thaliana ,Developmental ,lcsh:Science ,Phylogeny ,Microscopy ,Microscopy, Confocal ,Multidisciplinary ,Plant Stems ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Regulation, Developmental ,Plants ,Chemistry ,medicine.anatomical_structure ,Biochemistry ,Confocal ,Physical Sciences ,Hyperexpression Techniques ,Cellular Structures and Organelles ,Plant Cell Walls ,Cellular Types ,Secondary cell wall ,Research Article ,Secondary Cells ,Protein family ,General Science & Technology ,Plant Cell Biology ,Arabidopsis Thaliana ,Brassica ,Research and Analysis Methods ,Cell wall ,03 medical and health sciences ,Cell Walls ,Model Organisms ,Plant and Algal Models ,Plant Cells ,Gene Expression and Vector Techniques ,Genetics ,medicine ,Molecular Biology Techniques ,Molecular Biology ,Molecular Biology Assays and Analysis Techniques ,Arabidopsis Proteins ,lcsh:R ,Cell Membrane ,Chemical Compounds ,Organisms ,Biology and Life Sciences ,Membrane Proteins ,Cell Biology ,Reverse Transcriptase-Polymerase Chain Reaction ,Plant ,biology.organism_classification ,Electrochemical Cells ,030104 developmental biology ,Gene Expression Regulation ,Membrane protein ,Mutation ,lcsh:Q ,010606 plant biology & botany - Abstract
DUF1218 is a land plant-specific innovation and has previously been shown to be associated with cell wall biology, vasculature patterning and abiotic/biotic stress response. The Arabidopsis genome encodes 15 members, two of which (At1g31720 and At4g27435) are preferentially expressed in the secondary cell wall depositing inflorescence stems. To further our understanding of the roles of DUF1218-containing proteins in secondary cell wall biology, we functionally characterized At1g31720 (herein referred to as MODIFYING WALL LIGNIN-1 or MWL-1). Since related gene family members may contribute to functional redundancy, we also characterized At4g19370 (MWL-2), the most closely related gene to MWL-1 in the protein family. Subcellular localization revealed that both Arabidopsis proteins are targeted to the cell periphery. The single T-DNA knockout lines, mwl-1 and mwl-2, and independent overexpression lines showed no significant differences in plant growth or changes in total lignin content relative to wild-type (WT) control plants. However, the double homozygous mutant, mwl-1/mwl-2, had smaller rosettes with a significant decrease in rosette fresh weight and stem height relative to the WT control at four weeks and six weeks, respectively. Moreover, mwl-1/mwl-2 showed a significant reduction in total lignin content (by ca. 11% relative to WT) and an increase in syringyl/guaiacyl (S/G) monomer ratio relative to the control plants. Our study has identified two additional members of the DUF1218 family in Arabidopsis as novel contributors to secondary cell wall biology, specifically lignin biosynthesis, and these proteins appear to function redundantly.
- Published
- 2016
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