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Assessing past foodways, subsistence strategies, and environments depends on the accurate identification of animals in the archaeological record. The high rates of fragmentation and often poor preservation of animal bones at many archaeological sites across sub-Saharan Africa have rendered archaeofaunal specimens unidentifiable beyond broad categories, such as "large mammal" or "medium bovid". Identification of archaeofaunal specimens through Zooarchaeology by Mass Spectrometry (ZooMS), or peptide mass fingerprinting of bone collagen, offers an avenue for identification of morphologically ambiguous or unidentifiable bone fragments from such assemblages. However, application of ZooMS analysis has been hindered by a lack of complete reference peptide markers for African taxa, particularly bovids. Here we present the complete set of confirmed ZooMS peptide markers for members of all African bovid tribes. We also identify two novel peptide markers that can be used to further distinguish between bovid groups. We demonstrate that nearly all African bovid subfamilies are distinguishable using ZooMS methods, and some differences exist between tribes or sub-tribes, as is the case for Bovina (cattle) vs. Bubalina (African buffalo) within the subfamily Bovinae. We use ZooMS analysis to identify specimens from extremely fragmented faunal assemblages from six Late Holocene archaeological sites in Zambia. ZooMS-based identifications reveal greater taxonomic richness than analyses based solely on morphology, and these new identifications illuminate Iron Age subsistence economies c. 2200-500 cal BP. While the Iron Age in Zambia is associated with the transition from hunting and foraging to the development of farming and herding, our results demonstrate the continued reliance on wild bovids among Iron Age communities in central and southwestern Zambia Iron Age and herding focused primarily on cattle. We also outline further potential applications of ZooMS in African archaeology., Competing Interests: The authors have declared that no competing interests exist. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Human-mediated biological exchange has had global social and ecological impacts. In sub-Saharan Africa, several domestic and commensal animals were introduced from Asia in the pre-modern period; however, the timing and nature of these introductions remain contentious. One model supports introduction to the eastern African coast after the mid-first millennium CE, while another posits introduction dating back to 3000 BCE. These distinct scenarios have implications for understanding the emergence of long-distance maritime connectivity, and the ecological and economic impacts of introduced species. Resolution of this longstanding debate requires new efforts, given the lack of well-dated fauna from high-precision excavations, and ambiguous osteomorphological identifications. We analysed faunal remains from 22 eastern African sites spanning a wide geographic and chronological range, and applied biomolecular techniques to confirm identifications of two Asian taxa: domestic chicken (Gallus gallus) and black rat (Rattus rattus). Our approach included ancient DNA (aDNA) analysis aided by BLAST-based bioinformatics, Zooarchaeology by Mass Spectrometry (ZooMS) collagen fingerprinting, and direct AMS (accelerator mass spectrometry) radiocarbon dating. Our results support a late, mid-first millennium CE introduction of these species. We discuss the implications of our findings for models of biological exchange, and emphasize the applicability of our approach to tropical areas with poor bone preservation. [ABSTRACT FROM AUTHOR]

Archaeological and environmental research by an international and interdisciplinary team opens new perspectives into the settlement histories of Kansyore, Early Iron Age, and Bigo period peoples in the once forested regions of the Ndali Crater Lakes Region (NCLR) of western Uganda. The research examines the role of Kansyore agropastoralists and their Early Iron Age and Bantu-speaking contemporaries in remaking a once forested environment into a forest-savannah mosaic from circa 500 BC to the end of the first millennium AD. Archaeological settlement and subsistence evidence is examined within a framework of social interaction of Sudanic speakers with Bantu speakers, drawing on historical linguistics and environmental studies to arrive at a new synthesis of late Holocene history in western Uganda. This perspective also unveils the significance and chronology of Boudiné ware, a long enigmatic ceramic tradition that we identify as contemporary to Transitional Urewe and deeply influenced through social interactions with those making Kansyore ceramics and inhabiting the same landscape. Using archaeological evidence from fifteen sites and multiple burials spanning from 400 to 1650 calAD, new views of ceramic histories, lifeways, and symbolic values are revealed, including Bigo period settlements that arose in what was an environmental refugium beginning in the early fourteenth century AD. This research also shows that the Kansyore of the forested region east of the Rwenzori Mountains had greater affinities to late Holocene archaeological evidence from western Equatoria, in the southern South Sudan, and Kansyore Island, Uganda, than it does to the Kansyore in eastern Kenya. [ABSTRACT FROM AUTHOR]